The Efficacy of a Non-Invasive 13CO2 'Breath Test' to Detect Anabolic Resistance Following Step Reduction in Younger and Older Adults
试验速览
- 阶段
- 不适用
- 状态
- 招募中
- 入组人数
- 32
- 试验地点
- 2
- 主要终点
- Net Leucine Retention (umol/kg)
研究概览
简要总结
Recent work in the investigators laboratory has examined the ability of a non-invasive 13CO2 breath-test to assess differences in amino acid oxidation rates and net balance in young healthy males following protein feeding and resistance exercise. The investigators aim to test the efficacy of this non-invasive 13CO2 breath-test to assess for differences in anabolic sensitivity between young and older adults following an acute period of habitual and reduced physical activity.
详细描述
Aging is associated with a phenomenon known as anabolic resistance, whereby individuals are less responsive to anabolic stimuli (i.e., resistance exercise, dietary protein ingestion). Mild and severe reductions in physical activity are also known to play a key role in the emergence of anabolic resistance. Therefore, the purpose of this study is to investigate the ability of a non-invasive 13CO2 breath-test to assess differences in whole-body leucine oxidation and leucine net balance between younger and older individuals under conditions of habitual activity and following a period of reduced activity/step-reduction. In this manner, the ability of the methodology to distinguish anabolic sensitivity between young and older adult populations will be assessed.
Methods:
Total participant time commitment will span over 4 sessional dates:
Session 1 - Introduction Interested participants will be recruited to engage in a video conference call to undergo an introductory session. Participants will be provided with a comprehensive introduction to the study which will explain the research objectives, conduct, and associated risks. Participants will provide informed consent prior to the collection of any study information (i.e., anthropometrics and habitual activity levels). Eligibility will also be screened for at this time.
Session 2 - Body Composition Assessment Participants will consume a bolus of deuterium oxide (D2O) and provide saliva samples in order to estimate fat-free mass.
研究设计
- 研究类型
- Interventional
- 分配方式
- Non Randomized
- 干预模型
- Parallel
- 主要目的
- Other
- 盲法
- None
入排标准
- 年龄范围
- 18 Years 至 80 Years（Adult, Older Adult）
- 性别
- All
- 接受健康志愿者
- 是
入选标准
- •Healthy young (age: 18-35 years) or older (age: 60-80 years) adults
- •Average daily step-count >7,000/day
- •BMI between normal to overweight (18.5-29.9 kg/m2)
排除标准
- •Regular use of nonsteroidal anti-inflammatory drugs (with the exception of daily low-dose aspirin)
- •Use of anticoagulants
- •Use of a walker, cane, or assistive walking device
- •Current or recently remised cancer
- •Infectious or gastrointestinal disease
- •Inability to comply with study protocol (e.g., >2,000 steps/day during Step-Reduction Phase)
- •Regular tobacco use
- •Self-reported illicit drug use (e.g. growth hormone, testosterone, etc.)
- •Diagnosed chronic illness (e.g. type 2 diabetes, heart disease, thyroid disease)
- •Hormonal Replacement Therapy
结局指标
主要结局
Net Leucine Retention (umol/kg)
时间窗: 6 hours
Whole-Body Net Leucine Retention determined from the difference between exogenous leucine oxidation and leucine ingestion the 6 hour measurement period.
Exogenous Leucine Oxidation (umol/kg)
时间窗: 6 hours
Exogenous Leucine Oxidation determined from breath 13CCO2 enrichment. Breath samples will be collected every 20-30min after test drink ingestion to determine breath 13CO2 enrichment. Total leucine oxidation will be determined from the area under the 13CO2 enrichment by time curve.
次要结局
未报告次要终点
研究者
Daniel Moore
Principal Investigator
University of Toronto