The Effect of Exercise and Presleep Nutrition on Muscle Connective Tissue Remodelling
Trial Snapshot
- Phase
- Not Applicable
- Status
- Recruiting
- Sponsor
- University of Toronto
- Enrollment
- 12
- Locations
- 1
- Primary Endpoint
- Enrichment of Muscle Collagen
Study Overview
Brief Summary
The primary objective of this study will be to determine the influence of a presleep vitamin C-enriched, collagen-modelled supplement (CVC) on the metabolic fate of dietary glycine using a [2H5]glycine tracer within the intramuscular connective tissue at rest and after a bout of resistance exercise. Other outcomes will be related to regulation collagen remodelling in skeletal muscle.
Study Design
- Study Type
- Interventional
- Allocation
- Randomized
- Intervention Model
- Crossover
- Primary Purpose
- Basic Science
- Masking
- None
Masking Description
Double blinded (investigators and participants). A lab member external to the investigation will code items for the intervention.
Eligibility Criteria
- Ages
- 18 Years to 35 Years (Adult)
- Sex
- All
- Accepts Healthy Volunteers
- Yes
Inclusion Criteria
- •Healthy, recreationally active, male and female participants.
- •Healthy will be defined as someone who does not have a health condition that would compromise their ability to safely participate in the strenuous physical activity involved in our study as screened for by the Get Active Questionnaire.
- •Willing to abstain from resistance exercise for 2 weeks prior to the first metabolic trial and for the duration of the study.
- •Participants must be able to abstain from lower-body resistance and/or plyometric exercise for at least 2 weeks prior to the first metabolic trial.
- •Participants will be aged 18-35 years old.
- •Participants must be normal to overweight (e.g., BMI 18.5-30).
- •Participants are willing to abide by the compliance rules of this study (e.g., abstain from physical activity and alcohol 48h prior to each trial).
- •Self-reported regular menstrual cycle (25-35d) within the last 3 months (female participants).
Exclusion Criteria
- •Inability to adhere to any of the compliance rules judged by principal investigator or medical doctor.
- •Self-reported regular tobacco use.
- •Self-reported illicit drug use (e.g. growth hormone, testosterone, etc.).
- •Regular use of anti-inflammatory drugs (e.g., ibuprofen).
- •Self-reported regularly sleeping less than an average of 7 hours over 3 days (minimum recommended daily amount).
- •Individuals who have participated in studies within the past year involving any of the stable isotopes in the study.
- •Use of birth control and discontinued use in the last 3-months (female only).
Arms & Interventions
Collagen and Vitamin C
Intervention: Collagen and Vitamin C (Dietary Supplement)
Carbohydrate Placebo
Intervention: Carbohydrate Placebo (Dietary Supplement)
Outcomes
Primary Outcomes
Enrichment of Muscle Collagen
Time Frame: 9 hours
Overnight incorporation of orally ingested amino acid tracer (D5 Gly and D5 Phe) into mature collagen protein isolated from skeletal muscle biopsy samples taken 9 hours after consumption of the tracer-containing supplement (CVC or PLA) and rest or resistance exercise. Enrichment will be quantified via tandem LC-MS/MS.
Secondary Outcomes
- Area of muscle with localized collagen remodelling(9 hours)
- Concentration of Urinary Growth Hormone(8 hours)
- Enrichment of Myofibrillar Protein(9 hours)
Investigators
Daniel Moore
Associate Professor
University of Toronto