The Role of Sleep Deprivation in the Regulation of Immune System, Neuroendocrine Responses, and Behavioral Measures.
Overview
- Phase
- Not Applicable
- Intervention
- Not specified
- Conditions
- Sleep Deprivation
- Sponsor
- Uppsala University
- Enrollment
- 18
- Locations
- 1
- Primary Endpoint
- Neutrophil phagocytotic function production
- Last Updated
- 13 years ago
Overview
Brief Summary
The study proposes to investigate whether sleep deprivation will affect a variety of measures, including hormones, immune system functioning, and behaviors related to food intake and hunger. It is predicted that sleep deprivation will affect circulating neutrophil activity, and do so via affects on DNA methylation. It is also predicted that sleep deprivation will up-regulate ghrelin, and down-regulate circulating oxytocin. Finally, it is predicted that sleep deprivation will increase participants' tendencies to pick larger portions of food, and also increase their tendency to purchase foods that are more caloric in a mock supermarket scenario.
Investigators
Eligibility Criteria
Inclusion Criteria
- •Age 18-35y
- •Healthy (self-reported) and not on medication
- •Non-smoking
- •Normal sleep-wake rhythm (i.e. 7-8 h per night, self-reported)
Exclusion Criteria
- •Major illness
- •Taking any serious medications
- •Any sleep conditions (e.g. irregular bedtimes, sleep complaints)
- •Any dietary issues with the food items provided
- •A history of endocrine or psychiatric disorders
Outcomes
Primary Outcomes
Neutrophil phagocytotic function production
Time Frame: Change in 4 neutrophil phagocytotic function from baseline to 36hours later (after the nighttime intervention)
This project is designed to test the inflammatory capacity of circulating neutrophils. It also aims to investigate if any changes in neutrophil efficacy are governed by alterations in DNA methylation. Measured during 36 hours (at 1930 day 1, 0730 and 1930 day 2, 0730 day 3)
Secondary Outcomes
- Portion Size Task(Change in selected portion size from baseline to 1 hr after consuming a caloric preload)
- Neuro-economics task(Change in purchasing behaviour 1 hr after consuming a caloric preload)
- Circulating hormone levels(Change in circulating hormone levels from baseline (ie. 1930 - before sleep intervention) to 12hr later (0730 after the nighttime intervention))