Effects of Exercise Intensity and Rest Intervals on Intracellular Signals and Anabolic Response of Skeletal Muscle to Resistance Training
试验速览
- 阶段
- 不适用
- 状态
- 已完成
- 发起方
- 入组人数
- 9
- 试验地点
- 2
- 主要终点
- IGF-1 mRNA (messenger ribonucleic acid) in skeletal muscle tissue measured by Quantitative polymerase chain reaction (qPCR)
研究概览
简要总结
Resistance training (RT) is one of the most important stimuli for muscle hypertrophy and it plays an important role on weight loss and fatty acid oxidation increase. RT affects anabolic pathways but the differences among various training techniques has been till now were poorly investigated. The aim of this study was to compare the effect of two different intensity of training, high-intensity interval resistance training (HIIRT) and traditional resistance training (TRT), on muscle signalling pathway.
详细描述
Nine young healthy subjects performed HIIRT and TRT protocol in two different moments and with different legs. HIIRT technique consisted of three sets of: 6 repetitions at 6RM (6 repetitions maximum) and then 20 seconds of rest and 2/3 repetitions (until exhaustion) repeated for 3 times with 2'30" rest between sets; while TRT consisted of 3 sets of 15 reps with 75 sec of rest between sets. Biopsies from the vastus lateralis were taken at baseline (pre), immediately (0h) at the end of training, 6 hours (6h) and 24 hours (24h) after training. Western blot and RT-PCR mRNA (Reverse transcriptase-polymerase chain reaction) analysis were performed to assess muscle signalling pathway activation
研究设计
- 研究类型
- Interventional
- 分配方式
- Randomized
- 干预模型
- Crossover
- 主要目的
- Basic Science
- 盲法
- Single (Outcomes Assessor)
盲法说明
The samples analysed were masked by a number without any indication of the arm.
入排标准
- 年龄范围
- 20 Years 至 30 Years（Adult）
- 性别
- All
- 接受健康志愿者
- 是
入选标准
- •moderate active
- •experience with resistance training
排除标准
- •history of recent myocardial infarction,
- •severe cardiac arrhythmia,
- •unstable angina,
- •poorly controlled hypertension,
- •poorly controlled diabetes mellitus,
- •frequent or complex ventricular ectopy which might interfere with one's ability to adhere to exercise protocols
结局指标
主要结局
IGF-1 mRNA (messenger ribonucleic acid) in skeletal muscle tissue measured by Quantitative polymerase chain reaction (qPCR)
时间窗: 24 hours
IGF-1 mRNA in skeletal muscle tissue taken by biopsy
pERK 1/2Thr202/Tyr204 in skeletal muscle tissue measured by immunoblot
时间窗: 24 hours
measurement of pERK 1/2Thr202/Tyr204 in skeletal muscle tissue taken by biopsy
pS6Ser235/236 in skeletal muscle tissue measured by immunoblot
时间窗: 24 hours
measurement of pS6Ser235/236 in skeletal muscle tissue taken by biopsy
p4EBP1Thr37/46 in skeletal muscle tissue measured by immunoblot
时间窗: 24 hours
measurement of p4EBP1Thr37/46 in skeletal muscle tissue taken by biopsy
ACC (acetyl-coenzyme A carboxylase) phosphorylation in skeletal muscle tissue measured by immunoblot
时间窗: 24 hours
ACC phosphorylation in skeletal muscle tissue taken by biopsy
pAMPKThr172 in skeletal muscle tissue measured by immunoblot
时间窗: 24 hours
measurement of pAMPKThr172 in skeletal muscle tissue taken by biopsy
IGF-1ea (insulin like growth factor) mRNA in skeletal muscle tissue measured by Quantitative polymerase chain reaction (qPCR)
时间窗: 24 hoursn
IGF-1ea mRNA in skeletal muscle tissue taken by biopsy
MGF (mechanical growth factor) mRNA in skeletal muscle tissue measured by Quantitative polymerase chain reaction (qPCR)
时间窗: 24 hours
MGF mRNA in skeletal muscle tissue taken by biopsy
STARS mRNA in skeletal muscle tissue measured by Quantitative polymerase chain reaction (qPCR)
时间窗: 24 hoursn
STARS mRNA in skeletal muscle tissue taken by biopsy
Myogenin mRNA in skeletal muscle tissue measured by Quantitative polymerase chain reaction (qPCR)
时间窗: 24 hours
Myogenin mRNA in skeletal muscle tissue taken by biopsy
IL-6 (interleukin 6) mRNA in skeletal muscle tissue measured by Quantitative polymerase chain reaction (qPCR)
时间窗: 24 hours
IL-6 mRNA in skeletal muscle tissue taken by biopsy
PGC1a4 (Peroxisome proliferator-activated receptor gamma coactivator 1-alpha 4) mRNA in skeletal muscle tissue measured by Quantitative polymerase chain reaction (qPCR)
时间窗: 24 hours
PGC1a4 mRNA in skeletal muscle tissue taken by biopsy
PGC1a (Peroxisome proliferator-activated receptor gamma coactivator 1-alpha) mRNA in skeletal muscle tissue measured by Quantitative polymerase chain reaction (qPCR)
时间窗: 24 hours
PGC1a mRNA in skeletal muscle tissue taken by biopsy
Atrogin mRNA in skeletal muscle tissue measured by Quantitative polymerase chain reaction (qPCR)
时间窗: 24 hours
Atrogin mRNA in skeletal muscle tissue taken by biopsy
Beclin mRNA in skeletal muscle tissue measured by Quantitative polymerase chain reaction (qPCR)
时间窗: 24 hours
Beclin mRNA in skeletal muscle tissue taken by biopsy
Myostatin mRNA in skeletal muscle tissue measured by Quantitative polymerase chain reaction (qPCR)
时间窗: 24 hours
Myostatin mRNA in skeletal muscle tissue taken by biopsy
次要结局
未报告次要终点
研究者
Antonio Paoli
Full Professor
University of Padova