Taurine Supplementation and Training Effects on Energy Metabolism, Inflammation and Oxidative Stress in Obese Women
- Conditions
- Obesity
- Interventions
- Dietary Supplement: PlaceboDietary Supplement: TaurineOther: Exercise training
- Registration Number
- NCT04279600
- Lead Sponsor
- University of Sao Paulo
- Brief Summary
Taurine supplementation researches have increased due to its antioxidant and anti-inflammatory actions, and its ability to modulate lipid metabolism by stimulating the expression of proteins that regulates mitochondrial biogenesis and increases respiratory function (PGC-1α and PPAR) and irisin release when associated to exercise. Since obesity can induce metabolic disorders including abnormal production of adipokines and activation of pro-inflammatory signaling pathways also mitochondrial metabolism dysfunction in the adipose tissue, the use of taurine would be a new strategy for obesity prevention and treatment. Moreover, the association of taurine and exercise could improve exercise effects, promote higher energy expenditure and increase mitochondrial respiration, consequently resulting in weight loss. Therefore, the present investigation aims to evaluate the effects of the association of taurine supplementation and a combined exercise training protocol (aerobic and strength) on resting energy expenditure, weight, body composition, blood markers of inflammation and oxidative stress, telomeres length, and mitochondrial function and the expression of genes that regulates energy metabolism and lipid oxidation in the white adipose tissue in obese women.
- Detailed Description
A double-blind placebo-controlled study was conducted with 24 obese women (32.9±6.3 years). Capsules of taurine (3 grams) (GTau) or placebo (GP) were daily supplemented 2 hours before training. The training program was composed of aerobic and strength exercises during one hour, 3 times a week, for an 8-week period (intensity of 80% heart rate). The taurine supplemented group received only taurine capsules (3g/day) during 8 weeks. Measurement of weight, hip and waist circumference, and body composition (by Deuterium oxide) were performed before and after the intervention. Resting energy expenditure and nutrients oxidation were assessed by calorimetry.
In order to check the effects of the intervention, abdominal tissue biopsy will be performed for white adipose tissue analysis, evaluation of mitochondrial function and quantification of the expression of genes related to energy metabolism and lipid oxidation and taurine pathway; blood collection will be done for quantification of taurine levels, inflammatory (IL-10, IL-15, IL-6, IL-1, TNF-α, and CRP), adipokines (adiponectin, adipsin, resistin, fetuin and leptin) and oxidative stress (GPx, SOD and MDA) markers. Also, evaluation of telomere length was performed. Body composition was evaluated by deuterium oxide method, weight, waist and hip circumference were accessed. All the measurements were performed before and after the intervention period.
Recruitment & Eligibility
- Status
- COMPLETED
- Sex
- Female
- Target Recruitment
- 24
- Body Mass Index of 30 to 40 kg / m²
- Sedentary
- No associated co morbidity
- Women who have a medical impediment to the practice of physical exercise
- Women that have undergone bariatric surgery
- Menopause, cancer or any metabolic disease
- Smokers
- Alcoholics
- Insulin-dependent diabetes
Study & Design
- Study Type
- INTERVENTIONAL
- Study Design
- PARALLEL
- Arm && Interventions
Group Intervention Description Taurine supplementation associated to exercise training Exercise training Taurine supplementation composed of capsules of taurine powder. Dosage: 3 grams/day Frequency: 1 time/day Duration: 8 weeks Exercise training Exercise Protocol: a combination of strength and aerobic exercises Duration: 2 weeks of adaptation and 8 weeks of physical training. Frequency: 3 times/week Duration: 55 minutes/session Intensity: 75 to 90% of maximum heart rate Placebo supplementation associated to exercise training Exercise training Placebo supplementation composed of capsules of starch powder. Dosage: 3 grams/day Frequency: 1 time/day Duration: 8 weeks Exercise training Exercise Protocol: a combination of strength and aerobic exercises Duration: 2 weeks of adaptation and 8 weeks of physical training. Frequency: 3 times/week Duration: 55 minutes/session Intensity: 75 to 90% of maximum heart rate Placebo supplementation associated to exercise training Placebo Placebo supplementation composed of capsules of starch powder. Dosage: 3 grams/day Frequency: 1 time/day Duration: 8 weeks Exercise training Exercise Protocol: a combination of strength and aerobic exercises Duration: 2 weeks of adaptation and 8 weeks of physical training. Frequency: 3 times/week Duration: 55 minutes/session Intensity: 75 to 90% of maximum heart rate Taurine supplementation Taurine Taurine supplementation composed of capsules of taurine powder. Dosage: 3 grams/day Frequency: 1 time/day Duration: 8 weeks Taurine supplementation associated to exercise training Taurine Taurine supplementation composed of capsules of taurine powder. Dosage: 3 grams/day Frequency: 1 time/day Duration: 8 weeks Exercise training Exercise Protocol: a combination of strength and aerobic exercises Duration: 2 weeks of adaptation and 8 weeks of physical training. Frequency: 3 times/week Duration: 55 minutes/session Intensity: 75 to 90% of maximum heart rate
- Primary Outcome Measures
Name Time Method Change from baseline in white adipose tissue mitochondrial respiration at 8 weeks eight weeks A subcutaneous adipose tissue sample collected for analysis of mitochondrial respiration (mitochondrial uncoupled state, phosphorylation state and electron transport system maximal capacity) were calculated at 8 weeks in comparison to the baseline.
Changes from baseline in macronutrient intake at 8 weeks eight weeks Change of macronutrient intake were calculated at 8 weeks in comparision to the baseline.
Changes from baseline in body composition at 8 weeks eight weeks Change of body composition through deuterium oxide method were calculated at 8 weeks in comparision to the baseline.
Changes from baseline in interleukines levels at 8 weeks eight weeks Change of inflammatory markers such as interleukines 6, 10 and 15 were calculated at 8 weeks in comparision to the baseline.
Changes from baseline in superoxide dismutase levels at 8 weeks eight weeks Change of oxidative stress markers such as superoxide dismutase were calculated at 8 weeks in comparision to the baseline.
Changes from baseline in total calorie intake at 8 weeks eight weeks Change of total calorie intake were calculated at 8 weeks in comparision to the baseline.
Change from baseline in indirect calorimetry at 8 weeks eight weeks Change of energy expenditure and lipids oxidation were calculated at 8 weeks in comparision to the baseline.
Changes from baseline in cytokine levels at 8 weeks eight weeks Change of inflammatory markers such as adiponectin, resistin and adipsin were calculated at 8 weeks in comparision to the baseline.
Changes from baseline in glutathione peroxidase levels at 8 weeks eight weeks Change of oxidative stress markers such as glutathione peroxidase were calculated at 8 weeks in comparision to the baseline.
- Secondary Outcome Measures
Name Time Method
Trial Locations
- Locations (1)
School of Physical Education and Sport of Ribeirão Preto
🇧🇷Ribeirão Preto, Sao Paulo, Brazil