Smoking and Peptidoglycan Recognition Protein-1 and Initial Periodontal Therapy

Not Applicable

Completed

• Conditions: Smoking, Cigarette
• Interventions: Other: Initial periodontal therapy

• Registration Number: NCT03915730
• Lead Sponsor: Kırıkkale University

Brief Summary

A total of 40 patients with CP (20 smokers (S+CP) and 20 nonsmokers (S-CP)) and 40 periodontally healthy subjects (20 smokers (S+PH) and 20 nonsmokers (S-PH)), comprising 80 subjects, were included in this study. Baseline GCF samples were obtained from all subjects and clinical periodontal measurements, including probing depth (PD), clinical attachment level (CAL), plaque index (PI), and gingival index (GI), were recorded. In patients who had received initial periodontal therapy, GCF samples were obtained and all clinical periodontal measurements were recorded again during the 6th-8th weeks. GCF PGRP-1 levels were analyzed by enzyme-linked immunosorbent assay.

Detailed Description

Peptidoglycan recognition proteins (PGRPs) are one of the antimicrobial peptides that play a role in innate immunity and act against the bacterial cell wall. Smoking is the most important environmental risk factor for periodontitis. This study determined the effect of smoking on PGRP-1 levels in gingival crevicular fluid (GCF) in smokers and nonsmokers with chronic periodontitis (CP) following initial periodontal therapy.

Study Timeline

• Study Start: 2016-08
• Primary Completion: 2017-07
• Study Completion: 2017-11
• Status Verified: 2019-04
• Study First Submitted: 2019-04-11
• Study First Submitted QC: 2019-04-14
• Last Update Submitted: 2019-04-14
• Study First Posted: 2019-04-16
• Last Update Posted: 2019-04-16

Recruitment & Eligibility

• Status: COMPLETED
• Sex: Not specified
• Target Recruitment: 80

Inclusion Criteria

• Clinical diagnosis of severe chronic periodontitis
• Clinical diagnosis of periodontal health

Exclusion Criteria

• have any systemic disease,
• to be alcohol consumer,
• to receive any periodontal therapy in the past 6 months,
• to take any drugs regularly
• to be pregnant and/or lactate.

Study & Design

• Study Type: INTERVENTIONAL
• Study Design: PARALLEL

Arm && Interventions

Group	Intervention	Description
Non-smokers with chronic periodontitis	Initial periodontal therapy	Patients who had teeth with 30% periodontal bone loss, presence of at least two nonadjacent sites per quadrant with probing pocket depths of ≥5 mm, and bleeding on probing were assigned as chronic periodontitis group Never smoked patients were included into the nonsmoker group
Smokers with chronic periodontitis	Initial periodontal therapy	Patients who had teeth with 30% periodontal bone loss, presence of at least two nonadjacent sites per quadrant with probing pocket depths of ≥5 mm, and bleeding on probing were assigned as chronic periodontitis group Patients who have smoked for at least 10 years and a minimum of 10 cigarettes per day were assigned as current smokers.

Primary Outcome Measures

Name	Time	Method
Change in peptidoglycan recognition protein-1	Baseline and 3 months	Total amount of Peptidoglycan Recognition Protein-1 (µg) in gingival crevicular fluid

Secondary Outcome Measures

Name	Time	Method
Change in periodontal clinical parameter	Baseline and 3 months	Clinical attachment level (mm) (from cemento-enamel junction to the base of periodontal pocket)