Impact of Amino Acids to Enhance Anabolism After Home-based Exercise

Not Applicable

Completed

• Conditions: Exercise; Amino Acids, Essential; Metabolism

• Registration Number: NCT04850820
• Lead Sponsor: University of Toronto

Brief Summary

An adequate quantity of lean body mass is vital for optimal health and performance, and is accrued when net protein balance (NPB) is positive. Amino acids are the building blocks of protein, and when consumed following resistance exercise, significantly improve NPB.

However, no study has investigated how a supplement of all of the essential amino acids (EAA) affects NPB following resistance exercise in a free-living, home-based setting, particularly when compared to a supplement of branched-chain amino acids (BCAA).

Therefore, the purpose of this study is to determine the impact of a novel EAA supplement on anabolism (e.g., NPB) as compared to BCAA and placebo supplements following home-based resistance exercise.

Detailed Description

Not available

Study Timeline

• Study Start: 2021-04-01
• Study First Submitted: 2021-04-17
• Study First Submitted QC: 2021-04-17
• Study First Posted: 2021-04-20
• Primary Completion: 2021-12-31
• Study Completion: 2021-12-31
• Status Verified: 2022-04
• Last Update Submitted: 2022-04-29
• Last Update Posted: 2022-05-05

Recruitment & Eligibility

• Status: COMPLETED
• Sex: All
• Target Recruitment: 12

Inclusion Criteria

• Aged 18-35 years
• Recreationally active; currently performing structured exercise (e.g., running, weight lifting, team-sport activity) at least 2 days per week

Exclusion Criteria

• Body Mass Index: >29.9kg/m^2
• Inability to perform physical activity as determined by the Physical Activity Readiness Questionnaire
• Inability to adhere to protocol guidelines (e.g., alcohol, habitual diet)
• Regular tobacco use
• Illicit drug use (e.g., growth hormone, testosterone)
• Diagnosed medical condition under the care of a physician
• Inability to abstain from supplements (e.g., protein, creatine, HMB, BCAA, phosphatidic acid) at least three weeks before and during trials
• Individuals on any medications known to affect protein metabolism (e.g., corticosteroids, non-steroidal anti-inflammatories, or prescription-strength acne medications)

Study & Design

• Study Type: INTERVENTIONAL
• Study Design: CROSSOVER

Primary Outcome Measures

Name	Time	Method
Whole-body net leucine balance (umol/kg)	5 hours/trial (four trials total)	Used as a proxy of whole-body protein balance, and estimated by using \[13C\]leucine ingestion and breath/urine sample collection

Secondary Outcome Measures

Name	Time	Method
Urinary 3-methylhistidine (3-methylhistidine:creatinine ratio)	5 hours/trial (four trials total)	Used as a marker of muscle protein breakdown by measuring the 3-methylhistidine:creatinine ratio in the urine

Trial Locations

Locations (1)

Goldring Centre for High Performance Sport; 🇨🇦 Toronto, Ontario, Canada