Identification of the molecular basis of anti-cancer effect of Huaier.
Not Applicable
Recruiting
- Conditions
- Cancers such as hepatocellular carcinoma, breast cancer, colorectal cancer and related gastrointestinal cancers, urologic cancers including prostate cancer, pancreas cancer, and lung cancer), on which a significant therapeutic efficacy of Huaier has been reported. Special remarks is emphasized on the cancer cases designated to be related to cancer stem cells.
- Registration Number
- JPRN-jRCT1091220335
- Lead Sponsor
- Manami Tanaka, M.D., Ph.D., Bradeion Institute of Medical Sciences, Co. Ltd.
- Brief Summary
Not available
- Detailed Description
Not available
Recruitment & Eligibility
- Status
- RECRUITING
- Sex
- All
- Target Recruitment
- 80
Inclusion Criteria
Diagnosed as cancer and will be obtained follow-up data and healthy persons
Exclusion Criteria
Taking other medicines or supplements.
Study & Design
- Study Type
- Interventional
- Study Design
- Not specified
- Primary Outcome Measures
Name Time Method For mRNA libraries, focus on mRNA studies. Data analysis includes sequencing data processing and basic sequencing data quality control, prediction of new transcripts, differential expression analysis of genes. Gene Ontology (GO) and the KEGG pathway database are used for annotation and enrichment analysis of up-regulated genes and down-regulated genes.<br>For small RNA libraries, data analysis includes sequencing data process and sequencing data process QC, small RNA distribution across the genome, rRNA, tRNA, alignment with snRNA and snoRNA, construction of known miRNA expression pattern, prediction New miRNA and Study of their secondary structure Based on the expression pattern of miRNA, we perform not only GO / KEGG annotation and enrichment, but also different expression analysis.
- Secondary Outcome Measures
Name Time Method Based on subtle expression results and small RNA and mRNA prediction results, we perform integrated analysis to identify related small RNA and mRNA.