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Phase II Combination Stem Cell Therapy for the Treatment of Severe Leg Ischemia

Phase 2
Completed
Conditions
Critical Limb Ischemia
Severe Leg Ischemia
Peripheral Artery Disease
Peripheral Vascular Disease
Interventions
Biological: MESENDO
Biological: Placebo
Registration Number
NCT00721006
Lead Sponsor
TCA Cellular Therapy
Brief Summary

The purpose of this research study is to compare in patients with double-sided claudication if the transplant of a combination of stem cells obtained from the bone marrow of the same patient will contribute to the formation of new blood vessels in one of the severly diseased ischemic limbs(legs)versus the control limb that receives a placebo product.

Limb Ischemia (LI) is a severe obstruction of the arteries which seriously decrease blood flow to the extremities (mainly feet and legs) and has progressed to the point of severe pain and even skin ulcers or sores.

LI needs comprehensive treatment since the condition will not improve on its own. The overall goal of treatment is to reduce pain and increase blood flow to improve symptoms or save the leg and feet. In many cases, current options for treatment including medications, surgery or endovascular procedures have not been successful.

In the last few years, investigators have explored therapies aimed to increase blood flow to the ischemic vessel by transplanting cells that will promote the development of new vessels in the diseased leg.

The study hypothesis is based on the concept that the process of formation of new blood vessels is complex and requires the participation of several types of stem cells and growth factors. The lack of any of these components will produce vessels which are immature and unable to provide appropriated blood supply to the leg.

Patients eligible to participate in the this study are those suffering from double-sided claudication with poor circulation or severe leg blockages, which are not candidates for surgical procedures.

Once the mixture of stem cells is prepared and the patient's bone marrow is ready, cells will be transplanted into the calf muscle of one the the diseased legs while the other diseased leg will receive the placebo. Clinical study to evaluate and compare the efficacy of the stem cell transplant will be performed for six months post cell transplant.

Detailed Description

Not available

Recruitment & Eligibility

Status
COMPLETED
Sex
All
Target Recruitment
35
Inclusion Criteria
  • Males or females older than 18 years of age.
  • Limb ischemia with ABI of < 0.7 in the index lower extremity in two consecutive examinations done at least 1 week apart.
  • Limb ischemia with resting ischemic pain and/or claudication at 100 meters and/or non-healing ulcers.
  • Claudication
  • Patients not considered candidates for surgical or percutaneous revascularization, due to poor target vessels, inability to cross total occlusions, or a morbidity which precludes general anesthesia.
Exclusion Criteria
  • Inability to provide informed consent.
  • Previous angiogenic therapy.
  • Known sensitivity to gentamycin and/or amphotericin B.
  • Use or expected use of antineoplastic drugs.
  • Any illness, which might affect the patient's survival after enrollment in the protocol.
  • Any illness or significant laboratory abnormality, which in the investigator's judgment will interfere with the patient's ability to comply with the protocol, compromise the patient's safety, or interfere with the interpretation of the study results.
  • No evidence of acute infection
  • WBC > 15000.
  • WBC < 4000.
  • Serum Creatinine > 3.0 mg/dL in patients who are not in hemodialysis.
  • Pregnant women or women planning to become pregnant or unwilling to use appropriate birth control methods before and 2 months after cell infusion.
  • Recent myocardial infarction within 3 months prior to screening.

Study & Design

Study Type
INTERVENTIONAL
Study Design
PARALLEL
Arm && Interventions
GroupInterventionDescription
MESENDOMESENDOAll subjects will receive active treatment in a blinded fashion in the left or right lower limb. The opposite lower limb will receive placebo.
placeboPlaceboAll subjects will receive placebo injections in a blinded fashion in the left or right lower limb. The opposite lower limb will receive active stem cell infusion
Primary Outcome Measures
NameTimeMethod
Enhancement of vessel formation accessed by Nuclear Perfusion Scan in critical limb ischemia.4 months
Secondary Outcome Measures
NameTimeMethod
Changes in resting leg pain identified by a Visual Analog Scale and patient safety4 months

Trial Locations

Locations (1)

TCA Cellular Therapy

🇺🇸

Covington, Louisiana, United States

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