Impact of Amino Acids to Enhance Anabolism After Home-based Exercise
- Conditions
- ExerciseAmino Acids, EssentialMetabolism
- Interventions
- Dietary Supplement: Novel essential amino acid supplementationDietary Supplement: Branched-chain amino acid supplementation (BCAA)Dietary Supplement: Isocaloric carbohydrate supplementation
- Registration Number
- NCT04850820
- Lead Sponsor
- University of Toronto
- Brief Summary
An adequate quantity of lean body mass is vital for optimal health and performance, and is accrued when net protein balance (NPB) is positive. Amino acids are the building blocks of protein, and when consumed following resistance exercise, significantly improve NPB.
However, no study has investigated how a supplement of all of the essential amino acids (EAA) affects NPB following resistance exercise in a free-living, home-based setting, particularly when compared to a supplement of branched-chain amino acids (BCAA).
Therefore, the purpose of this study is to determine the impact of a novel EAA supplement on anabolism (e.g., NPB) as compared to BCAA and placebo supplements following home-based resistance exercise.
- Detailed Description
Not available
Recruitment & Eligibility
- Status
- COMPLETED
- Sex
- All
- Target Recruitment
- 12
- Aged 18-35 years
- Recreationally active; currently performing structured exercise (e.g., running, weight lifting, team-sport activity) at least 2 days per week
- Body Mass Index: >29.9kg/m^2
- Inability to perform physical activity as determined by the Physical Activity Readiness Questionnaire
- Inability to adhere to protocol guidelines (e.g., alcohol, habitual diet)
- Regular tobacco use
- Illicit drug use (e.g., growth hormone, testosterone)
- Diagnosed medical condition under the care of a physician
- Inability to abstain from supplements (e.g., protein, creatine, HMB, BCAA, phosphatidic acid) at least three weeks before and during trials
- Individuals on any medications known to affect protein metabolism (e.g., corticosteroids, non-steroidal anti-inflammatories, or prescription-strength acne medications)
Study & Design
- Study Type
- INTERVENTIONAL
- Study Design
- CROSSOVER
- Arm && Interventions
Group Intervention Description Novel essential amino acid supplementation (EAA+) Novel essential amino acid supplementation Arm will investigate the effect of a novel essential amino acid-based formula (essential amino acids enriched with active botanical compounds; EAA+) developed by Iovate Health Sciences International Inc. on post-exercise anabolism. The formulation was recently granted a Natural Product Number (NPN: 80087022) and approved by the Natural and Non-prescription Health Products Directorate of Health Canada. Branched-chain amino acid supplementation (BCAA) Branched-chain amino acid supplementation (BCAA) Arm will used a branched-chain amino acid supplement developed by Iovate Health Sciences International Inc. to compare with the experimental arm after exercise. Rested control Isocaloric carbohydrate supplementation Carbohydrate supplement that is isocaloric to the EAA+ supplement but consumed at rest to serve as the baseline control Isocaloric carbohydrate supplementation Isocaloric carbohydrate supplementation Carbohydrate supplement that is isocaloric to the EAA+ supplement and designed to function as a placebo to the experimental arm after exercise.
- Primary Outcome Measures
Name Time Method Whole-body net leucine balance (umol/kg) 5 hours/trial (four trials total) Used as a proxy of whole-body protein balance, and estimated by using \[13C\]leucine ingestion and breath/urine sample collection
- Secondary Outcome Measures
Name Time Method Urinary 3-methylhistidine (3-methylhistidine:creatinine ratio) 5 hours/trial (four trials total) Used as a marker of muscle protein breakdown by measuring the 3-methylhistidine:creatinine ratio in the urine
Trial Locations
- Locations (1)
Goldring Centre for High Performance Sport
🇨🇦Toronto, Ontario, Canada