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Canola-Mediterranean Diet Study in T2DM

Not Applicable
Completed
Conditions
Overweight
Cardiovascular Diseases
Type 2 Diabetes
Obesity
Interventions
Behavioral: A canola oil enriched mediterranean diet
Behavioral: A high wheat fiber diet
Registration Number
NCT02245399
Lead Sponsor
Unity Health Toronto
Brief Summary

The purpose of the study is to assess whether a Mediterranean-type weight-loss diet, enriched with canola oil, high in plant protein, and low in carbohydrates will produce blood sugar control, reduce coronary heart disease (CHD) risk factors and maximize weight loss, better than conventional higher carbohydrate diets in overweight diabetic patients.

Detailed Description

The investigators plan to assess the effects of increasing both canola oil and plant protein foods while reducing carbohydrate intake in the context of a Mediterranean type diet on weight loss, glycemic control and cardiovascular risk factors in type 2 diabetes.

Obesity rates in Western nations have shown a dramatic rise in the last 20 years and diabetes rates have doubled, a trend which is predicted to be repeated over the next 20 years. In Canada the predicted cost to the healthcare system in only 7 years will rise to $17 billion. Weight loss diets (such as Atkins, Eddies, South Beach and Zone) emphasizing carbohydrate restriction have become increasingly popular for the prevention and treatment of diabetes. As a result, lower carbohydrate diets are being selected by health conscious members of the general population including those with diabetes. Because such diets in effect promote a high intake of protein from animal sources, even in the presence of weight loss, serum cholesterol levels rise due to increased cholesterol and saturated fat intake; and a further rise in serum lipids is likely to occur in the long term when weight loss has ceased. On the other hand, lower carbohydrate dietary patterns that are higher in plant rather than animal fat and proteins have been associated with improved blood lipids and reduced risk of heart disease and type 2 diabetes.

The investigators have therefore planned a study in which a weight reducing low carbohydrate, Mediterranean type diet that is high in plant proteins and canola oil will be compared to a weight reducing high cereal fibre diet in a 3 month study. 150 overweight and obese participants with type 2 diabetes will be randomized to one of 2 treatments.

Study visits will be as follows: weeks -2, 0 (for randomization), 2, 4, 8, 10 and 12. Body measurements, blood pressure and blood samples will be taken at each visit except week 2. The week 2 visit will be mainly for reinforcement of dietary advice; also body weight will be measured and blood drawn for fast glucose and HbA1c. Diet records will be reviewed at all visits. 24 hour urine samples will be collected at week 0 and week 12.

On completion of the 12 week study, participants will be given the option of continuing on the same diet or trying the opposite diet for a further 12 weeks. Visits will be every 4 weeks for a total of 3 visit. Body measurements, blood pressure and blood samples will be taken during these visits as in the initial 12 week study.

Recruitment & Eligibility

Status
COMPLETED
Sex
All
Target Recruitment
164
Inclusion Criteria
  • Men and women with type 2 diabetes diagnosed for more than 6 months
  • BMI >27 (non-Asians); BMI >25 (Asians)
  • HbA1c between 6.5% and 8.5% at screening, and at the preparation visit before starting diet
  • on a stable prescribed dose of oral diabetes medication for at least 2 months
  • on a stable dose of lipid medication for at least 2 weeks, if prescribed
  • on a stable dose of blood pressure medication for at least 1 week, if prescribed
  • have a family physician
  • can keep written food records, with the use of a digital scale
Exclusion Criteria

Individuals with the following characteristics/conditions will be excluded

  • on insulin
  • on steroids
  • on warfarin (Coumadin)
  • GI disease (gastroparesis, celiac, colitis, Crohn's disease, Inflammatory Bowel Syndrome)
  • history of cancer, except non-melanoma skin cancer (basal cell, squamous cell)
  • major cardiovascular event (stroke, myocardial infarction) in past 6 months
  • major surgery in past 6 months
  • major debilitating disorder
  • liver disease (AST or ALT> 3x the upper limit of normal) except non-alcoholic fatty liver (NAFL) disease or non-alcoholic steatohepatitis (NASH).
  • hepatitis B or C
  • renal failure (creatinine > 150 mmol/L)
  • serum triglycerides >4.5mmol/L
  • acute or chronic infections (bacterial or viral)
  • chronic inflammatory diseases (e.g. lupus, ulcerative colitis)
  • blood pressure >145/90, unless approved by their family physician
  • alcohol consumption >2 drinks/d
  • food allergies to wheat, canola, or other study food components
  • any condition determined by the investigators to make the subject unsuitable for the study

Study & Design

Study Type
INTERVENTIONAL
Study Design
PARALLEL
Arm && Interventions
GroupInterventionDescription
A canola oil enriched mediterranean dietA canola oil enriched mediterranean dietParticipants will be advised to consume, a low-carbohydrate diet (26-32% of calories), high in vegetable protein (28-32%) and fat (41-45%) with canola as the major component (10%). Carbohydrate sources will feature viscous fiber-containing foods (including psyllium cereal, oats and barley) and low-starch vegetables (emphasizing okra and eggplant) for the relatively limited amount of carbohydrate.
A high wheat fiber dietA high wheat fiber dietParticipant will be advised to consume a high carbohydrate diet (58% carbohydrate, 16% protein and 25% fat) emphasizing whole wheat/whole grain cereals and increased high fiber alternatives, with fruits and vegetables.
Primary Outcome Measures
NameTimeMethod
change in HbA1cMeasured at weeks -2, 0, and then at weeks 8, 10 and 12

The baseline HbA1c will be the average HbA1c of weeks -2 and week 0. The end HbA1c will be the average HbA1c of weeks 8, 10 and 12. Change will be the difference between the end and baseline values

Secondary Outcome Measures
NameTimeMethod
24-hour Ambulatory blood pressure profileAt weeks -1 and 12

Measurement will be done using a non-invasive SpaceLabs 90217 Ambulatory BP monitor

diet historyAt weeks 0, 2, 4, 8, 10 and 12

7-day food records brought in at weeks 0, 2, 4, 8, 10 and 12 will be analyzed for macro and micro nutrient intakes.

C-reactive proteinAt weeks 0 and 12
Serum lipids: total cholesterol, LDL-chol, HDL-chol and TriglyceridesAt weeks 0, 4, 8, 10 and 12
urinary analysesweek 0 and week 12

24 hr urine samples will be analyzed for creatinine, urea, C-peptide, minerals, electrolytes and other dietary biomarkers

Blood pressureAt weeks 0, 4, 8, 10 and 12
blood glucoseAt weeks 0, 2, 4, 8, 10 and 12
Cholesterol absorptionAt weeks -1 and 12

This is an optional sub-study that will be carried out prior to the first and last weeks of the 12 week study. On day 1 (a Monday) of the week -1 and last weeks of the study after an overnight 12hr fast, blood will be drawn and participants will be asked to ingest 75mg of the stable carbon isotope \[3, 4-13C\] cholesterol dissolved in 5g of margarine and spread on half of an English muffin. Subsequent blood draws will be taken after an overnight 12hr fast, at 48 h (a Wednesday) and 72 h (a Thursday) after ingestion of the labeled cholesterol.

Change in body weightbaseline (week 0) and end (week 12)
change in LDL particle sizeWeeks 0 and 12

LDL particle size at weeks 0 and 12 will be determined by assessing their electrophoretic characteristics obtained by non-denaturing polyacrylamide gradient (2-16%) gel electrophoresis of serum

Trial Locations

Locations (3)

Richardson Centre for Functional Foods and Nutraceuticals, University of Manitoba

🇨🇦

Winnipeg, Manitoba, Canada

Risk Factor Modification Centre, St. Michael's Hospital

🇨🇦

Toronto, Ontario, Canada

Institute of Nutraceuticals and Functional Foods, Laval University

🇨🇦

Quebec City, Quebec, Canada

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