Taurine Supplementation and Physical Training Effects on Adipose Tissue Mitochondrial Energy Metabolism, and Blood Inflammation and Oxidative Stress in Obese Women
试验速览
- 阶段
- 不适用
- 状态
- 已完成
- 入组人数
- 24
- 试验地点
- 2
- 主要终点
- Changes from baseline in interleukines levels at 8 weeks
研究概览
简要总结
Taurine supplementation researches have increased due to its antioxidant and anti-inflammatory actions, and its ability to modulate lipid metabolism by stimulating the expression of proteins that regulates mitochondrial biogenesis and increases respiratory function (PGC-1α and PPAR) and irisin release when associated to exercise. Since obesity can induce metabolic disorders including abnormal production of adipokines and activation of pro-inflammatory signaling pathways also mitochondrial metabolism dysfunction in the adipose tissue, the use of taurine would be a new strategy for obesity prevention and treatment. Moreover, the association of taurine and exercise could improve exercise effects, promote higher energy expenditure and increase mitochondrial respiration, consequently resulting in weight loss. Therefore, the present investigation aims to evaluate the effects of the association of taurine supplementation and a combined exercise training protocol (aerobic and strength) on resting energy expenditure, weight, body composition, blood markers of inflammation and oxidative stress, telomeres length, and mitochondrial function and the expression of genes that regulates energy metabolism and lipid oxidation in the white adipose tissue in obese women.
详细描述
A double-blind placebo-controlled study was conducted with 24 obese women (32.9±6.3 years). Capsules of taurine (3 grams) (GTau) or placebo (GP) were daily supplemented 2 hours before training. The training program was composed of aerobic and strength exercises during one hour, 3 times a week, for an 8-week period (intensity of 80% heart rate). The taurine supplemented group received only taurine capsules (3g/day) during 8 weeks. Measurement of weight, hip and waist circumference, and body composition (by Deuterium oxide) were performed before and after the intervention. Resting energy expenditure and nutrients oxidation were assessed by calorimetry.
In order to check the effects of the intervention, abdominal tissue biopsy will be performed for white adipose tissue analysis, evaluation of mitochondrial function and quantification of the expression of genes related to energy metabolism and lipid oxidation and taurine pathway; blood collection will be done for quantification of taurine levels, inflammatory (IL-10, IL-15, IL-6, IL-1, TNF-α, and CRP), adipokines (adiponectin, adipsin, resistin, fetuin and leptin) and oxidative stress (GPx, SOD and MDA) markers. Also, evaluation of telomere length was performed. Body composition was evaluated by deuterium oxide method, weight, waist and hip circumference were accessed. All the measurements were performed before and after the intervention period.
研究设计
- 研究类型
- Interventional
- 分配方式
- Randomized
- 干预模型
- Parallel
- 主要目的
- Treatment
- 盲法
- Double (Participant, Investigator)
盲法说明
Supplement capsule packages were labeled as "Supplement A" and "Supplement B" and the nutrient (taurine or placebo- starch) was revealed after the end of the intervention.
入排标准
- 年龄范围
- 20 Years 至 45 Years（Adult）
- 性别
- Female
- 接受健康志愿者
- 是
入选标准
- •Body Mass Index of 30 to 40 kg / m²
- •Sedentary
- •No associated co morbidity
排除标准
- •Women who have a medical impediment to the practice of physical exercise
- •Women that have undergone bariatric surgery
- •Menopause, cancer or any metabolic disease
- •Alcoholics
- •Insulin-dependent diabetes
结局指标
主要结局
Changes from baseline in interleukines levels at 8 weeks
时间窗: eight weeks
Change of inflammatory markers such as interleukines 6, 10 and 15 were calculated at 8 weeks in comparision to the baseline.
Change from baseline in white adipose tissue mitochondrial respiration at 8 weeks
时间窗: eight weeks
A subcutaneous adipose tissue sample collected for analysis of mitochondrial respiration (mitochondrial uncoupled state, phosphorylation state and electron transport system maximal capacity) were calculated at 8 weeks in comparison to the baseline.
Changes from baseline in macronutrient intake at 8 weeks
时间窗: eight weeks
Change of macronutrient intake were calculated at 8 weeks in comparision to the baseline.
Changes from baseline in body composition at 8 weeks
时间窗: eight weeks
Change of body composition through deuterium oxide method were calculated at 8 weeks in comparision to the baseline.
Changes from baseline in superoxide dismutase levels at 8 weeks
时间窗: eight weeks
Change of oxidative stress markers such as superoxide dismutase were calculated at 8 weeks in comparision to the baseline.
Changes from baseline in total calorie intake at 8 weeks
时间窗: eight weeks
Change of total calorie intake were calculated at 8 weeks in comparision to the baseline.
Change from baseline in indirect calorimetry at 8 weeks
时间窗: eight weeks
Change of energy expenditure and lipids oxidation were calculated at 8 weeks in comparision to the baseline.
Changes from baseline in cytokine levels at 8 weeks
时间窗: eight weeks
Change of inflammatory markers such as adiponectin, resistin and adipsin were calculated at 8 weeks in comparision to the baseline.
Changes from baseline in glutathione peroxidase levels at 8 weeks
时间窗: eight weeks
Change of oxidative stress markers such as glutathione peroxidase were calculated at 8 weeks in comparision to the baseline.
次要结局
未报告次要终点
研究者
Ellen Cristini de Freitas
Principal Investigator
University of Sao Paulo