Chronic Presence of Epstein Barr Virus in Sulcular and Junctional Epithelial Tissue From Gingiva is Associated With Severe Periodontitis

Not Applicable

Completed

• Conditions: Parodontitis Aggressive; Parodontitis Chronic
• Interventions: Procedure: biopsy

• Registration Number: NCT01246648
• Lead Sponsor: Centre Hospitalier Universitaire de Nice

Brief Summary

The etiopathogenesis of periodontal disease results from complex interaction between infectious agents, mainly including bacteria, and host cellular and humoral immune responses. However it is thought that bacteria-induced pathogenesis is not sufficient alone to explain all biological and clinical features of the destructive periodontal disease. The main hypothesis is that herpesviruses, such as Epstein-Barr Virus, may participate as well by altering epithelial gingival cell biology and consequently may promote the initiation and progression of periodontitis.

Detailed Description

Not available

Study Timeline

• Study First Submitted: 2010-11-17
• Study First Submitted QC: 2010-11-22
• Study First Posted: 2010-11-23
• Study Start: 2011-01-17
• Primary Completion: 2015-04-01
• Study Completion: 2015-04-01
• Status Verified: 2024-06
• Last Update Submitted: 2024-06-26
• Last Update Posted: 2024-06-27

Recruitment & Eligibility

• Status: COMPLETED
• Sex: Male
• Target Recruitment: 6

Inclusion Criteria

• Men exclusively
• 12 years minimum
• 2 patients affected by chronic periodontitis
• 2 patients affected by aggressive periodontitis
• 2 healthy patients (orthodontics extraction)
• Subject who have read and understood the information note relative under investigation and who have signed the informed consent
• Subject whose legal representative signed the parental consent (if minor patient)

Exclusion Criteria

• Patient with contre-indication to dental extraction
• Patient witn serious blood disease
• Patient who exhibits systemic condition incompatible with the realization of the study
• Patient treated with oral retinoids, bisphosphonates, oral anticoagulants and anticonvulsivants
• Patient who presents za buccal carcinoma treated with radiotherapy
• Patient who have received during the past 6 months: anti-inflammatory drugs, anti-cancerous or immunosuppressive chemiotherapy
• Patient who presents a linguistic or psychic incapacity to understand information
• Female patient

Study & Design

• Study Type: INTERVENTIONAL
• Study Design: PARALLEL

Arm && Interventions

Group	Intervention	Description
agressive periodontitis	biopsy	-
chronic periodontitis	biopsy	-
healthy patients	biopsy	-

Primary Outcome Measures

Name	Time	Method
Presence/absence of EBV in the epithelial cells of periodontal pockets (by Immunofluorescence and in situ Hybridization	at time = 0 (baseline)

Secondary Outcome Measures

Name	Time	Method
- Epithelial tissue infected (by Immunofluorescence and In situ Hybridization) : detection of oral gingival epithelium, sulcular epithelium, junctional epithelium.	at time = 0 (baseline)
Presence/ absence of latency and/or lytic proteins (by immunofluorescence and in situ hybridization	at time = 0 (baseline)

Trial Locations

Locations (1)

CHU de Nice, Hôpital Saint Roch; 🇫🇷 Nice, France