Metabolic Profiling of Peripheral Blood Mononuclear Cells (PBMCs) by LC-MS in Prostate Cancer Patients
试验速览
- 阶段
- 不适用
- 状态
- 终止
- 入组人数
- 10
- 试验地点
- 1
- 主要终点
- Blood sample collection
研究概览
简要总结
This pilot research trial utilizes the collection of blood in studying metabolites in patients with prostate cancer. Metabolites are the small molecule products of cellular metabolism that are produced naturally in all living cells. Collecting blood in order to study metabolites may help doctors monitor and treat prostate cancer more effectively.
详细描述
PRIMARY OBJECTIVES:
I. Develop blood sample collection and preparation procedures for reliable, meaningful metabolomic profiling of peripheral blood mononuclear cells (PBMCs) by liquid chromatography/quadruple-time of flight/mass spectrometry (LC/Q-TOF/MS) that can be implemented in a clinical setting.
II. Optimize data analysis methods and software usage to create a metabolic profile for patients at the time of blood collection.
III. Compare metabolite profiles of isolated PBMCs versus (vs.) other blood fractions (plasma, red blood cells [RBCs], and whole blood).
IV. Identify elements of the PBMC metabolite profile that may correlate to patient disease state.
研究设计
- 研究类型
- Observational
- 观察模型
- Case Only
- 时间视角
- Prospective
入排标准
- 年龄范围
- 18 Years 至 —（Adult, Older Adult）
- 性别
- Male
- 接受健康志愿者
- 否
入选标准
- •All subjects will have histologically confirmed prostate cancer
- •Provide written consent/authorization to participate in this study
- •Have no signs or symptoms of active infection
- •Exhibit a willingness to comply with the experimental procedures as outlined in this protocol
排除标准
- 未提供
结局指标
主要结局
Blood sample collection
时间窗: Baseline
The blood will be used to analyze the metabolites produced by white blood cells.
次要结局
未报告次要终点