Chronic Presence of Epstein Barr Virus in Epithelial Cells From Gingiva is Associated With Periodontitis

Not Applicable

Completed

• Conditions: Presence / Absence of EBV; Chronic Periodontitis; Aggressive Periodontitis
• Interventions: Procedure: dental root planing

• Registration Number: NCT01246596
• Lead Sponsor: Centre Hospitalier Universitaire de Nice

Brief Summary

The etiopathogenesis of periodontal disease results from complex interaction between infectious agents, mainly including bacteria, and host cellular and humoral immune responses. However it is thought that bacteria-induced pathogenesis is not sufficient alone to explain all biological and clinical features of the destructive periodontal disease. The main hypothesis is that herpesviruses, such as Epstein-Barr Virus, may participate as well by altering epithelial gingival cell biology and consequently may promote the initiation and progression of periodontitis.

Detailed Description

Not available

Study Timeline

• Study First Submitted: 2010-11-17
• Study First Submitted QC: 2010-11-22
• Study First Posted: 2010-11-23
• Study Start: 2011-01
• Primary Completion: 2014-05-05
• Study Completion: 2014-05-05
• Status Verified: 2023-11
• Last Update Submitted: 2023-11-09
• Last Update Posted: 2023-11-13

Recruitment & Eligibility

• Status: COMPLETED
• Sex: All
• Target Recruitment: 23

Inclusion Criteria

• 10 patients affected by chronic periodontitis
• 10 patients affected by aggressive periodontitis
• 10 healthy patients (orthodontics extraction)
• Subject who have read and understood the information note relative under investigation and who have signed the informed consent
• major patient

Exclusion Criteria

• Patient with no contre-indication to periodontal treatment
• Patient with serious blood disease
• Patient who exhibits systemic condition incompatible with realization of the study
• Patient treated with oral retinoids, bisphosphonates, oral anticoagulants and anticonvulsivants
• Patient who presents a buccal carcinoma treated with radiotherapy
• Patient who have received during the past 6 months: anti-inflammatory drugs, anti-cancerous or immunosuppressive chemiotherapy
• Patient who presents a linguistic or psychic incapacity to understand information

Study & Design

• Study Type: INTERVENTIONAL
• Study Design: PARALLEL

Arm && Interventions

Group	Intervention	Description
chronic periodontitis	dental root planing	-
healthy patient (orthodontics extraction)	dental root planing	-
aggressive periodontitis	dental root planing	-

Primary Outcome Measures

Name	Time	Method
Percentage of gingival epithelial cells infected by EBV (by immunofluorescence and In situ Hybridization)	at time=0

Secondary Outcome Measures

Name	Time	Method
Epithelial tissue infected by EBV (by immunofluorescence and in situ hybridization)	at time = 0
Presence / absence of latency and / or lytic proteins (by immunofluorescence and in situ hybridization)	at time = 0

Trial Locations

Locations (1)

Dr Severine VINCENT; 🇫🇷 Nice, France