MedPath

Study Investigating a PEGylated Recombinant Factor VIII (BAX 855) for Hemophilia A (PROLONG-ATE Study)

Phase 2
Completed
Conditions
Hemophilia A
Interventions
Biological: Antihemophilic Factor (Recombinant) - Plasma/Albumin Free Method
Biological: PEGylated Recombinant Factor VIII
Registration Number
NCT01736475
Lead Sponsor
Baxalta now part of Shire
Brief Summary

To assess efficacy and safety, including immunogenicity of BAX 855 administered as prophylaxis and as on-demand therapy in adult and adolescent (12-65 years) previously treated patients (PTPs) with severe hemophilia A To determine the pharmacokinetic (PK) parameters of BAX 855.

Detailed Description

Not available

Recruitment & Eligibility

Status
COMPLETED
Sex
Male
Target Recruitment
159
Inclusion Criteria
  • Participant and/or legal representative has/have voluntarily provided signed informed consent
  • Participant is 12 to 65 years old at the time of screening
  • Participant is male with severe hemophilia A (Factor VIII (FVIII) clotting activity < 1%) as confirmed by central laboratory at screening after the appropriate washout period or a documented FVIII clotting activity <1%
  • Participant has been previously treated with plasma-derived FVIII concentrates or recombinant FVIII for ≥150 documented exposure days (EDs)
  • Participant is currently receiving prophylaxis or on-demand therapy with FVIII
  • Participant is willing and able to comply with the requirements of the protocol

Main

Exclusion Criteria
  • Participant has detectable FVIII inhibitory antibodies (≥ 0.6 Bethesda Units (BU) using the Nijmegen modification of the Bethesda assay) as confirmed by central laboratory at screening
  • Participant has history of FVIII inhibitory antibodies (≥ 0.4 BU using the Nijmegen modification of the Bethesda assay or ≥ 0.6 BU using the Bethesda assay) at any time prior to screening
  • Participant has been diagnosed with an inherited or acquired hemostatic defect other than hemophilia A (eg, qualitative platelet defect or von Willebrand's disease).

Study & Design

Study Type
INTERVENTIONAL
Study Design
PARALLEL
Arm && Interventions
GroupInterventionDescription
ProphylaxisPEGylated Recombinant Factor VIII-
On-demandPEGylated Recombinant Factor VIII-
ProphylaxisAntihemophilic Factor (Recombinant) - Plasma/Albumin Free Method-
Primary Outcome Measures
NameTimeMethod
Annualized Bleeding Rate (ABR)9 months

Comparisons between prophylactic and on-demand treatment were based on ABR estimates from a negative binomial regression model, taking into account the treatment regimen, target joints and age at screening, and duration of the observation period for efficacy.

Secondary Outcome Measures
NameTimeMethod
Weight-adjusted Consumption of BAX 855 - Per Prophylactic Infusion and Pharmacokinetic (PK) InfusionProphylactic Infusion: ≥50 exposure days or 6 months (±2 weeks), whichever occurs last. PK Infusion: PK #1 Pre-infusion within 30 minutes; Post-infusion 10 min, and 0.5, 1, 3, 6, 24, 32, 48, 56 hours (h). PK #2 also at Post-infusion 96h
Patient Reported Outcomes - Short Form (SF)-36, Change From Baseline to End of StudyBaseline; and end of study visit [at least 50 exposure days or 6 months (±2 weeks), whichever occurs last, for the prophylaxis arm and 6 months (± 2 weeks) for the on-demand arm]

Change from Baseline to End of Study for SF-36 Questionnaire is provided. Scores for individual SF-36 categories range from 0 to 100 with higher scores representing better health. Given that higher scores indicate better health-related quality of life (HRQoL) and that the change scores were calculated as the value at study completion minus the value at baseline, a negative change score indicates a worsening of HRQoL.

Average Number of BAX 855 Infusions Needed for the Treatment of Bleeding EpisodesFrom first exposure to BAX 855 until the end of the study, [at least 50 exposure days or 6 months (±2 weeks), whichever occurs last, for the prophylaxis arm; and 6 months (± 2 weeks) for the on-demand arm].
Weight-adjusted Consumption of BAX 855 - Per Treatment of Bleeding Episode (BE) and Per BE for Maintenance of HemostasisTreatment of Bleeding Episode (BE): Minor/Moderate BE every 12 to 24 hours until bleeding is resolved; Major BE every 8 to 12 hours until bleeding is resolved. Per BE for Maintenance of Hemostasis: within 48 hours after bleeding episode resolution.

Infusions per bleeding episode for maintenance of hemostasis only includes infusions following the resolution of a bleed to maintain hemostasis.

Rate of Success of BAX 855 for Treatment of Bleeding EpisodesAt least 50 exposure days or 6 months (±2 weeks), whichever occurs last, for the prophylaxis arm and 6 months (± 2 weeks) for the on-demand arm.

Success in the control of bleeding was defined as a rating of excellent or good using the Efficacy Rating Scale for Treatment of Bleeding Episodes measured 24 hours after initiation of treatment for the bleeding episode. EXCELLENT: Full relief of pain and cessation of objective signs of bleeding (eg, swelling, tenderness, and decreased range of motion in the case of musculoskeletal hemorrhage) after a single infusion. No additional infusion is required for the control of bleeding. Administration of further infusions to maintain hemostasis would not affect this scoring. GOOD: Definite pain relief and/or improvement in signs of bleeding after a single infusion. Possibly requires more than 1 infusion for complete resolution. FAIR: Probable and/or slight relief of pain and slight improvement in signs of bleeding after a single infusion. Required more than 1 infusion for complete resolution. NONE: No improvement or condition worsens.

Percentage of Participants With Adverse EventsFrom first exposure to BAX 855 until the end of the study, [at least 50 exposure days or 6 months (±2 weeks), whichever occurs last, for the prophylaxis arm; and 6 months (± 2 weeks) for the on-demand arm].

Adverse Events (AEs) and Serious Adverse Events (SAEs)

Pharmacokinetics (Pk) - Apparent Volume of Distribution at Steady State (Vss) (One-stage Clotting Assay)Within 30 minutes prior to start of infusion; and post-infusion at 10, 30 minutes, and 1, 3, 6, 9, 24, 32, 48, 56, 72 (PK2 and PK3 only), and 96 hours (PK2 and PK3 only).

The apparent volume of distribution at steady state (Vss) will be calculated as: Vss = Clearance \* Mean Residence Time. Participants in the pharmacokinetic full analysis set (PKFAS) analysis set received an initial infusion of ADVATE for pharmacokinetic analysis (PK-1) followed by a washout period and an infusion of BAX 855 for a second pharmacokinetic analysis (PK-2). After at least 50 EDs of BAX 855, participants in the PK subgroup received another infusion of BAX 855 for pharmacokinetic analysis (PK-3).

Change in Vital Signs From Screening - Respiratory RateScreening, week 2, week 4, exposure day 10-15, month 3, study completion/termination
Changes in Vital Signs From Screening - Blood PressureScreening, week 2, week 4, exposure day 10-15, month 3, study completion/termination

Systolic Blood Pressure (SBP) Diastolic Blood Pressure (DBP)

Changes in Clinical Chemistry Laboratory Assessments From Screening - Bicarbonate, Chloride, Glucose, Potassium, Sodium, Blood Urea Nitrogen (BUN)Screening, week 2, week 4, month 3, study completion/termination
Number of Participants With ≤1, 2, 3, 4, 5, 6, or >6 Month Time Intervals Between Bleeding Episodes or no Bleeding EpisodesFrom first exposure to BAX 855 until the end of the study, [at least 50 exposure days or 6 months (±2 weeks), whichever occurs last, for the prophylaxis arm; and 6 months (± 2 weeks) for the on-demand arm].

Interval between Bleeds in months was calculated as: Observation period for efficacy (in days)/(number of bleeds)\*(12/365.2425)

Immunogenicity - Number of Participants With Positive Inhibitory Antibodies to FVIII, Binding Antibodies to FVIII, PEG-VIII, PEG and Anti-CHO Antibodies at Study Completion/TerminationFrom first exposure to BAX 855 until the end of the study, [at least 50 exposure days or 6 months (±2 weeks), whichever occurs last, for the prophylaxis arm; and 6 months (± 2 weeks) for the on-demand arm].

Number of participants who received BAX855, with immunogenicity data from study completion/termination visit. FVIII = factor VIII; PEG-VIII = polyethylene glycol-factor VIII; Anti-CHO = Anti-Chinese hamster ovary

Patient Reported Outcomes: Haemo-SYM Questionnaire, Change in Score From Baseline to End of StudyBaseline; and end of study visit [at least 50 exposure days or 6 months (±2 weeks), whichever occurs last, for the prophylaxis arm and 6 months (± 2 weeks) for the on-demand arm].

The HAEMO-SYM has two subscales: pain and bleeds. HAEMO-SYM subscale scores are calculated by taking the mean of the items in each subscale and transforming them to a 0 (none or absent) to 100 (very severe) scale. Given that higher scores indicate more severe symptoms on the Haemo-SYM and that the change scores were calculated as the value at study completion minus the value at baseline, a negative change score indicates an improvement (reduction in symptoms). Conversely, a positive change score indicates worsening symptoms.

Pharmacokinetics (Pk) - Area Under the Concentration Versus Time Curve From 0 to Infinity (AUC0-∞) (One-stage Clotting Assay)Within 30 minutes prior to start of infusion; and post-infusion at 10, 30 minutes, and 1, 3, 6, 9, 24, 32, 48, 56, 72 (PK2 and PK3 only), and 96 hours (PK2 and PK3 only).

Calculated by WinNonlin NCA (Model 201, calculation method: Linear Trapezoidal Linear/Log Interpolation). Participants in the pharmacokinetic full analysis set (PKFAS) analysis set received an initial infusion of ADVATE for pharmacokinetic analysis (PK-1) followed by a washout period and an infusion of BAX 855 for a second pharmacokinetic analysis (PK-2). After at least 50 EDs of BAX 855, participants in the PK subgroup received another infusion of BAX 855 for pharmacokinetic analysis (PK-3).

Pharmacokinetics (Pk) -Time to Maximum Concentration in Plasma (Tmax) (One-stage Clotting Assay)Within 30 minutes prior to start of infusion; and post-infusion at 10, 30 minutes, and 1, 3, 6, 9, 24, 32, 48, 56, 72 (PK2 and PK3 only), and 96 hours (PK2 and PK3 only).

Tmax in hours will be defined as the time to reach Cmax. Participants in the pharmacokinetic full analysis set (PKFAS) analysis set received an initial infusion of ADVATE for pharmacokinetic analysis (PK-1) followed by a washout period and an infusion of BAX 855 for a second pharmacokinetic analysis (PK-2). After at least 50 EDs of BAX 855, participants in the PK subgroup received another infusion of BAX 855 for pharmacokinetic analysis (PK-3).

Change in Vital Signs From Screening - TemperatureScreening, week 2, week 4, exposure day 10-15, month 3, study completion/termination
Changes in Hematology Laboratory Assessments From Screening - HemoglobinScreening, week 2, week 4, month 3, study completion/termination
Pharmacokinetics (Pk) - Plasma Half-life (One-stage Clotting Assay)Within 30 minutes prior to start of infusion; and post-infusion at 10, 30 minutes, and 1, 3, 6, 9, 24, 32, 48, 56, 72 (PK2 and PK3 only), and 96 hours (PK2 and PK3 only).

Terminal half-life calculated as log_e2/λz where λz is the terminal elimination rate constant. Participants in the pharmacokinetic full analysis set (PKFAS) analysis set received an initial infusion of ADVATE for pharmacokinetic analysis (PK-1) followed by a washout period and an infusion of BAX 855 for a second pharmacokinetic analysis (PK-2). After at least 50 EDs of BAX 855, participants in the PK subgroup received another infusion of BAX 855 for pharmacokinetic analysis (PK-3).

Pharmacokinetics (Pk) - Total Body Clearance (One-stage Clotting Assay)Within 30 minutes prior to start of infusion; and post-infusion at 10, 30 minutes, and 1, 3, 6, 9, 24, 32, 48, 56, 72 (PK2 and PK3 only), and 96 hours (PK2 and PK3 only).

Clearance in dL/(kg.h) will be calculated as the dose in IU/kg divided by the total area under the curve starting from the begin of infusion (or the end of infusion if start time is not available). Participants in the pharmacokinetic full analysis set (PKFAS) analysis set received an initial infusion of ADVATE for pharmacokinetic analysis (PK-1) followed by a washout period and an infusion of BAX 855 for a second pharmacokinetic analysis (PK-2). After at least 50 EDs of BAX 855, participants in the PK subgroup received another infusion of BAX 855 for pharmacokinetic analysis (PK-3).

Pharmacokinetics (Pk) - Maximum Plasma Concentration (Cmax) (One-stage Clotting Assay)Within 30 minutes prior to start of infusion; and post-infusion at 10, 30 minutes, and 1, 3, 6, 9, 24, 32, 48, 56, 72 (PK2 and PK3 only), and 96 hours (PK2 and PK3 only).

Participants in the pharmacokinetic full analysis set (PKFAS) analysis set received an initial infusion of ADVATE for pharmacokinetic analysis (PK-1) followed by a washout period and an infusion of BAX 855 for a second pharmacokinetic analysis (PK-2). After at least 50 EDs of BAX 855, participants in the PK subgroup received another infusion of BAX 855 for pharmacokinetic analysis (PK-3).

Changes in Clinical Chemistry Laboratory Assessments From Screening - Creatinine, and BilirubinScreening, week 2, week 4, month 3, study completion/termination
Pharmacokinetics (Pk) - Mean Residence Time (One-stage Clotting Assay)Within 30 minutes prior to start of infusion; and post-infusion at 10, 30 minutes, and 1, 3, 6, 9, 24, 32, 48, 56, 72 (PK2 and PK3 only), and 96 hours (PK2 and PK3 only).

The mean residence time (MRT) w as calculated as total area under the moment curve divided by the total area under the curve starting from the begin of infusion (or the end of infusion if start time is not available). Participants in the pharmacokinetic full analysis set (PKFAS) analysis set received an initial infusion of ADVATE for pharmacokinetic analysis (PK-1) followed by a washout period and an infusion of BAX 855 for a second pharmacokinetic analysis (PK-2). After at least 50 EDs of BAX 855, participants in the PK subgroup received another infusion of BAX 855 for pharmacokinetic analysis (PK-3).

Changes in Clinical Chemistry Laboratory Assessments From Screening - Albumin and ProteinScreening, week 2, week 4, month 3, study completion/termination
Pharmacokinetics (Pk) - Incremental Recovery Over Time (One-stage Clotting Assay)Within 30 minutes prior to start of infusion; and post-infusion at 10, 30 minutes, and 1, 3, 6, 9, 24, 32, 48, 56, 72 (PK2 and PK3 only), and 96 hours (PK2 and PK3 only).

Incremental recovery (IR) in (IU/dL)/ (IU/kg) calculated as: IR = (Cmax- (C pre-infusion)) / (Dose/kg), where C =concentration. Participants in the pharmacokinetic full analysis set (PKFAS) analysis set received an initial infusion of ADVATE for pharmacokinetic analysis (PK-1) followed by a washout period and an infusion of BAX 855 for a second pharmacokinetic analysis (PK-2). After at least 50 EDs of BAX 855, participants in the PK subgroup received another infusion of BAX 855 for pharmacokinetic analysis (PK-3).

Change in Vital Signs From Screening - Pulse RateScreening, week 2, week 4, exposure day 10-15, month 3, study completion/termination
Changes in Clinical Chemistry Laboratory Assessments From Screening - Alkaline Phosphatase, Alanine Aminotransferase, Aspartate AminotransferaseScreening, week 2, week 4, month 3, study completion/termination

Alkaline Phosphatase (Alk Phos); Alanine Aminotransferase (Ala Amino); Aspartate Aminotransferase (Asp Amino)

Changes in Lipid Panel Assessments From Screening - Cholesterol; High Density Lipoprotein (HDL); Low Density Lipoprotein (LDL); Triglycerides; and Very Low Density Lipoprotein (VLDL)Screening, week 2, week 4, month 3, study completion/termination
Changes in Hematology Laboratory Assessments From Screening - Basophils, Eosinophils, Lymphocytes, Monocytes, Neutrophils, Platelets, and LeukocytesScreening, week 2, week 4, month 3, study completion/termination
Changes in Hematology Laboratory Assessments From Screening - HematocritScreening, week 2, week 4, month 3, study completion/termination
Changes in Hematology Laboratory Assessments From Screening - ErythrocytesScreening, week 2, week 4, month 3, study completion/termination

Trial Locations

Locations (72)

Wojewodzki Szpital Specjalistyczny im.M.Kopernika w Lodzi

🇵🇱

Lodz, Poland

Tri-Service General Hospital

🇨🇳

Taipei, Taiwan

Palmetto Health

🇺🇸

Columbia, South Carolina, United States

AKH - Medizinische Universität Wien

🇦🇹

Vienna, Austria

SHAT of Oncohaematology Diseases

🇧🇬

Sofia, Bulgaria

SI V.K.Gusak Emergency and Reconstructive Surgery Institute of NAMSU Center of IT

🇺🇦

Donetsk, Ukraine

Fremantle Hospital

🇦🇺

Fremantle, Western Australia, Australia

Hollywood Specialist Centre

🇦🇺

Nedlands, Western Australia, Australia

Children's Healthcare of Atlanta

🇺🇸

Atlanta, Georgia, United States

Duke University Medical Center

🇺🇸

Durham, North Carolina, United States

Cincinnati Children's Hospital Medical Center

🇺🇸

Cincinnati, Ohio, United States

The Cleveland Clinic Foundation

🇺🇸

Cleveland, Ohio, United States

University of Utah Health Sciences Center

🇺🇸

Salt Lake City, Utah, United States

Puget Sound Blood Group

🇺🇸

Seattle, Washington, United States

University of Colorado

🇺🇸

Aurora, Colorado, United States

Bleeding and Clotting Disorders Institute

🇺🇸

Peoria, Illinois, United States

Weill Cornell Medical College-New York Presbyterian Hospital

🇺🇸

New York, New York, United States

The Alfred Hospital

🇦🇺

Clayton, Victoria, Australia

Royal Adelaide Hospital

🇦🇺

Adelaide, South Australia, Australia

Landes-Frauen-und Kinderklinik Linz

🇦🇹

Linz, Austria

Fakultni nemocnice Brno

🇨🇿

Brno, Czechia

Fakultni nemocnice Olomouc

🇨🇿

Olomouc, Czechia

Fakultni nemocnice v Motole

🇨🇿

Praha 5, Czechia

Gerinnungszentrum Rhein-Ruhr

🇩🇪

Duisburg, Nordrhein Westfalen, Germany

Rambam Health Care Campus

🇮🇱

Haifa, Israel

Universitaetsklinikum Hamburg-Eppendorf

🇩🇪

Hamburg, Germany

Vivantes Klinikum im Friedrichshain - Landsberger Allee

🇩🇪

Berlin, Germany

Werlhof-Institut MVZ

🇩🇪

Hannover, Germany

Nagoya University Hospital

🇯🇵

Nagoya-shi, Aichi-Ken, Japan

Chaim Sheba Medical Center

🇮🇱

Tel Aviv, Israel

University of Occupational and Environmental Health Hospital

🇯🇵

Kitakyushu-shi, Fukuoka-Ken, Japan

Hyogo College of Medicine Hospital

🇯🇵

Nishinomiya-shi, Hyogo-Ken, Japan

St. Marianna University School of Medicine Hospital

🇯🇵

Kawasaki-shi, Kanagawa-Ken, Japan

Skånes Universitetssjukhus, Malmö

🇸🇪

Malmö, Sweden

Hiroshima University Hospital

🇯🇵

Hiroshima-shi, Hiroshima-Ken, Japan

Nara Medical University Hospital

🇯🇵

Kashihara-shi, Nara-Ken, Japan

Ogikubo Hospital

🇯🇵

Suginami-ku, Tokyo-To, Japan

Tokyo Medical University Hospital

🇯🇵

Shinjuku-ku, Tokyo-To, Japan

Eulji University Hospital

🇰🇷

Daejeon, Korea, Republic of

Pusan National University Hospital

🇰🇷

Busan, Korea, Republic of

Ulsan University Hospital

🇰🇷

Ulsan, Korea, Republic of

Chonnam National University Hwasun Hospital

🇰🇷

Hwasun, Jeollanam-do, Korea, Republic of

Vilnius University Hospital Santariskiu Clinics, Public Institution

🇱🇹

Vilnius, Lithuania

Children's Hospital, Affiliate of Vilnius University Hospital Santariskiu Klinikos

🇱🇹

Vilnius, Lithuania

Kyung hee University Hospital at Gangdong

🇰🇷

Seoul, Korea, Republic of

Hospital Pulau Pinang

🇲🇾

George Town, Pulau Pinang, Malaysia

Hospital Tengku Ampuan Rahimah

🇲🇾

Klang, Selangor, Malaysia

Pusat Darah Negara

🇲🇾

Kuala Lumpur, Malaysia

Academisch Medisch Centrum

🇳🇱

Amsterdam, Netherlands

Uniwersyteckie Centrum Kliniczne

🇵🇱

Gdansk, Poland

Sanador SRL

🇷🇴

Bucuresti, Romania

Complejo Hospitalario Universitario A Coruña

🇪🇸

A Coruña, La Coruña, Spain

Hospital Regional Universitario de Malaga

🇪🇸

Malaga, Málaga, Spain

Karolinska Universitetssjukhuset, Solna

🇸🇪

Stockholm, Sweden

Hospital Universitari Son Espases

🇪🇸

Palma de Mallorca, Baleares, Spain

Hospital Universitari i Politecnic La Fe

🇪🇸

Valencia, Spain

Universitatsspital Zurich

🇨🇭

Zurich, Switzerland

SI Institute of Blood Pathology and Transfusion Medicine of NAMSU

🇺🇦

Lviv, Ukraine

Bristol Royal Hospital for Children

🇬🇧

Bristol, Avon, United Kingdom

Manchester Royal Infirmary

🇬🇧

Manchester, Greater Manchester, United Kingdom

Royal Free Hospital

🇬🇧

London, Greater London, United Kingdom

Royal Manchester Children's Hospital

🇬🇧

Manchester, Greater Manchester, United Kingdom

Churchill Hospital

🇬🇧

Oxford, Oxfordshire, United Kingdom

Leicester Royal Infirmary

🇬🇧

Leicester, Leicestershire, United Kingdom

Penn State Milton S. Hershey Medical Center

🇺🇸

Hershey, Pennsylvania, United States

University of Florida, College of Medicine

🇺🇸

Gainesville, Florida, United States

Tulane University Medical School

🇺🇸

New Orleans, Louisiana, United States

University of North Carolina Chapel Hill

🇺🇸

Chapel Hill, North Carolina, United States

University of Kentucky Medical Center

🇺🇸

Lexington, Kentucky, United States

Children's Mercy Hospitals & Clinics

🇺🇸

Kansas City, Missouri, United States

University of Louisville Hospital

🇺🇸

Louisville, Kentucky, United States

St Thomas' Hospital

🇬🇧

London, Greater London, United Kingdom

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