Relationship Between the VEGF Gene Polymorphism and Cardiometabolic Risk Factors in People With Excessive Body Weight in the Polish Population
试验速览
- 阶段
- 不适用
- 状态
- 已完成
- 发起方
- 入组人数
- 400
- 试验地点
- 1
- 主要终点
- VEGF gene expression
研究概览
简要总结
The aim of the study is to determine the relationship between VEGF gene expression, VEGF gene polymorphism and serum leptin concentration in the Polish population in people with excessive body weight. In addition, the aim of the study is to look for relationships between the VEGF gene polymorphism and anthropometric and biochemical factors of cardiovascular risk and endothelial dysfunction such as body weight, waist circumference, serum total cholesterol, LDL, HDL, triglycerides, glucose and the occurrence of cardiovascular diseases in the family of a patient with excessive body weight in the Polish population.
详细描述
400 people (250 - study group; 150 control group) were subjected to subjective and objective action. Information on the occurrence of diseases and cardiovascular risk in the participant and his family was collected from research studies. Anthropometric parameters were measured (body weight, height, BMI, waist circumference, neck circumference) as well as blood pressure and pulse measurement. In addition, fasting venous blood was collected and secured. In venous blood currently marked with the following concentration: glucose, required cholesterol, LDL cholesterol, HDL cholesterol and triglycerides. In addition, a method of salting out and protecting DNA was developed from blood.
Furthermore the polymorphisms of VEGF genes (in positions: -2578 and -634) using the HMR (High Resolution Melt) method was determined.
Also VEGF and leptin by ELISA was determined. After obtaining the results of comparative analysis of the correlation between the occurrence of VEGF gene polymorphisms, serum levels of leptin and VEGF, and anthropometric and biochemical parameters of cardiovascular risk.
研究设计
- 研究类型
- Observational
- 观察模型
- Cohort
- 时间视角
- Other
入排标准
- 性别
- All
- 接受健康志愿者
- 是
入选标准
- •informed consent in writing
- •stable body mass (+/- 1 kg)
- •excessive body mass (BMI ≥ 25 kg/m2- study group)
- •proper body mass (BMI < 25 kg/m2- control group)
排除标准
- •age <18 years
- •secondary obesity
- •pregnancy, lactation
- •other conditions that in the opinion of researchers may pose any risk to the patient during the study.
结局指标
主要结局
VEGF gene expression
时间窗: through study completion, an average of 1 year
VEGF gene expression
VEGF gene polymorphism
时间窗: through study completion, an average of 1 year
VEGF gene polymorphism
serum leptin concentration
时间窗: through study completion, an average of 1 year
serum leptin concentration
次要结局
- Body mass (BM)(through study completion, an average of 1 year)
- Waist circumference (WC)(through study completion, an average of 1 year)
- low density lipoprotein (LDL)(through study completion, an average of 1 year)
- total cholesterol (TCH)(through study completion, an average of 1 year)
- high density lipoprotein (HDL)(through study completion, an average of 1 year)
- triglycerides(through study completion, an average of 1 year)
- glucose(through study completion, an average of 1 year)
研究者
Damian Skrypnik, MD, PhD
Principial Investigator
Poznan University of Medical Sciences