Impact of Exercise Training on Skeletal Muscle Capillary Maturation During Angiogenesis in COPD Patients
Overview
- Phase
- Not Applicable
- Intervention
- Not specified
- Conditions
- Peripheral Muscle Dysfunction
- Sponsor
- University Hospital, Montpellier
- Enrollment
- 22
- Locations
- 1
- Primary Endpoint
- Capillary-o-fiber ratio assessed by immunochemistry on a muscle biopsy of the vastus lateralis of the quadriceps
- Status
- Completed
- Last Updated
- 6 years ago
Overview
Brief Summary
COPD patients experiment a peripheral muscle dysfunction which impact their exercise tolerance and health-related quality of life. The capillary to fiber interface is reduced and impact the exercise capacity of the patients. While the muscle capillary creation in response to exercise training is blunted in COPD patients, the maturation of the neo-capillaries could also be blunted and contribute to the impaired aniogionenesis in patients. Because the capillary maturation is a sensitive and dynamic process, only different modalities of exercise training and multiple time-points of measures would allow to capture this microvascular adaptation.
Aim of the study : Compare the muscle capillary maturation in response to training at 5 and 10 weeks, in sedentary healthy subject trained at the intensity of the ventilatory threshold (60-65% of VO2max), versus :- COPD patients trained at a similar intensity (60-65% of VO2max)- COPD patients trained at a similar absolute intensity (90% of VO2max).
Investigators
Eligibility Criteria
Inclusion Criteria
- •COPD patients :
- •FEV1\<80%pred.
- •non exacerbation during the past 4 weeks
- •no oxygen therapy, -
- •Healthy subjects:
- •\<150min/week of moderate-to-vigorous physical activity,
- •Voorrips score \<9
Exclusion Criteria
- •Unstable comorbidity
- •Long term oxygen therapy or ventilation
- •Rehabilitation program during the past 12 months
- •nutritional supplementation during the past 4 weeks
- •allopurinol or N-acetylcystein during the past 4 weeks
- •long corticosteroid treatment during the past 6 months
- •hyperreactivity to xylocain
- •anticoagulant or platelet aggregation inhibitors treatment
- •participation to an other research study-pregnancy or impossibility to provide informed consent, ...
Outcomes
Primary Outcomes
Capillary-o-fiber ratio assessed by immunochemistry on a muscle biopsy of the vastus lateralis of the quadriceps
Time Frame: 10 weeks
Secondary Outcomes
- Pericyte coverage of skeletal muscles capillaries assessed by immunostaining of a muscle biopsy of the vastus lateralis of the quadriceps(10 weeks)
- quadriceps endurance(10 weeks)
- Effect of the serum of COPD patients and SHS on pericyte/endothelial cells interactions in vitro(10 weeks)
- Effect of the serum of COPD patients and SHS on pericyte morphology and in vitro(10 weeks)
- Effect of the serum of COPD patients and SHS on pericyte/endothelial cells interactions in vivo(10 weeks)