Effect of Abstinence Duration on Embryo Development: a Prospective Sibling Oocyte Study
试验速览
- 阶段
- 不适用
- 状态
- 已完成
- 入组人数
- 100
- 试验地点
- 1
- 主要终点
- Embryo development (blastocyst stage)
研究概览
简要总结
The aim the investigators study is to analyze the embryo development if oocytes of the participants are injected with sperm from a longer or shorter (2 hours) abstinence duration.
详细描述
Participants undergoing an IVF treatment, are asked to respect an abstinence duration of 2-7 days. This is to obtain a sufficient number of motile sperm. A recent investigation, analyzed the quality (number and motility) of a sperm sample produced after an abstinence duration of 4 to 7 days, followed by a second sperm sample 2 hours later. The second sample (after 2 hours) was able to produce sperm cells with a higher motility. Not only has this short abstinence duration of 2 hours a positive effect on the motility, it will also reduce the sperm DNA damage, which again has a positive effect on embryo development. Because participants need gametes of high quality to obtain embryos with a high quality, the investigators want to investigate what the effect is of abstinence duration on embryo development.
研究设计
- 研究类型
- Interventional
- 分配方式
- Randomized
- 干预模型
- Parallel
- 主要目的
- Diagnostic
- 盲法
- None
入排标准
- 年龄范围
- 18 Years 至 —（Adult, Older Adult）
- 性别
- All
- 接受健康志愿者
- 是
入选标准
- •Patients planned for embryo development up to day 5
- •at least 6 mature oocytes
- •fresh ejaculates
- •fresh embryo transfer or freeze all strategy
- •all stimulation protocols
- •concentration of >1x10^6 /ml sperm
排除标准
- •IVF versus ICSI patients
- •chirurgical sperm
- •acceptors of donor oocytes
- •patients with PGD
- •patients already enrolled in another study
研究组 & 干预措施
'normal' abstinence duration
Patients follow the normal abstinence duration as asked by the physician (2-7 days). This sperm will be used to inject half of the oocytes Intervention = second sperm sample
干预措施: second masturbation (Other)
short abstinence duration
Patients will be asked to produce a second sperm sample, 2 hours after the first. This sperm will be used to inject the other half of the oocytes.
Intervention = second sperm sample
干预措施: second masturbation (Other)
结局指标
主要结局
Embryo development (blastocyst stage)
时间窗: 1 year
embryo development 5/6 of the preimplantation development
Embryo development (cleavage stage)
时间窗: 1 year
embryo development on day 3 of the preimplantation development
次要结局
- Clinical Pregnancies(1 year)
- Utilization rate(1 year)
- Sperm DNA fragmentation(1 year)
- Sperm quality(1 year)
- Fertilization(1 year)
研究者
Neelke De Munck
Clinical Embryologist
Universitair Ziekenhuis Brussel