A Prospective study to determine gut microbiota composition in Indian children with history of antibiotic usage compared to those who did not use antibiotic by stool analysis
- Conditions
- Health Condition 1: A00-B99- Certain infectious and parasitic diseases
- Registration Number
- CTRI/2021/05/033685
- Lead Sponsor
- Dr Reddys Laboratories Ltd
- Brief Summary
Not available
- Detailed Description
Not available
Recruitment & Eligibility
- Status
- Completed
- Sex
- Not specified
- Target Recruitment
- 54
Group-I
1. Participants in the age group of 3 to 5 years with a diagnosis of infections and advised five days or more of oral or intravenous antibiotics
2. Parents willing to provide written informed consent
Group-II
1. Participants In the age group of 3 to 5 years attending clinics for vaccination, non-infectious diseases.
2. Parents willing to provide written informed consent
Group-I
1. Chronic illnesses including metabolic, neurological, allergic disorders
2. History of any other chronic illness, NICU & PICU admission
3. History of any acute childhood illness in the past week
4. History of drug intake in the last 3 months including antibiotics, PPIs, Probiotics
Group-II
1. Chronic illnesses including metabolic, neurological, allergic disorders
2. History of any other chronic illness, NICU & PICU admission
3. History of any acute childhood illness in the past week
4. History of drug intake in the last 3 months including antibiotics, PPIs, Probiotics
Study & Design
- Study Type
- Observational
- Study Design
- Not specified
- Primary Outcome Measures
Name Time Method To measure the quantitative and qualitative differences in relative abundance of gut microbiota composition between Group I and Group II including: <br/ ><br>? Phyla Firmicutes and Bacteroidetes, Firmicutes/Bacteroidetes Ratio <br/ ><br>? Genera Lactobacillus, Bifidobacterium, Bacteroides, Prevotella, Enterobacter, Roseburia, Clostridium and Escherichia <br/ ><br>? Microbial diversityTimepoint: Day 0 to Day 5
- Secondary Outcome Measures
Name Time Method To measure the biologic function of genera and species by analyzing stool samples using amplicon based 16s rRNA sequencing methodTimepoint: Day 0 to Day 5