Molecular Correlates of Methotrexate in Childhood ALL
试验速览
- 阶段
- 不适用
- 状态
- 已完成
- 入组人数
- 150
- 试验地点
- 1
- 主要终点
- Molecular basis for human reduced folate carrier (hRFC) transcripts
研究概览
简要总结
This laboratory study is looking at response or resistance to chemotherapy in young patients with acute lymphoblastic leukemia treated with methotrexate. Studying samples of tumor tissue in the laboratory from patients with cancer may help doctors learn more about changes that occur in DNA and drug resistance in patients.
详细描述
OBJECTIVES:
I. Determine the molecular basis for human reduced folate carrier (hRFC) transcripts in B-precursor and T-cell acute lymphoblastic leukemia (ALL) blasts obtained from children with newly diagnosed ALL subsequently treated with methotrexate.
II. Correlate hRFC expression in these specimens with methotrexate transport and sensitivities.
III. Determine the roles of high frequency gene/transcript variants for hRFC as determinants of response and resistance to methotrexate in these patients.
IV. Determine the roles of multidrug resistance-associated proteins as determinants of response and resistance to methotrexate and mercaptopurine in these patients.
研究设计
- 研究类型
- Observational
- 时间视角
- Prospective
入排标准
- 年龄范围
- — 至 17 Years（Child）
- 性别
- All
- 接受健康志愿者
- 否
入选标准
- •Diagnosis of B-precursor or T-cell acute lymphoblastic leukemia
- •Newly diagnosed disease subsequently treated with methotrexate
- •Banked diagnostic blast specimens are available from Childrens Oncology Group (COG) cellbank
排除标准
- 未提供
结局指标
主要结局
Molecular basis for human reduced folate carrier (hRFC) transcripts
时间窗: Up to 4 years
Correlation of hRFC expression and methotrexate transport and sensitivities
时间窗: Up to 4 years
High frequency gene/transcript variants for hRFC in relation to response and resistance to methotrexate
时间窗: Up to 4 years
Multidrug resistance-associated proteins in relation to response and resistance to methotrexate and mercaptopurine
时间窗: Up to 4 years
次要结局
未报告次要终点