Effect of Different Centrifuge Values on Saliva High Sensitive C-Reactive Protein (Hs-CRP) Levels in Individuals With Different Smoking Amounts

Not Applicable

Recruiting

• Conditions: Smoking
• Interventions: Diagnostic Test: saliva obtaining

• Registration Number: NCT06561971
• Lead Sponsor: Izmir Katip Celebi University

Brief Summary

The purpose of this study; In periodontally healthy individuals, high sensitive c-reactive protein (hs-CRP) levels of non-smokers, smokers of less than 10 cigarettes per day and smokers of more than 10 cigarettes per day were measured at 15 rpm-10 min, 10 centrifuge values, which are frequently used in saliva studies. To determine the levels in saliva samples as a result of rpm-10 min and 10 rpm 6 min centrifugations and to determine the values at which a cytokine of this molecular weight should be centrifuged and to examine the possible correlation between these values and the clinical parameters of the amount of smoking. Materials and Methods: saliva samples were collected from 90 systemically healthy individuals who were non-smokers (N, n=30), light smokers \<10 cigarettes per day (L, n=30) and heavy smokers \>10 cigarettes per day (H, n=20). samples were taken. Full-mouth clinical periodontal measurements, including probing depth (PD), clinical attachment level (CAL), bleeding on probing (BOP), gingival index (GI), and plaque index (PI), were also recorded. Enzyme-linked immunosorbent assay (ELISA) was used to determine hs-CRP levels in biological samples.

Detailed Description

Not available

Study Timeline

• Study Start: 2024-06-01
• Primary Completion: 2024-08-01
• Study First Submitted: 2024-08-16
• Study First Submitted QC: 2024-08-19
• Study First Posted: 2024-08-20
• Status Verified: 2024-12
• Last Update Submitted: 2024-12-19
• Last Update Posted: 2024-12-20
• Study Completion: 2025-01-22

Recruitment & Eligibility

• Status: RECRUITING
• Sex: All
• Target Recruitment: 90

Inclusion Criteria

• Systemically healthy

• Periodontally clinical healthy

• At least 20 permanent teeth in the mouth

• No medication for continuous use

• Those who have not used antibiotics, anti-inflammatory and systemic corticosteroid drugs in the last 6 months.

• Not in pregnancy or lactation period.

• Not received periodontal treatment in the last 6 months

  • For the non-smoker group; person who does not smoke tobacco
  • For the light-smoker group; person who smokes less than 10 cigarettes a day
  • For heavy-smoker group; person who smokes more than 10 cigarettes a day

Exclusion Criteria

• Any oral or systemic disease
• Regularly using a systemic medication
• During pregnancy or lactation
• Received periodontal treatment within the last 6 months.
• Those who received antibiotic, anti-inflammatory or systemic corticosteroid medication in the last 6 months
• Individual with periodontal disease

Study & Design

• Study Type: INTERVENTIONAL
• Study Design: PARALLEL

Arm && Interventions

Group	Intervention	Description
Heavy Smokers	saliva obtaining	Full-mouth clinical periodontal measurements recorded and saliva obtained.
Light Smokers	saliva obtaining	Full-mouth clinical periodontal measurements recorded and saliva obtained.
Non-Smokers	saliva obtaining	Full-mouth clinical periodontal measurements recorded and saliva obtained.

Primary Outcome Measures

Name	Time	Method
The total amount of hs-CRP in saliva	24 hours after taking the clinical measurements at the first visit

Secondary Outcome Measures

Name	Time	Method
Determining the ideal centrifugal value range of hs-CRP saliva level	Within 1 week after receiving the biochemical analysis results

Trial Locations

Locations (1)

Izmir Katip Çelebi University Department of Periodontology; 🇹🇷 Izmir, Çiğli, Turkey