Hormonal Balance in the Mobilization-Fatigue-Recovery Cycle: Dynamics of the Physiological and Psychological Response to Pre-Competitive Stress
试验速览
- 阶段
- 不适用
- 状态
- 已完成
- 发起方
- 入组人数
- 30
- 试验地点
- 1
- 主要终点
- Changes from baseline in cortisol level.
研究概览
简要总结
This observational study investigates how elite athletes respond physiologically and psychologically to pre-competitive stress in the context of a high-stakes performance test. The study focuses on the dynamic changes in stress-related hormonal biomarkers and self-reported psychological states across three key phases: mobilization (pre-exercise anticipation), fatigue (immediate post-effort), and recovery (1 hour and 24 hours post-exercise). The performance test serves as part of a qualification process for national and international championship competitions, providing an ecologically valid model of competitive stress. Blood samples and validated psychological questionnaires are used to assess hormonal fluctuations and subjective stress responses at four time points.
The main question it aims to answer is:
How do key hormonal markers and self-reported psychological stress levels change across the mobilization-fatigue-recovery cycle surrounding a competitive performance?
详细描述
Elite athletes frequently experience intense psychological and physiological stress before and during high-stakes competitions. While a certain level of pre-competitive stress is necessary for optimal performance, excessive or poorly regulated stress can negatively affect both performance outcomes and recovery. The body's response to stress involves complex neuroendocrine mechanisms, including the activation of the hypothalamic-pituitary-adrenal (HPA) axis and the release of stress-related hormones and neuromodulators. These include cortisol, testosterone, dopamine, serotonin, β-endorphins, and endocannabinoids such as anandamide and 2-arachidonoylglycerol (2-AG).
This observational cohort study aims to explore the dynamic changes in both hormonal and psychological stress responses across four time points-before, immediately after, 1 hour after, and 24 hours after-a standardized, high-intensity rowing performance test. This test is part of the official national team selection process and simulates the psychological and physical demands of real-world competition, making it an ecologically valid model for investigating stress.
Participants are elite rowers (men and women) who are current members of the national team. The test represents a critical selection tool for major championship events (e.g., World and National Championships), creating a natural, high-pressure environment to study pre-competitive stress. All participants provide blood samples at four time points and complete validated psychological questionnaires (including CSAI-2 and SCAT) before the test.
The study's primary outcomes include blood concentrations of stress- and motivation-related hormones (cortisol, testosterone, dopamine, serotonin, β-endorphin, anandamide, 2-AG), and scores from standardized psychological stress inventories assessing cognitive anxiety, somatic anxiety, and self-confidence. These variables are evaluated across the mobilization-fatigue-recovery cycle to capture both immediate and delayed responses to competitive stress.
Secondary outcomes include anthropometric measurements (height, body weight, body mass index, body composition, and training history), which will be used to describe the participant cohort and assess potential moderating factors.
研究设计
- 研究类型
- Observational
- 观察模型
- Cohort
- 时间视角
- Retrospective
入排标准
- 年龄范围
- 19 Years 至 24 Years（Adult）
- 性别
- All
- 接受健康志愿者
- 是
入选标准
- •Aged 18-24 years
- •Member of the national rowing team
- •Actively participating in training and selection processes
- •Medically cleared for high-intensity exercise by a team physician
排除标准
- •Current injury or illness affecting physical performance
- •Use of medications that may influence hormonal or psychological responses (e.g., corticosteroids, antidepressants, hormonal therapies)
- •Diagnosed neurological, or endocrine disorders
- •Refusal to provide blood samples or complete questionnaires
结局指标
主要结局
Changes from baseline in cortisol level.
时间窗: At rest (before the test), directly after the test, and after 1-hour and 24-hour recovery periods.
Concentration of cortisol \[ng/ml\]. Immunoenzymatic assay method using a diagnostic ELISA Kit
Changes from baseline in testosterone level.
时间窗: At rest (before the test), directly after the test, and after 1-hour and 24-hour recovery periods.
Concentration of testosterone \[ng/mL\]. Immunoenzymatic assay method using a diagnostic ELISA Kit
Changes from baseline in serotonin level.
时间窗: At rest (before the test), directly after the test, and after 1-hour and 24-hour recovery periods.
Concentration of serotonin \[ng/mL\]. Immunoenzymatic assay method using a diagnostic ELISA Kit
Changes from baseline in dopamine level.
时间窗: At rest (before the test), directly after the test, and after 1-hour and 24-hour recovery periods.
Concentration of dopamine \[ng/mL\]. Immunoenzymatic assay method using a diagnostic ELISA Kit
Changes from baseline in beta-endorphin level.
时间窗: At rest (before the test), directly after the test, and after 1-hour and 24-hour recovery periods.
Concentration of beta-endorphin \[ng/mL\]. Immunoenzymatic assay method using a diagnostic ELISA Kit
Changes from baseline in anandamide level.
时间窗: At rest (before the test), directly after the test, and after 1-hour and 24-hour recovery periods.
Concentration of anandamide \[ng/mL\]. Immunoenzymatic assay method using a diagnostic ELISA Kit
Changes from baseline in 2-arachidonoyl glycerol level.
时间窗: At rest (before the test), directly after the test, and after 1-hour and 24-hour recovery periods.
Concentration of 2-arachidonoyl glycerol \[ng/mL\]. Immunoenzymatic assay method using a diagnostic ELISA Kit
Competitive Anxiety Profile Before Exercise Test Using SCAT Questionnaire
时间窗: Before the 2000-meter exercise test (baseline, at rest)
The Sport Competition Anxiety Test (SCAT) measures trait anxiety levels related to competitive situations in athletes. The SCAT questionnaire provides a score ranging from 10 to 30 points. Higher scores indicate higher levels of competitive anxiety, which is considered a worse outcome in terms of psychological readiness for competition. Lower scores suggest lower levels of trait anxiety and better psychological preparedness.
Cognitive, Somatic Anxiety, and Confidence Before and After Exercise Using CSAI-2R Questionnaire
时间窗: Before the 2000-meter exercise test (baseline, at rest)
The Competitive State Anxiety Inventory-2 Revised (CSAI-2R) measures the intensity of cognitive anxiety, somatic anxiety, and self-confidence in athletes before and after competitive performance. Each subscale of the CSAI-2R provides a score ranging from 9 to 36 points. For Cognitive Anxiety and Somatic Anxiety, higher scores indicate higher levels of anxiety, which are considered a worse outcome regarding psychological readiness. For Self-Confidence, higher scores indicate greater confidence, which is considered a better outcome.
Coping Strategies for Sport Stress Using CICS Questionnaire
时间窗: Before the 2000-meter exercise test (baseline, at rest)
The Coping Inventory for Competitive Sport (CICS) assesses athletes' coping strategies in response to competition-related stress. It measures task-oriented, distraction-oriented, and disengagement-oriented coping styles. Each subscale of the CICS (Task-Oriented Coping, Distraction-Oriented Coping, and Disengagement-Oriented Coping) provides a score ranging from 5 to 25 points. For Task-Oriented Coping, higher scores indicate more significant use of effective, adaptive coping strategies, which is considered a better outcome. For Distraction-Oriented Coping and Disengagement-Oriented Coping, higher scores indicate more significant use of less effective or maladaptive coping strategies, which is considered a worse outcome.
次要结局
未报告次要终点