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The Effects of Cold Adaptation on Skin Blood Flow, Hand Function, and Comfort in Healthy Adults

Not Applicable
Completed
Conditions
Cold Exposure
Interventions
Other: Repeated cold exposure
Registration Number
NCT05464758
Lead Sponsor
United States Army Research Institute of Environmental Medicine
Brief Summary

In cold weather environments, blood flow to the extremities is significantly reduced, which severely impairs hand function and induces thermal discomfort. Prolonged or repeated cold exposure elicits an adaptive habituation response that is characterized by blunted skin vasoconstriction and thus may be an effective strategy to improve peripheral perfusion, reduce thermal discomfort, and maintain hand function during cold weather military operations. Since mission conditions often involve low ambient temperatures, countermeasures that reduce cold-induced decrements in hand function and thermal comfort are important to enhance Warfighter readiness in cold weather battlefield environments. The goals of this study are to 1) evaluate the effectiveness of cold habituation in improving skin blood flow, hand function, and thermal comfort during cold exposure and 2) identify the mechanisms that contribute to improvements in skin blood flow following habituation.

Detailed Description

Participants (n=15, 18-39 yrs) will complete a preliminary cold air exposure (baseline testing) followed by 8 days of repeated cold air exposures with minimal clothing. The cold exposures will be 2 hours in duration and will be conducted in 8°C (46°F) conditions. Before and after the repeated cold exposures, participants will undergo hand function tests, assessments of thermal comfort, and skin blood flow testing.

Recruitment & Eligibility

Status
COMPLETED
Sex
All
Target Recruitment
16
Inclusion Criteria
  • Male or female, age 18-39
  • In good health as determined by OMSO (Office of Medical Support and Oversight)
  • Willing to refrain from exercise and caffeinated or alcoholic beverages for 12 hours before each testing session
  • Willing to refrain from smoking or tobacco use 8 hours before each testing session
Exclusion Criteria
  • History of cold injuries of any severity (e.g., frostbite, trench foot, chilblains).
  • Raynaud's syndrome.
  • Cold-induced asthma/bronchospasm
  • Previous hand/finger injuries that impair dexterity and hand function.
  • Metal hardware (plates/screws) in the forearms and hands.
  • Current use of medications or dietary supplements that could alter cardiovascular, thermoregulatory, or vascular control (e.g., anti-hypertensives, statins).
  • Known allergies to medical adhesives.
  • Heart, lung, kidney, muscle, or nerve disorder(s)
  • A planned MRI during the study or within 2 days after completing a cold test.
  • Women who are pregnant, planning to become pregnant, or breastfeeding.
  • Not willing to have small areas of skin on the body shaved (if deemed necessary for attachment of study instrumentation)

Study & Design

Study Type
INTERVENTIONAL
Study Design
SINGLE_GROUP
Arm && Interventions
GroupInterventionDescription
Cold habituationRepeated cold exposure8-day cold habituation
Primary Outcome Measures
NameTimeMethod
Skin blood flow8 days

Skin blood flow will be measured using non-invasive laser-Doppler flowmetry probes before and during cold exposure. Output (red blood cell flux) will be divided by mean arterial blood pressure to give an index of cutaneous vascular conductance \[CVC, flux/mmHg\].

Skin temperature8 days

Skin temperatures \[in °C\] at up to 10 sites on the body will be measured using skin thermistors before and during cold exposure. Hand and foot skin temperatures will also be measured with a thermography imaging camera.

Manual dexterity - Pegboard3 days

Pegboard scores will be obtained before and during cold exposure. Scores are the number of pegs placed into the boards.

Manual dexterity - Minnesota Rate of Manipulation3 days

Minnesota Rate of Manipulation dexterity scores will be obtained before and during cold exposure. Scores are the time \[in seconds\] required to move small blocks various distances.

Hand and finger strength3 days

Pinch and hand strength \[in kg\] will be measured using a pinch meter and grip strength device (dynamometer). Participants will complete three pinch strength tests and one grip strength test before and during cold exposure.

Cold perception5 days

Ratings of thermal comfort, thermal sensation, and pain perception \[in arbitrary units\] will be assessed using visual analogue scales before and during cold exposure.

Secondary Outcome Measures
NameTimeMethod
Metabolic heat production5 days

Oxygen consumption and carbon dioxide production \[ml/kg/min\] will be measured using computerized indirect calorimetry before and during cold exposure. Oxygen consumption and carbon dioxide production will be combined to report metabolic heat production in Watts per meter squared.

Plasma norepinephrine3 days

Circulating concentrations of norepinephrine \[in pg/ml\] will be assessed in blood collected via venipuncture before and after cold exposure.

Heart rate12 days

Heart rate \[in beats per min\] will be measured using an automated cuff before and during cold exposure.

Body core temperature5 days

Body core temperature \[in °C\] will be measured using a telemetry pill self-inserted as a suppository during cold exposure.

Blood pressure12 days

Brachial artery blood pressure \[in mmHg\] will be measured using an automated blood pressure cuff before and during cold exposure.

Trial Locations

Locations (1)

U.S. Army Research Institute of Environmental Medicine

🇺🇸

Natick, Massachusetts, United States

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