The Acute Effect of Different Resistance Exercise Loads on Irisin and Brain-derived Neurotrophic Factor in Blood Serum and Plasma

Not Applicable

Recruiting

• Conditions: BDNF; Irisin; Resistance Exercise; Stroop Test; Lactate; Healthy Young Adult

• Registration Number: NCT07189130
• Lead Sponsor: University of Ljubljana

Brief Summary

The aim of this study is to investigate different resistance training loads on BNDF and irisin levels in platelets, blood serum and plasma.

Detailed Description

In this study, the investigators will examine how three resistance training protocols acutely affect BDNF levels (in serum, plasma, platelet count, and BDNF per platelet) and irisin levels in blood (plasma). The resistance training protocols will differ in intensity, rest intervals between sets and repetition execution. Blood samples will be collected from participants at four time points: before the start of the protocol, 5 minutes after completion of the final set, 1 hour after completion of the final set, and 3 hours after completion of the final set. In addition, blood lactate levels will be monitored at the same time points. Heart rate will also be recorded (immediately before the start of the protocol and 30 seconds after completion of the final set). A control protocol will also be implemented, in which blood will be collected at the same time points. Data will be collected across five visits to the kinesiology laboratory at the University of Ljubljana, Faculty of Sport. Each visit will last approximately 4 hours, except for the first visit where 1RM will be measured.

Study Timeline

• Study Start: 2025-05-12
• Study First Submitted: 2025-08-19
• Status Verified: 2025-09
• Study First Submitted QC: 2025-09-15
• Study First Posted: 2025-09-23
• Last Update Submitted: 2025-09-24
• Last Update Posted: 2025-09-30
• Primary Completion: 2025-10-30
• Study Completion: 2026-09-01

Recruitment & Eligibility

• Status: RECRUITING
• Sex: Male
• Target Recruitment: 24

Inclusion Criteria

• males between 18 and 30 years
• moderate level of physical activity assessed with IPAQ

Exclusion Criteria

• injuries
• cardiovascular diseases
• respiratory diseases
• psychiatric diseases
• metabolic diseases
• other neurodegenerative diseases

Study & Design

• Study Type: INTERVENTIONAL
• Study Design: SINGLE_GROUP

Primary Outcome Measures

Name	Time	Method
Irisin	pre, 5 minutes post exercise, 1 hour post exercise, 3 hours post exercise.	Irisin will be measured in blood plasma.
serum BDNF	pre, 5 minutes post exercise, 1 hour post exercise, 3 hours post exercise.	BDNF will be determined in blood serum with ELISA kit.
Platelet count	pre, 5 minutes post exercise, 1 hour post exercise, 3 hours post exercise.	Platelet count in blood will be done.
BDNF per platelet	pre, 5 minutes post exercise, 1 hour post exercise, 3 hours post exercise.	The amount of BDNF per platelet will be calculated.
plasma BDNF	pre, 5 minutes post exercise, 1 hour post exercise, 3 hours post exercise.	BDNF will be determined in blood plasma with ELISA kit.

Secondary Outcome Measures

Name	Time	Method
Lactate	pre, 5 minutes post exercise, 1 hour post exercise, 3 hours post exercise.	Lactate will be measured in blood.
Stroop test	pre, 15 minutes post exercise.	Stroop test will be used as an assessment of cognitive functions. The number of read word will be assessed.

Trial Locations

Locations (1)

University of Ljubljana, Faculty of Sport; 🇸🇮 Ljubljana, Slovenia