Treatment of Liver Metastases With Electrochemotherapy (ECTJ) Phase II

Phase 2

Completed

• Conditions: Liver Metastases
• Interventions: Procedure: Electrochemotherapy; Device: Cliniporator Vitae®; Drug: Bleomycin PHC 15 e. (United States Pharmacopeia - USP)

• Registration Number: NCT02352259
• Lead Sponsor: Institute of Oncology Ljubljana

Brief Summary

The study is prospective, phase II study, The primary objective of the study is evaluation of the feasibility and safety of intraoperative electrochemotherapy of colorectal liver metastases. The secondary objective is to determine the efficacy of electrochemotherapy treatment, based on histological and radiological evaluation of treated metastases. The endpoints are: toxicity according to the Common Terminology Criteria for Adverse Events (CTCAE) ver. 4.0 and response rate measured by percentage of vital tumor cells and mRECIST criteria.

Detailed Description

The study is an extension of previously concluded and published study on electrochemotherapy of colorectal liver metastases (Edhemovic et al. JSO 2014). In this extension 15 patients will be included. Four groups of patients will be included. The patients from groups 1 and 2 are patients with up to 3 unresectable liver metastases, demanding too excessive resection, or untreatable by standard thermal ablative methods due to the close proximity of major blood vessels. Electrochemotherapy will be offered to these patients as the only treatment option. In case of multiple, both resectable and non-resectable liver metastases, the latter will be treated with electrochemotherapy, whereas other metastases will be resected or treated by RFA. These groups of patients are:

1. Patients with one to three (1-3) metachronous liver metastases of colorectal cancer, not larger than 5 cm, that are positioned in unresectable liver area, near blood vessels, but in otherwise operable patients.

2. Recurrent liver metastases of colorectal cancer, not more than 3 and not larger than 5 cm in diameter, each. For those patients any other treatment could represent unacceptable risk due to possibility of insufficient liver residual after resection or less effective radiofrequency ablation due to the proximity of blood vessels.

Groups 3 and 4 include patients with intent to cure within standard of care using two-stage surgical approach. This two-stage surgical approach will allow adding electrochemotherapy during the first operation and tissue collection for histological analysis during the second operation. Adding electrochemotherapy to these patients will not affect their treatment within standard of care recommended in the current guidelines. These groups of patients are:

3. Patients with synchronous metastases, but their general condition and extent of the disease will not allow simultaneous removal of the primary tumor and metastases. During the first operation, the primary tumor will be removed (colorectal resection) and some of the liver metastases will be treated by electrochemotherapy. About 6 weeks later, during the second operation for liver metastases, both treated and non-treated metastases will be removed with liver resection.

4. Patients with bilateral, multiple, metachronous metastases in whom standard treatment includ two-stage liver resection, due to the extent of the disease and/or their general condition. During the first operation, right portal vein will be ligated and metastases on the left side will be excised or ablated with radiofrequency ablation. At the same time, up to three metastases on the right side were treated with electrochemotherapy. During the second operation, both treated and non-treated metastases on the right side will be removed with right hemihepatectomy.

Electrochemotherapy will be offered to the patients also when they refuse standard treatment.

Depending on the position of metastases, appropriate electrodes will be selected; hexagonal needle electrodes with fixed geometry for metastases not larger than 3 cm in diameter, where their lower edge is located up to 3 cm below the liver capsule. ECT will be performed based on standard operating procedures for treatment of cutaneous and subcutaneous tumors / metastases with ECT.

Individual electrodes, positioned according to the prepared treatment plan will be used for metastases up to 5 cm in diameter, or located near vena cava or large hepatic or portal veins.

Electrochemotherapy will be performed within 8-28 min after intravenous in bolus administration of bleomycin (15 mg/m2).

Triggering of electric pulses will be synchronized with ECG signals, through the ECG triggering device AccuSync to avoid delivery of pulses in vulnerable period of the heart.

All patients will be treated after the procedure has been thoroughly described to them, and have signed informed consent.

Study Timeline

• Study Start: 2013-09
• Study First Submitted: 2014-12-17
• Study First Submitted QC: 2015-01-27
• Study First Posted: 2015-02-02
• Primary Completion: 2018-10
• Study Completion: 2018-10
• Results First Submitted: 2020-11-12
• Status Verified: 2021-03
• Results First Submitted QC: 2021-03-01
• Last Update Submitted: 2021-03-01
• Results First Posted: 2021-03-24
• Last Update Posted: 2021-03-24

Recruitment & Eligibility

• Status: COMPLETED
• Sex: All
• Target Recruitment: 23

Inclusion Criteria

1. Histologically and/or cytologically confirmed colorectal cancer.
2. Age over 18.
3. Life expectancy more than 3 month.
4. Performance status Karnofsky ≥ 70 or (World Health Organization) WHO ≤ 2.
5. Chemotherapy free interval 2-5 weeks, depending on the drugs used.
6. Patient must be mentally capable of understanding the information given.
7. Patient must give informed consent.
8. Patient must be discussed at the multidisciplinary team for tumors of the gastrointestinal tract before entering the trial.

Exclusion Criteria

1. Secondary primary tumor, except surgically treated noninvasive cancer of cervix, or surgically or irradiated basal cell carcinoma
2. Visceral, bone or diffuse metastases.
3. Life-threatening infection and/or heart failure and/or liver failure and/or other severe systemic pathologies.
4. Clinically significant ascites.
5. Significant reduction in respiratory function.
6. Age less than 18 years.
7. Coagulation disturbances.
8. Cumulative dose of 250 mg/m2 bleomycin received.
9. Allergic reaction to bleomycin.
10. Impaired kidney function (creatinin > 150 µmol/l).
11. Patients with epilepsy.
12. Patients with arrhythmias.
13. Patients with pacemaker or defibrillator.
14. Pregnancy.
15. Patient incapable of understanding the aim of the study or disagree with the entering into the clinical study.

Study & Design

• Study Type: INTERVENTIONAL
• Study Design: SINGLE_GROUP

Arm && Interventions

Group	Intervention	Description
Electrochemotherapy	Bleomycin PHC 15 e. (United States Pharmacopeia - USP)	-
Electrochemotherapy	Cliniporator Vitae®	-
Electrochemotherapy	Electrochemotherapy	-

Primary Outcome Measures

Name	Time	Method
Evaluation of Safety Related to Electrochemotherapy	After operation on day 7	Recording of adverse events according to CTCAE criteria

Secondary Outcome Measures

Name	Time	Method
Response to Treatment (Determined by Modified RECIST Criteria).	After operation on the days 30, 60, 90 and 120	Response Evaluation Criteria In Solid Tumors Criteria (RECIST v1.0) for target lesions and assessed by MRI/CT: Complete Response (CR), Disappearance of all target lesions; Partial Response (PR), \>=30% decrease in the sum of the longest diameter of target lesions; Overall Response (OR) = CR + PR

Trial Locations

Locations (4)

Faculty of Electrical Engineering, University of Ljubljana, Slovenia; 🇸🇮 Ljubljana, Slovenia

Institute of Oncology Ljubljana; 🇸🇮 Ljubljana, Slovenia

University Medical Centre Ljubljana, Ljubljana, Slovenia; 🇸🇮 Ljubljana, Slovenia

University Medical Centre Maribor, Maribor, Slovenia; 🇸🇮 Maribor, Slovenia