Effects of Taurine Supplementation Associated With High Intensity Physical Training on Irisin Levels, Energy Expenditure and Body Composition of Obese Women
试验速览
- 阶段
- 不适用
- 状态
- 已完成
- 入组人数
- 31
- 试验地点
- 1
- 主要终点
- Changes in plasma taurine concentration
研究概览
简要总结
Irisin is a myocin secreted by skeletal muscle in stimulus to physical exercise and has been described as a possible therapeutic tool in the fight against obesity by triggering a cascade of signaling that triggers the expression of genes responsible for the increase in energy expenditure and browning of adipose tissue white cells by activating thermogenesis promoter mitochondrial proteins. Although the science is engaged in the search for evidences about the actions of the irisin, the physical exercise involved and the improvement in the levels of obesity, many mechanisms are still unknown. Since taurine by means of irisin-like pathways also increases energy expenditure, it is believed that taurine supplementation associated with high intensity aerobic physical exercise can optimize the effects of irisin, increasing energy expenditure and improving body composition in obesity.
详细描述
Introduction
Irisin is a myocin secreted by skeletal muscle when stimulated by physical exercise that promotes increased thermogenesis (browning effect). Similarly, taurine is also associated with modulations of energy metabolism with promising actions in the control of metabolic disorders. Thus, it is believed that chronic taurine supplementation associated with physical training can optimize irisin concentrations after exercise.
Objective
Evaluate the effects of taurine supplementation associated with interval aerobic training on plasma irisin concentrations, energy expenditure, body composition and physical fitness in obese women. Methods: Twenty-two women aged 25 to 45 years, sedentary, with grade I obesity (BMI ≥30-35 kg / m²) and without comorbidities will be recruited for the study. The subjects will be submited to a high intensity interval aerobic training for 8 weeks, being 3 times by week, for 50 minutes, associated to the supplementation with 3g of taurine or placebo.
Methods: Assessment of dietary intake, Resting Energy Expenditure (GER) by indirect calorimetry, body composition by deuterium oxide, anthropometric measurements, HPLC plasma analysis of taurine, multiplex irisin and physical fitness variables will be performed pre and post intervention. The results will be expressed as mean and standard deviation and the ANOVA test two way repeated measures mixed model will be applied, with post hoc Sidak, to verify differences and statistical interactions (p <0.05).
研究设计
- 研究类型
- Interventional
- 分配方式
- Randomized
- 干预模型
- Parallel
- 主要目的
- Other
- 盲法
- Double (Participant, Investigator)
盲法说明
Intervention with taurine and / or placebo supplementation and physical training
入排标准
- 年龄范围
- 25 Years 至 45 Years（Adult）
- 性别
- Female
- 接受健康志愿者
- 否
入选标准
- •This study included women with obesity grade I (BMI ≥30 - ≤35Kg/m²), sedentary, with no associated comorbidity, convenience sample.
排除标准
- •Women classified as overweight, obesity grade II and grade III (morbid), who present comorbidities associated with obesity (pre-diabetes, diabetes, hypertension, dyslipidemia, cardiovascular diseases and diseases of bone metabolism); smokers; who use hormones, sports supplements and / or weight loss medications. Perform some type of nutritional monitoring followed by diet or weight loss guidelines and that have medical impediment to the practice of physical exercise.
结局指标
主要结局
Changes in plasma taurine concentration
时间窗: Two times: (1) First day and (2) 10 weeks after assessments, adaptation and intervention
Collected in EDTA tubes, were centrifuged and the plasma taurine concentration was evaluated using HPLC (high performance liquid chromatography).
Changes Physical Performance
时间窗: Two times: (1) First day and (2) 10 weeks after assessments, adaptation and intervention
Maximum effort test, according to Wilder, Brennan e Schotte (1993).
Changes body composition
时间窗: Two times: (1) First day and (2) 10 weeks after assessments, adaptation and intervention
The body composition was evaluated through deuterium oxide
Changes in total cholesterol and lipid fractions
时间窗: Two times: (1) First day and (2) 10 weeks after assessments, adaptation and intervention
Collected in EDTA tubes, were centrifuged.
Changes in plasma irisin concentration
时间窗: Two times: (1) First day and (2) 10 weeks after assessments, adaptation and intervention
Collected in EDTA tubes, were centrifuged and the plasma irisin concentration was evaluated using Multiplex - Kit Human Myokine Magnetic Bead Panel.
Changes indirect calorimetry
时间窗: Two times: (1) First day and (2) 10 weeks after assessments, adaptation and intervention
With a gas analyzer (indirect calorimeter), we evaluated the metabolic rate and rest (REE) and oxidation of substrates (lipids and carbohydrates)
Changes in determination of lactate
时间窗: Two times: (1) First day and (2) 10 weeks after assessments, adaptation and intervention
Blood samples were collected by manual puncture of the earlobe in previously calibrated and heparinized capillary tubes, stored in eppendorf with sodium fluoride. Analyzed by electrochemical lactate analyser.
Changes body weight
时间窗: Two times: (1) First day and (2) 10 weeks after assessments, adaptation and intervention
The body weight was measured by digital balance before and after the intervention
Changes Food intake
时间窗: Two times: (1) First day and (2) 10 weeks after assessments, adaptation and intervention
Food registry of 3 days. The quantification of the daily intake of nutrients will still be made using sofware.
次要结局
未报告次要终点
研究者
Ellen Cristini de Freitas
Principal Investigator
University of Sao Paulo