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Metabolic Flexibility as a Biomarker of Adaptation to Diet and Exercise Challenges

Not Applicable
Completed
Conditions
Healthy Volunteers
Interventions
Other: Low Intensity Exercise
Other: High Intensity Exercise
Other: High Carbohydrate Beverage
Other: High Fat Beverage
Registration Number
NCT01987388
Lead Sponsor
USDA Beltsville Human Nutrition Research Center
Brief Summary

The objective is to develop a new metabolic flexibility biomarker, which has application in the study of changes diet and exercise on fuel management in humans. The new biomarker involves the characterization of an individual's metabolic flexibility utilizing room calorimeters rather than the current method, which is based on glucose clamp data. It is hypothesized that this new metabolic flexibility method will be a useful and noninvasive biomarker for measuring adaptation to exercise and diet challenges.

Detailed Description

Not available

Recruitment & Eligibility

Status
COMPLETED
Sex
All
Target Recruitment
16
Inclusion Criteria
  • Males and Females
  • Age 22 to 35 years at beginning of study
Exclusion Criteria
  • Women who have given birth during the previous 12 months
  • Pregnant women or women who plan to become pregnant or become pregnant during the study
  • Lactating women
  • History or presence of diabetes, kidney disease, liver disease, certain cancers, gastrointestinal, pancreatic, other metabolic diseases, or malabsorption syndromes.
  • History of bariatric or certain other surgeries related to weight control
  • Smokers or other tobacco users (for at least 6 months prior to the start of the study)
  • History of eating disorders or other dietary patterns which are not consistent with the dietary intervention (e.g., vegetarians, very low fat diets, high protein diets)
  • Volunteers who have lost 10% of body weight within the last 12 months or who plan to initiate a weight loss program during the next 10 months
  • Unable or unwilling to give informed consent or communicate with study staff
  • Self-report of alcohol or substance abuse within the past 12 months and/or current acute treatment or rehabilitation program for these problems (long-term participation in Alcoholics Anonymous is not an exclusion)
  • Other medical, psychiatric, or behavioral factors that in the judgment of the Principal Investigator may interfere with study participation or the ability to follow the intervention protocol
  • Inclusion and exclusion criteria have been established to recruit a population of individuals across a wide age-range that has typical digestive process

Study & Design

Study Type
INTERVENTIONAL
Study Design
CROSSOVER
Arm && Interventions
GroupInterventionDescription
Low Intensity Exercise, High Carbohydrate BeverageLow Intensity ExerciseStudy participant consumes a high carbohydrate beverage as part of the day's meals, and performs low intensity exercise (65% of VO2 max), while in the calorimeter.
Low Intensity Exercise, High Carbohydrate BeverageHigh Carbohydrate BeverageStudy participant consumes a high carbohydrate beverage as part of the day's meals, and performs low intensity exercise (65% of VO2 max), while in the calorimeter.
Low Intensity Exercise, High Fat BeverageHigh Fat BeverageStudy participant consumes a high fat beverage as part of the day's meals, and performs low intensity exercise (65% of VO2 max), while in the calorimeter.
High Intensity Exercise, High Carbohydrate BeverageHigh Intensity ExerciseStudy participant consumes a high carbohydrate beverage as part of the day's meals, and performs high intensity exercise (85% of VO2 max), while in the calorimeter.
High Intensity Exercise, High Carbohydrate BeverageHigh Carbohydrate BeverageStudy participant consumes a high carbohydrate beverage as part of the day's meals, and performs high intensity exercise (85% of VO2 max), while in the calorimeter.
High Intensity Exercise, High Fat BeverageHigh Intensity ExerciseStudy participant consumes a high fat beverage as part of the day's meals, and performs high intensity exercise (85% of VO2 max), while in the calorimeter.
High Intensity Exercise, High Fat BeverageHigh Fat BeverageStudy participant consumes a high fat beverage as part of the day's meals, and performs high intensity exercise (85% of VO2 max), while in the calorimeter.
Low Intensity Exercise, High Fat BeverageLow Intensity ExerciseStudy participant consumes a high fat beverage as part of the day's meals, and performs low intensity exercise (65% of VO2 max), while in the calorimeter.
Primary Outcome Measures
NameTimeMethod
Metabolic Flexibility by Room Calorimetry24 hours

Participants will stay in a room calorimeter for 24 hours where oxygen consumed and carbon dioxide produced will be measured. Mathematical approaches will use continuous calorimetry data to generate consistent estimates of the gas exchange rates, and hence accurate "instantaneous" respiratory exchange ratios. This novel approach allows evaluating the instantaneous dynamics of respiratory quotient noninvasively and for many different conditions (e.g., exercise and various nutrient ratios).

Secondary Outcome Measures
NameTimeMethod
Continuous Glucose Monitoring24 hours

Whole-day interstitial glucose profiles will be collected during each calorimeter measurement.

Trial Locations

Locations (1)

USDA Beltsville Human Nutrition Center

🇺🇸

Beltsville, Maryland, United States

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