Effect of Exercise on Irisin Levels of Hypothyroid Rats

Not Applicable

Completed

• Conditions: Hypothyroidism

• Registration Number: NCT06699069
• Lead Sponsor: Riphah International University

Brief Summary

The aim of study was to evaluate the role of exercise on serum irisin levels in hypothyroid rats.

Detailed Description

Contracting skeletal muscle acts as an endocrine organ, capable of secreting hormones called myokines, such as interleukin 1, Interleukin 15 and Irisin. Myokines are important for regulation of glucose and lipid metabolism. Among these, irisin, is an exercise induced polypeptide, secreted mainly by skeletal muscles and adipose tissue. Irisin is also secreted in small amounts from cardiac muscle, pancreas and sebaceous glands. It is cleaved and secreted from fibronectin type III domain-containing protein 5 (FNDC5 gene) and is regulated by the peroxisome proliferator-activated receptor-γ co activator 1-α (PGC1-α) .

The most important function of Irisin is thermogenesis, by inducing the browning of white adipose tissue, thereby increasing energy consumption, encouraging weight loss and decreasing dietary insulin resistance. Linking Irisin levels with obesity, its mechanism shows that after exercise there is up regulation of a thermogenin ,an Uncoupling Protein-1 (UCP-1) Thyroid hormone also plays an important role in glucose-lipid metabolism and energy homeostasis. Hypothyroidism, a decrease in thyroid hormone levels, can lead to obesity and metabolic syndrome. It has been found that thyroid hormone is also involved in thermogenesis by inducing browning of white adipose tissue, through thyroid hormone receptors. In hypothyroidism, many metabolic processes in the body are affected such as differentiation in the adipose tissue. Increased thyroid stimulating hormone (TSH) causes adipogenesis by stimulating pre-adipocyte differentiation directly through the receptors in the adipose tissue . Fatty tissue is increased by adipocyte hypertrophy as a consequence of previous adipogenesis, and newly formed adipocyte-8, resulting in clinical obesity.

FNDC5 (Fibronectin type III domain-containing protein 5), which is the precursor of irisin, is a protein that is encoded by the FNDC5 gene, is present in many tissues, including the thyroid tissue and therefore effects thyroid hormone.

Dysregulation of both Thyroid and Irisin hormones result in metabolic syndrome. The metabolic syndrome is a common metabolic disorder that has been linked to the increasing prevalence of obesity. By regulating thyroid hormone levels, irisin has a powerful impact on metabolism and thermogenesis. The association of irisin with the thyroid hormone in hypothyroidism still remains unknown and is controversial, and requires further confirmatory studies on experimental models.

Regular exercise is known to have significant beneficial effects on the metabolic health and this is mediated by skeletal muscle. Contracting skeletal muscle is the source of Irisin. Studies have shown an increase in post exercise Irisin levels

Basic Information
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Recruitment & Eligibility
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Study & Design
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Trial Locations

Study Timeline

• Study First Submitted: 2021-02-02
• Study Start: 2021-02-20
• Primary Completion: 2022-01-30
• Study Completion: 2022-01-30
• Status Verified: 2024-11
• Study First Submitted QC: 2024-11-19
• Last Update Submitted: 2024-11-19
• Study First Posted: 2024-11-21
• Last Update Posted: 2024-11-21

Recruitment & Eligibility

• Status: COMPLETED
• Sex: Male
• Target Recruitment: 30

Inclusion Criteria

• Male adult rats weighing approx. 200-250gms
• 6 to 8 week old rats

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Exclusion Criteria

• Female rats will be excluded.
• Rats with any obvious pathology
• The rats weighing >200gm

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Study & Design

• Study Type: INTERVENTIONAL
• Study Design: PARALLEL

Primary Outcome Measures

Name	Time	Method
Serum Triiodothyronine T3	11th week	Estimation of serum free triiodothyronine (FT3) levels (ng/ml) by radioimmunoassay
Serum Thyroxine	11th week	Estimation of serum free thyroxin (FT4) levels (μg/dl) by radioimmunoassay
Serum Irisin	11th week	Estimation of serum irisin levels (μg/ml) by using enzyme - linked immunosorbent assay (ELISA) using irisin competitive ELISA Kit
Serum Thyroid Stimulating Hormone	11th week	By radioimmunoassay
Serum Triglycerides (TG)	11th week	By enzymatic method using micro lab analyzer
Serum High Density Lipoproteins (HDL)	11th week	Determined by precipitating method using micro lab analyzer
Serum Low Density Lipoproteins (LDL)	11th week	LDL= Total cholesterol-HDL-TG/5

Trial Locations

Locations (1)

National Institute of Health; 🇵🇰 Islamabad, Federal, Pakistan