The effect of fasting-induced insulin resistance on brown adipose tissue activity, non-shivering thermogenesis and skeletal muscle mitochondrial uncoupling.
- Conditions
- Geenna
- Registration Number
- NL-OMON39127
- Lead Sponsor
- niversiteit Maastricht
- Brief Summary
Not available
- Detailed Description
Not available
Recruitment & Eligibility
- Status
- Pending
- Sex
- Not specified
- Target Recruitment
- 18
Healthy, lean (BMI 18-25 kg/m2) adults
Age: 18-30 years
Male and female
For females: use of a specific oral contraceptive (Microgynon 30 or levonorgestrel/ethinylestradiol)
Caucasian
Sedentary (=< times/week or =< 3 hours/week sports)
Cardiovascular diseases
Hypertension (systolic/diastolic blood pressure > 140/90)
Hypotension (systolic/diastolic blood pressure <90/60)
Use of beta-blockers
Asthma or other obstructive pulmonary diseases
Diabetes Mellitus type 1 and type 2
Elevated blood glucose levels (>5.6 mmol/l)
Hyperthyroidism
Pregnancy
Participation in earlier research or medical examinations that included PET/CT scanning
Radiation therapy due to medical treatment
Unstable body weight (weight gain or loss >3kg in the past 2 months)
Any known or suspected obstructive disease of the gastrointestinal tract, hypo motility disorders of the gastrointestinal tract or previous gastrointestinal surgery.
Study & Design
- Study Type
- Interventional
- Study Design
- Not specified
- Primary Outcome Measures
Name Time Method <p>The main study parameters are:<br /><br>- Standard uptake value (SUV) of BAT: This is used as a measure of BAT glucose<br /><br>uptake and BAT activity and will be measured by means of FDG-PET/CT scanning.<br /><br>- Skeletal muscle mitochondrial respiration/uncoupling: This will be determined<br /><br>in skeletal muscle samples using the Oroboros 2k Oxygraph instrument present in<br /><br>our laboratroy.<br /><br>- Energy expenditure: This will be measured by means of indirect calorimetry<br /><br>using a ventilated hood.<br /><br>- Ex vivo skeletal muscle glucose oxidation: This will be measured to determine<br /><br>insulin sensitivity. A glucose oxidation assay with insulin stimulation will be<br /><br>performed in skeletal muscle biopsies.</p><br>
- Secondary Outcome Measures
Name Time Method <p>Secondary study parameters include:<br /><br>-Body temperatures: Skin temperatures will be measured by means of iButtons and<br /><br>core temperature will be determined by ingestion of a telemetric pill.<br /><br>- Skin perfusion: This will be measured by means of LaserDoppler flowmetry.<br /><br>- Blood parameters: Venous blood will be drawn by means of a catheter placed in<br /><br>the antercubital vein of the underarm. By using radioimmunoassay and high<br /><br>performance liquid chromatography blood parameters will be analyzed.<br /><br>- UCP-1 and beta3-receptor polymorphims</p><br>