Muscle Training Induced Angiogenesis in COPD

Not Applicable

Completed

• Conditions: Peripheral Muscle Dysfunction; COPD
• Interventions: Procedure: Muscular biopsy; Other: 5-10 weeks sport training

• Registration Number: NCT02040363
• Lead Sponsor: University Hospital, Montpellier

Brief Summary

COPD patients experiment a peripheral muscle dysfunction which impact their exercise tolerance and health-related quality of life. The capillary to fiber interface is reduced and impact the exercise capacity of the patients. While the muscle capillary creation in response to exercise training is blunted in COPD patients, the maturation of the neo-capillaries could also be blunted and contribute to the impaired aniogionenesis in patients. Because the capillary maturation is a sensitive and dynamic process, only different modalities of exercise training and multiple time-points of measures would allow to capture this microvascular adaptation.

Aim of the study : Compare the muscle capillary maturation in response to training at 5 and 10 weeks, in sedentary healthy subject trained at the intensity of the ventilatory threshold (60-65% of VO2max), versus :- COPD patients trained at a similar intensity (60-65% of VO2max)- COPD patients trained at a similar absolute intensity (90% of VO2max).

Detailed Description

Not available

Study Timeline

• Study Start: 2014-01
• Study First Submitted: 2014-01-10
• Study First Submitted QC: 2014-01-16
• Study First Posted: 2014-01-20
• Primary Completion: 2016-04
• Study Completion: 2016-08
• Status Verified: 2020-01
• Last Update Submitted: 2020-01-10
• Last Update Posted: 2020-01-14

Recruitment & Eligibility

• Status: COMPLETED
• Sex: All
• Target Recruitment: 22

Inclusion Criteria

• COPD patients :
• FEV1<80%pred.
• non exacerbation during the past 4 weeks
• no oxygen therapy, -

Healthy subjects:

• <150min/week of moderate-to-vigorous physical activity,
• Voorrips score <9

Exclusion Criteria

• Unstable comorbidity
• Long term oxygen therapy or ventilation
• Rehabilitation program during the past 12 months
• nutritional supplementation during the past 4 weeks
• allopurinol or N-acetylcystein during the past 4 weeks
• long corticosteroid treatment during the past 6 months
• hyperreactivity to xylocain
• anticoagulant or platelet aggregation inhibitors treatment
• participation to an other research study-pregnancy or impossibility to provide informed consent, ...

Study & Design

• Study Type: INTERVENTIONAL
• Study Design: PARALLEL

Arm && Interventions

Group	Intervention	Description
healthy volunteers	5-10 weeks sport training	healthy volunteers
COPD patients ~60-65% VO2max	Muscular biopsy	COPD patients \~60-65% VO2max
healthy volunteers	Muscular biopsy	healthy volunteers
COPD Patients ~90% VO2max	Muscular biopsy	COPD Patients \~90% VO2max

Primary Outcome Measures

Name	Time	Method
Capillary-o-fiber ratio assessed by immunochemistry on a muscle biopsy of the vastus lateralis of the quadriceps	10 weeks

Secondary Outcome Measures

Name	Time	Method
Pericyte coverage of skeletal muscles capillaries assessed by immunostaining of a muscle biopsy of the vastus lateralis of the quadriceps	10 weeks
quadriceps endurance	10 weeks
Effect of the serum of COPD patients and SHS on pericyte/endothelial cells interactions in vitro	10 weeks
Effect of the serum of COPD patients and SHS on pericyte morphology and in vitro	10 weeks
Effect of the serum of COPD patients and SHS on pericyte/endothelial cells interactions in vivo	10 weeks

Trial Locations

Locations (1)

Department of clinical Physiology - University Hospital; 🇫🇷 Montpellier, France