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Circulating Tumor DNA Study in Patients With Endometrial Cancer

Recruiting
Conditions
Endometrial Cancer
Interventions
Other: Whole blood
Registration Number
NCT05955079
Lead Sponsor
Assistance Publique - H么pitaux de Paris
Brief Summary

The objective of this study is to identify a population at risk of early recurrence after oncologic resection surgery of a primary uterine tumor based on the detection of ctDNA

Detailed Description

Despite early management, the risk of recurrence in non-metastatic endometrial cancer (FIGO I-III) is approximately 10-20%. The challenge is to identify the most high-risk cases for relapse in order to adapt surgical and medical management.

The development of digital PCR methods in nano-droplets, detecting circulating tumor DNA (ctDNA) with high sensitivity, could help to better specify the prognosis of patients with localized endometrial cancer and to identify a population with residual disease, the source of this ctDNA.

The investigators established a universal methylation signature in the laboratory based on analysis of endometrial cancer-specific DNA methylation using in silico analysis of public data from the Cancer Genome Atlas, validated in an independent cohort, with 99% sensitivity and 98% specificity.

A prospective biological cohort was established between the gynecology and medical oncology departments and the Cochin Hospital biological resources center (CARPEM-OncoCentre collection).

This is a prospective monocentric biological collection study.

The aim of this study is to evaluate the prognostic impact of pre- and post-operative ctDNA detection in stage I-III endometrial cancer.

Recruitment & Eligibility

Status
RECRUITING
Sex
Female
Target Recruitment
130
Inclusion Criteria
  • Female patients over 18 years of age who are potentially eligible for inclusion in the OncoCentre collection (registered as a patient at APHP, without legal protection measures, affiliated with a social security system)
  • Patients diagnosed with histologically documented endometrial cancer on an endometrial biopsy
  • Surgical intervention performed at Hopital Cochin
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Exclusion Criteria
  • Failure to sign the OncoCentre consent form
  • Refusal of OncoCentre consent
  • Patient not eligible for upfront curative surgical treatment
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Study & Design

Study Type
OBSERVATIONAL
Study Design
Not specified
Arm && Interventions
GroupInterventionDescription
Endometrial CancerWhole bloodPatients over 18 years old with a biopsy-proven endometrial cancer, at FIGO stage I to IV, and amenable and undergoing surgical treatment
Primary Outcome Measures
NameTimeMethod
Recurrence-free survival1 year
Secondary Outcome Measures
NameTimeMethod
Frequency of ctDNA detection based on established prognostic parameters3 years

Frequency of ctDNA detection based on established prognostic parameters: histological type (endometrioid, non-endometrioid), grade (low grade, high grade), stage (localized to the uterus stages I-II or stage III with nodal involvement), lymphovascular invasion (present/absent), and molecular group (low risk: POLE, intermediate risk: MSI/NSMP, high risk: TP53).

Frequency of ctDNA detection based on the recurrence profile3 years

Frequency of ctDNA detection based on the recurrence profile : anatomical (locoregional versus distant; abdominal versus extra-abdominal; visceral or nodal) or dynamic (aggressive recurrence (progression-free survival post recurrence \<6 months) or non-aggressive (\>6 months))

Frequency of ctDNA detection in other prognostic groups3 years

Frequency of ctDNA detection in other prognostic groups according to the 2021 ESGO-ESTRO-ESP classification

Recurrence-free survival3 years

Trial Locations

Locations (1)

H么pital Cochin

馃嚝馃嚪

Paris, France

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