Sedentary Behavior, Cardiovascular Function, and Sleep
Overview
- Phase
- N/A
- Intervention
- Not specified
- Conditions
- Sedentary Behavior
- Sponsor
- Oregon Health and Science University
- Enrollment
- 20
- Locations
- 1
- Primary Endpoint
- Endothelial Function
- Last Updated
- 3 years ago
Overview
Brief Summary
This is an interventional study that will examine how sedentary behavior (decreased physical inactivity) over time affects cardiovascular health (i.e. heart rate and blood pressure) and sleep quality/duration.
Detailed Description
PRIMARY OBJECTIVES: 1. To test the hypothesis that two weeks of sedentary behavior will progressively impair vascular endothelial function. 2: To test the hypothesis that increasing sedentary behavior will decrease total sleep duration and sleep efficiency. 3: To test the hypothesis that two-weeks of sedentary behavior will progressively increase 24-h blood pressure and reduce nocturnal blood pressure dipping. OUTLINE: After a baseline period, participants will become more inactive and also come in for 4-h uninterrupted sitting visits. Cardiovascular, activity, and sleep measures will be taken throughout.
Investigators
Saurabh Thosar
Principal Investigator
Oregon Health and Science University
Eligibility Criteria
Inclusion Criteria
- •Ages 20-80y
- •Lean and overweight (BMI 18.5-40 kg/m2)
- •No acute, chronic, or debilitating medical conditions
- •No prescription/non-prescription medications or drugs of abuse
- •Limited weight training or intense exercise (swimming, CrossFit)
- •Non-smoker
- •Average level of daily physical activity (8,000-12,499 steps/day)
- •Persons who fit all the above criteria and are suitable based on a medical history and health habits questionnaire, sleep profiling questionnaire, and electrocardiogram and clinical biochemical screening tests of blood and urine may be eligible to participate.
Exclusion Criteria
- •Persons with any acute, chronic, or debilitating medical condition except pre-hypertension and/or mild to moderate sleep apnea will be excluded.
- •Persons with any symptoms of acute or active illness (e.g. fever, leukocytosis) will be excluded.
- •Persons with a history of severe psychiatric illness or psychiatric disorders will be excluded.
- •Persons with a history of regular night/or rotating shift work, or who have traveled more than three time zones during the one month prior to the study will be excluded.
- •Pregnant persons, decisionally impaired adults, and prisoners will be excluded.
Outcomes
Primary Outcomes
Endothelial Function
Time Frame: 3 weeks
Investigators will measure endothelial function as flow-mediated dilation at baseline, and each hour of the 3-hour sitting trials.
Oxidative stress
Time Frame: 3 weeks
Oxidative stress will be measured using malondialdehyde adducts (MDA) from Ethylenediaminetetraacetic acid (EDTA) plasma.
Endothelin-1
Time Frame: 3 weeks
Endothelin 1 (ET-1) will be measured from Ethylenediaminetetraacetic acid (EDTA) plasma.
Heart rate
Time Frame: 3 weeks
During the weekly trials, heart rate will be measured as beats per minute while participants are in a seated position during the prolonged sitting period.
Blood pressure
Time Frame: 3 weeks
During the weekly trials, blood pressure will be measured as systolic over diastolic in a seated position in the dominant arm
24-hour BP measurement
Time Frame: 3 weeks
Investigators will measure 24-hour ambulatory blood pressure monitoring (AMBP, Spacelabs, Inc) at baseline and for 24-h before each in-lab visit.
Sleep duration
Time Frame: 3 weeks
Sleep duration will be scored from the ActiGraph and correlated with the sleep and activity diary.
Sleep efficiency
Time Frame: 3 weeks
Sleep efficiency will be scored from the ActiGraph and correlated with the sleep and activity diary.
Activity
Time Frame: 3 weeks
Activity will be scored from the ActiGraph and correlated with the sleep and activity diary.
Activity Perception
Time Frame: 3 weeks
Activity perception will be measured before each prolonged sitting trial using an Activity Perception questionnaire (Perception of Current Activity Questionnaire). The questionnaire has 7 questions with answers ranging from 1 to 5. The range of scores is from 7 to 35, with 7 suggesting a strong negative perception of current activity, and 35 suggesting a strong positive perception of current activity.