Analysis of Proteome and Pathophysiological Characteristics of Human Blood Cells
Overview
- Phase
- Not Applicable
- Intervention
- Not specified
- Conditions
- Red Blood Cell
- Sponsor
- Heinrich-Heine University, Duesseldorf
- Enrollment
- 200
- Locations
- 1
- Primary Endpoint
- Blood examination
- Status
- Completed
- Last Updated
- 5 years ago
Overview
Brief Summary
The aim of this study is to investigate wether change in endothelial nitric oxid synthase of blood cells (erythrocytes and peripheral blood mononuclear cells (PBMC)) has an influence on development and progression of cardiovascular diseases.
Investigators
Klinik für Kardiologie, Pneumologie und Angiologie
Principle Investigator Prof. Dr. Malte Kelm
Heinrich-Heine University, Duesseldorf
Eligibility Criteria
Inclusion Criteria
- •healthy volunteers
- •Patients: stable coronary artery disease with accordingly endothelial dysfunction
Exclusion Criteria
- •acute inflammation (CRP\>0,5 mg/dl, leucocyte \>11000/dl)
- •malignant diseases
- •acute or terminated kidney disease
- •relevant heart failure (NYHA III-IV)
Outcomes
Primary Outcomes
Blood examination
Time Frame: baseline
Following parameters will be examined: * Blood count (red cells, white cells, platelets, hemoglobin, hematocrit, mean corpuscular volume (MCV), mean corpuscular hemoglobin (MCH), mean corpuscular hemoglobin concentration (MCHC), red blood cell distribution width (RDW), mean platelet volume (MPV) ) * Creatinin * Urea * Glucose * Glycated hemoglobin (HbA1c) * Cholesterol * Triglyceride * C-reactive Protein * Apolipoprotein
Secondary Outcomes
- Protein expression(baseline)
- Nitric oxid metabolite and proteins in blood plasma, red blood cells and peripheral blood mononuclear cells (PBMN)(baseline)
- Asymmetric dimethylarginine (ADMA) and Cytokine(baseline)
- Microparticles(baseline)
- Nitric oxid and reactive oxygen species(baseline)
- Immunocytochemistry(baseline)