Biphasic and Combined Effect of Aging & Obesity With Development of Effective Exercise Intervention Protocol
试验速览
- 阶段
- 不适用
- 状态
- 已完成
- 入组人数
- 28
- 试验地点
- 2
- 主要终点
- Single muscle fiber maximal force (Po)
研究概览
简要总结
Obesity leads to a vicious circle that increases intramuscular fat, insulin resistance, promotes muscular dysfunction resulting in increased muscle fat accumulation. The study of muscle function and intramyocellular lipids is insufficient for obesity. Particularly, the study of mechanisms in muscle function and intramyocellular lipids is few nationally and internationally.
详细描述
This study is prospective study. The goal of this study is
- To examine the distribution and contractile properties (cross-sectional area, maximal force, specific force, maximal shortening velocity) of single muscle fiber sex/Myosin heavy chain (MHC) type-related differences, by extracting a single muscle fiber from the vastus lateralis in young men and women.
- To examine the effects of intramyocellular lipids on physical activity ability, muscle dysfunction, and metabolic disorders in relation to aging and obesity.
- To examine the effects of intramyocellular lipids on physical activity ability, muscular dysfunction, and metabolic disorders after 12 weeks resistance exercise intervention.
研究设计
- 研究类型
- Interventional
- 分配方式
- Non Randomized
- 干预模型
- Parallel
- 主要目的
- Supportive Care
- 盲法
- Triple (Participant, Care Provider, Outcomes Assessor)
入排标准
- 年龄范围
- 20 Years 至 80 Years（Adult, Older Adult）
- 性别
- All
- 接受健康志愿者
- 是
入选标准
- •Young men: Age (20-35 yrs), BMI (19-23 Kg/m2)
- •Young women: Age (20-35 yrs), BMI (19-23 Kg/m2)
- •Elderly women: Age (65-80 yrs), BMI (19-23 Kg/m2)
- •Elderly obese women: Age (65-80 yrs), BMI ( >25 Kg/m2)
- •Subjects who can agree voluntarily.
排除标准
- •Acute coronary syndrome.
- •Uncontrolled hypertension.
- •Subjects who took drugs which can affects neuromuscular system.
- •Severely impaired cognition
- •Subjects who cannot agree voluntarily
结局指标
主要结局
Single muscle fiber maximal force (Po)
时间窗: Before intervention / after 12weeks intervention
Change from baseline Po to 12 weeks after initial assessment. Chemically skinned vastus lateralis muscle fiber Po (mN) is measured by slack test procedure. after measurement, Po is analyzed to compare the change after intervention from baseline.
Total droplet area
时间窗: Before intervention / after 12weeks intervention
Change from baseline total droplet area to 12 weeks after initial assessment. Total droplet area is determined by Oil-red-O staining. After staining, the total area (µm2) is analyzed to compare the change after intervention from baseline.
Number of lipid droplets
时间窗: Before intervention / after 12weeks intervention
Change from baseline number of lipid droplets to 12 weeks after initial assessment. Number of lipid droplets determined by Oil-red-O staining. After staining, number of lipid droplets is analyzed to compare the change after intervention from baseline.
Single muscle fiber specific force (SF)
时间窗: Before intervention / after 12weeks intervention
Change from baseline SF to 12 weeks after initial assessment. Chemically skinned vastus lateralis muscle fiber SF (kN/m2) is Po (kN) normalized by cross-sectional area (m2). SF is analyzed to compare the change after intervention from baseline.
Single muscle fiber MHC type distribution
时间窗: Before intervention / after 12weeks intervention
Change from baseline fiber type distribution to 12 weeks after initial assessment. The MHC composition of single fibers was determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). MHC type distribution is analyzed to compare the change after intervention from baseline.
Single muscle fiber cross-sectional area (CSA)
时间窗: Before intervention / after 12weeks intervention
Change from baseline CSA to 12 weeks after initial assessment. Chemically skinned vastus lateralis muscle fiber CSA (µm2) is measured by 3 dimensional microscopy. after analysis, CSA is compared the change after intervention from baseline.
Single muscle fiber maximal shortening velocity (Vo)
时间窗: Before intervention / after 12weeks intervention
Change from baseline Vo to 12 weeks after initial assessment. Chemically skinned vastus lateralis muscle fiber Vo (FL/s) is measured by slack test procedure. The time required to take up the imposed slack was measured from the onset of the length step to the beginning of the tension redevelopment . For each amplitude of length, the fiber was reextended while relaxed to minimize nonuniformity of sarcomere length. A straight line was presented to a plot of length vs. time, using least-squares regression, and the slope of the line divided by the segment length was recorded as Vo for that fiber. Vo is analyzed to compare the change after intervention from baseline.
次要结局
- Hand grip strength(Before intervention / after 12weeks intervention)
- Isokinetic knee extension strength(Before intervention / after 12weeks intervention)
研究者
Jae-Young Lim
Professor, Department of Rehabilitation Medicine
Seoul National University Bundang Hospital