Phage-Enabled Mining of Gut Metagenomes for Terpenoid and Carotenoid Metabolism
试验速览
- 阶段
- 不适用
- 状态
- 已完成
- 入组人数
- 40
- 试验地点
- 1
- 主要终点
- Number of unique gut metagenomic enzymes with carotenoid-modifying activities for each participant.
研究概览
简要总结
In this study, the investigators will collect fecal samples from healthy adults. The investigators will extract DNA from these fecal samples and use microbiological techniques to determine whether this DNA encodes enzymes that can react with certain chemicals called terpenoids and carotenoids. The investigators will additionally collect dietary information via a questionnaire.
研究设计
- 研究类型
- Observational
- 观察模型
- Cohort
- 时间视角
- Prospective
入排标准
- 年龄范围
- 18 Years 至 65 Years（Adult, Older Adult）
- 性别
- All
- 接受健康志愿者
- 是
入选标准
- •Body Mass index <25
排除标准
- •Taken antibiotics or antifungals in the past 3 months
- •Taken probiotic supplements in the past 3 months
- •Diarrhea in the past month
研究组 & 干预措施
Healthy adults
干预措施: No Intervention: Observational Cohort (Other)
结局指标
主要结局
Number of unique gut metagenomic enzymes with carotenoid-modifying activities for each participant.
时间窗: Day 1
Subjects' fecal metagenomic DNA will be extracted from their fecal sample, and random fragments of this metagenomic DNA will be inserted into an E. coli expression vector. This vector library will be transformed into an E. coli strain that will change color if a carotenoid-modifying gene is present. We expect to obtain about one million E. coli clones per participant. These clones will be spread on agar plates, and the colonies exhibiting different colors will be recovered and sequenced. The sequences of each participant will be collected and annotated to determine putative carotenoid-modifying enzymes, and the number of unique enzymes will be saved.
次要结局
未报告次要终点
研究者
Nathan Crook
Associate Professor
North Carolina State University