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An Open Label Study of Multiple Doses of Cannabidiol in the Prevention of Acute Graft-Versus-Host Disease (GVHD)

Phase 2
Conditions
Prevention aGVHD
Interventions
Registration Number
NCT03840512
Lead Sponsor
Kalytera Therapeutics Israel, Ltd.
Brief Summary

A prospective, open-label, phase 2a study, to evaluate the pharmacokinetic (PK) profile, safety, and efficacy of multiple doses of Cannabidiol (CBD) in participants Graft-Versus-Host Disease (GVHD) after allogeneic hematopoietic stem cell transplantation (HSCT)

Detailed Description

The study contains 3 cohorts of 12 participants each: All participants will be orally administered for 105 days with CBD at doses of 75, 150 or 300 mg (PO) BID for the prevention of acute GVHD (aGVHD) following allogeneic HSCT.

In addition to the study drug, all participants will receive standard aGVHD prophylaxis consisting of a calcineurin inhibitor (cyclosporine or tacrolimus) and a short course of methotrexate (MTX). After completion of 105 treatment days, the participant will be followed-up until day 180.

Recruitment & Eligibility

Status
UNKNOWN
Sex
All
Target Recruitment
36
Inclusion Criteria
  1. Any malignant hematological disease in CR or Myelodysplastic Syndrome (MDS)
  2. Age ≥ 18 years
  3. Karnofsky Score (KS) ≥ 60%
  4. HSCT-Comorbidity Index (HSCT-CI) score ≤ 3
  5. No major organ dysfunction
  6. Myeloablative or reduced intensity conditioning regimen
  7. Matched (7/8 or 8/8) unrelated donor
  8. Peripheral blood stem cell graft
  9. Female subjects of childbearing potential must use a medically accepted method of contraception and must agree to continue use of this method for the duration of the study and for the follow-up time period. Acceptable methods of contraception include abstinence, barrier method with spermicide, intrauterine device (IUD), or steroidal contraceptive (oral, transdermal, implanted, and injected) in conjunction with a barrier method.
  10. Male subjects with partners of childbearing potential must agree to use adequate contraception (barrier method or abstinence) during the study.
  11. Subject's written informed consent
Exclusion Criteria
  1. Malignant hematological disease other than MDS, not in CR
  2. Myelofibrosis
  3. Allogeneic transplantation from a matched or mismatched sibling donor
  4. Cord blood transplantation
  5. Positive serology for HIV
  6. Serious psychiatric or psychological disorders
  7. Any uncontrolled infection at time of registration
  8. Active consumption of illicit drugs (such as: Crack cocaine, Heroin, Methamphetamines, Cocaine, Bath Salts, Amphetamines, Methadone, Benzodiazepine, Ecstasy)
  9. Use of Cannabis and/or its derivatives fourteen days prior to HSCT and for the duration of study participation
  10. Uncontrolled hepatitis B or active hepatitis C infection.
  11. QTc>450ms per Fridericia's correction and Impaired cardiac function or clinically significant cardiac diseases
  12. Inadequate renal function defined as measured creatinine clearance > 2.0 mg/dl
  13. Liver enzymes: ALT and AST > 3x upper limit of normal
  14. Pregnancy or breastfeeding ((positive serum β-HCG 7 days before first dose)
  15. Treatment with another investigational drug, biological agent, or device within 30 days of first dose, or investigational cell therapy within 6 months of first dose

Study & Design

Study Type
INTERVENTIONAL
Study Design
SEQUENTIAL
Arm && Interventions
GroupInterventionDescription
Oral CBD 150 BIDCBD-
Oral CBD 75 BIDCBD-
Oral CBD 300 BIDCBD-
Primary Outcome Measures
NameTimeMethod
Adverse Events (AEs) and serious adverse events (SAEs) ReportingUp to day 180

All AEs will be recorded, whether considered minor or serious, drug-related or not

Cumulative incidence of aGVHD at day 100 post-transplantFirst 100 days after transplant

Cumulative Incidence of Grade B-D aGvHD

Pharmacokinetic parameters of Cannabidiol (CBD) - TlagBlood samples will be obtained on Day 7 before HSCT and Day 7 post HSCT. Sampling times: immediately after CBD dosing and at 15, 30, 45, 60, 120, 180, and 240 minutes, and 8, 12 and 24 hours after dosing of CBD

Pharmacokinetic (PK) profile - Tlag - Absorption lag-time defined as the time of the first concentration ≥ Limit of Quantitation (LOQ)

Pharmacokinetic parameters of Cannabidiol (CBD) - CmaxBlood samples will be obtained on Day 7 before HSCT and Day 7 post HSCT. Sampling times: immediately after CBD dosing and at 15, 30, 45, 60, 120, 180, and 240 minutes, and 8, 12 and 24 hours after dosing of CBD

Pharmacokinetic (PK) profile - Cmax - Maximum Plasma Concentration

Pharmacokinetic parameters of Cannabidiol (CBD) - TmaxBlood samples will be obtained on Day 7 before HSCT and Day 7 post HSCT. Sampling times: immediately after CBD dosing and at 15, 30, 45, 60, 120, 180, and 240 minutes, and 8, 12 and 24 hours after dosing of CBD

Pharmacokinetic (PK) profile - Tmax - time to reach maximum plasma concentration

Pharmacokinetic parameters of Cannabidiol (CBD) - AUC0-tBlood samples will be obtained on Day 7 before HSCT and Day 7 post HSCT. Sampling times: immediately after CBD dosing and at 15, 30, 45, 60, 120, 180, and 240 minutes, and 8, 12 and 24 hours after dosing of CBD

Pharmacokinetic (PK) profile - AUC0-t - area under the plasma concentration-time curve (AUC0-t) up to the last quantifiable concentration (LOQ) from time of administration (t=0) up to the selected

Pharmacokinetic parameters of Cannabidiol (CBD) - λzBlood samples will be obtained on Day 7 before HSCT and Day 7 post HSCT. Sampling times: immediately after CBD dosing and at 15, 30, 45, 60, 120, 180, and 240 minutes, and 8, 12 and 24 hours after dosing of CBD

Pharmacokinetic (PK) profile: λz - Elimination rate constant determined by linear regression of the terminal points of the ln-linear plasma concentration-time curve

Pharmacokinetic parameters of Cannabidiol (CBD) - T1/2Blood samples will be obtained on Day 7 before HSCT and Day 7 post HSCT. Sampling times: immediately after CBD dosing and at 15, 30, 45, 60, 120, 180, and 240 minutes, and 8, 12 and 24 hours after dosing of CBD

Pharmacokinetic (PK) profile: T1/2 - Terminal elimination half-life

Pharmacokinetic parameters of Cannabidiol (CBD) - AUC0-∞Blood samples will be obtained on Day 7 before HSCT and Day 7 post HSCT. Sampling times: immediately after CBD dosing and at 15, 30, 45, 60, 120, 180, and 240 minutes, and 8, 12 and 24 hours after dosing of CBD

Pharmacokinetic (PK) profile: AUC0-∞ - area under the plasma concentration-time curve extrapolated to infinity

Cumulative incidence of aGVHD at day 180 post-transplantDay 180 post-transplant

Cumulative Incidence of Grade 2-4 aGvHD

Secondary Outcome Measures
NameTimeMethod

Trial Locations

Locations (5)

St Vincent's Hospital Sydney - The Kinghorn Cancer Centre

🇦🇺

Sydney, Australia

Davidof Cancer Center, Beilinson hospital, Rabin medical center

🇮🇱

Petach Tikva, Israel

Hadassah Medical Center - Bone Marrow Transplantation Department, Cancer Immunotherapy and Immunobiology Research Center

🇮🇱

Jerusalem, Israel

Rambam Health Care - Bone Marrow Transplantation Unit

🇮🇱

Haifa, Israel

Tel-Aviv Sourasky Medical Center - Bone Marrow Transplantation Unit

🇮🇱

Tel Aviv, Israel

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