A pilot study about culturing laryngeal tumor tissue organoids to facilitate new treatment strategies for laryngeal cancer in the future
Recruiting
- Conditions
- Laryngeal cancerLaryngeal carcinoma10027656
- Registration Number
- NL-OMON55128
- Lead Sponsor
- niversitair Medisch Centrum Groningen
- Brief Summary
Not available
- Detailed Description
Not available
Recruitment & Eligibility
- Status
- Recruiting
- Sex
- Not specified
- Target Recruitment
- 20
Inclusion Criteria
- proven carcinoma of the larynx.
- > 18 yrs of age
- planned routine biopsy or planned surgical resection as part of standard
diagnostic work-up or treatment
- expected tumor volume > 2 cm3
- informed consent
Exclusion Criteria
- no squamous cell carcinoma after definitive histolopathological analysis
Study & Design
- Study Type
- Observational non invasive
- Study Design
- Not specified
- Primary Outcome Measures
Name Time Method <p>In ex vivo LC organoids: viable and proliferating organoids after at least 2<br /><br>and 6 weeks in 50% of the samples.<br /><br>To assess viability and to confirm the origin of each tumor specimen, the<br /><br>specimen will be evaluated by analysis of non-necrotic tumor tissue in<br /><br>hematoxylin-eosin stained tissue sections and by determining proliferation rate<br /><br>on day 0, week 2 and week 6, using organoid formation and growth rate and by<br /><br>using whole genome DNA sequencing (~30x coverage (~100 Gb)). </p><br>
- Secondary Outcome Measures
Name Time Method <p>We will analyse differences in solidity of ex vivo LC organoids between primary<br /><br>and recurrent LC tissue by using a Chi-square test with SPSS. </p><br>
Related Research Topics
Explore scientific publications, clinical data analysis, treatment approaches, and expert-compiled information related to the mechanisms and outcomes of this trial. Click any topic for comprehensive research insights.
What molecular mechanisms do laryngeal tumor organoids reveal for developing targeted therapies in laryngeal carcinoma?
How effective are organoid-based models compared to traditional 2D cultures in predicting laryngeal cancer treatment responses?
Which biomarkers are associated with laryngeal tumor organoid growth and could predict patient outcomes in laryngeal carcinoma?
What are the potential adverse events linked to organoid culture techniques in laryngeal cancer research and how are they managed?
Are there any combination therapies being explored with laryngeal tumor organoids to enhance treatment efficacy for laryngeal carcinoma?