Serum Sclerostin Level in Patients With Vitamin D Deficiency
试验速览
- 阶段
- 不适用
- 状态
- 已完成
- 发起方
- 入组人数
- 26
- 试验地点
- 1
- 主要终点
- Serum sclerostin level
研究概览
简要总结
The aim of this study is to determine serum sclerostin levels and change in serum sclerostin levels in patients with Vitamin D deficiency treated with calcium and vitamin D.
Healthy premenopausal Patients with Vitamin D deficiency diagnosed and routinely treated with calcium and vitamin D will be included in the study. This is an observational study. The serum sclerostin levels will be measured before and after Vitamin D treatment.
详细描述
This study is a prospective, observational, single-center study. Ethical approval was obtained from the Institutional Review Board.
This study will be completed with patients with Vitamin D deficiency. An intravenous cannula will be inserted into the antecubital vein. Blood samples will be obtained before and after treatment.
Serum will be collected and will be centrifuged for 15 minutes at 1000Xg within 30 minutes of collection. Aliquots of serum will be added to eppendorf tubes and stored at -20°C.
Serum sclerostin levels will be measured using a Human Sclerostin ELISA kit (Cusabio, Catalog No: CSB-E13146h, Newark, DE, USA). All assays will be performed according to the manufacturer's instructions. The minimum detectable concentration of human sclerostin is typically <0.012 ng/ml. Intra-assay precision is less than 8%.
研究设计
- 研究类型
- Observational
- 观察模型
- Case Only
- 时间视角
- Prospective
入排标准
- 年龄范围
- 20 Years 至 50 Years（Adult）
- 性别
- Female
- 接受健康志愿者
- 否
入选标准
- •Premenopausal
- •Serum 25(OH)D < 10 ng/mL
排除标准
- •Tumor-induced osteomalacia (hypophosphatemia)
- •Hypothyroidism
- •Hepatic / renal disease
- •Primary hyperparathyroidism
- •Fibromyalgia
- •Polymyalgia rheumatica
- •Serum 25(OH)D > 10 ng/mL
- •Vitamin D and calcium treatment commenced prior to study participation
结局指标
主要结局
Serum sclerostin level
时间窗: 8 weeks
Changes in serum sclerostin level in patients with osteomalacia who are scheduled for routine treatment of osteomalacia
次要结局
未报告次要终点
研究者
Ilhan Karacan, Clinical Associated Professor
Chief of Physical Med & Rehab Clinic
Bagcilar Training and Research Hospital