The Effects of Mannose Supplementation on Glycosylation and Protein Levels in Human Sera
- Conditions
- Physiological Stress
- Interventions
- Dietary Supplement: Mannose
- Registration Number
- NCT06507735
- Lead Sponsor
- University of Texas Southwestern Medical Center
- Brief Summary
The purpose of our study is to investigate the effects of supplemental mannose on sera of humans who are healthy. We will perform proteomics studies to elucidate changes to glycan structure and protein level changes. We hypothesize that we may find that mannose supplies more properly glycosylated protein substrate and helps expedite protein synthesis, that excess mannose leads to alternative glycan structures which confer improved stability, or that there are new sites that become glycosylated after mannose supplementation.
- Detailed Description
Subjects will be enrolled and a baseline blood sample taken. Subjects will take mannose supplements for 1 week (3-4 packets of a standard dose in water), then a follow up blood sample will be taken.
Subject blood will be analyzed by proteomic analysis for 1) change in glycan structure or 2) other protein based approaches to measure changes in levels of proteins.
Recruitment & Eligibility
- Status
- COMPLETED
- Sex
- All
- Target Recruitment
- 6
- At least 18 years of age
- No known medical conditions
- Taking no medications or nutritional supplements
- Agree to the study and provide informed consent
- Less than 18 years of age
- Known medical diagnosis, including pregnancy
- Presently taking medications and/or nutritional supplements
- Do not provide consent
Study & Design
- Study Type
- INTERVENTIONAL
- Study Design
- SINGLE_GROUP
- Arm && Interventions
Group Intervention Description Mannose supplementation Mannose Mannose supplementation will be given and subjects will be studied at baseline and after treatment.
- Primary Outcome Measures
Name Time Method Changes to glycan structure ~1 month Glycopeptide mass spectrometry will be applied to the major glycoproteins present in serum.
Change in glycoprotein levels ~1 month Standard laboratory methods such as ELISA in either research or clinical laboratory settings (Roche Cobas or similar platform).
- Secondary Outcome Measures
Name Time Method
Trial Locations
- Locations (1)
University of Texas Southwestern Medical Center
🇺🇸Dallas, Texas, United States