Chronic Kidney Disease in Relation to Alterations in Protein and Amino Acid Metabolism and Function
试验速览
- 阶段
- 不适用
- 状态
- 撤回
- 试验地点
- 2
- 主要终点
- Net whole-body protein synthesis
研究概览
简要总结
Weight loss commonly occurs in patients with chronic kidney disease (CKD), negatively influencing their quality of life, treatment response and survival. Loss of muscle protein is generally a central component of weight loss in CKD patients but patients also have reductions in fat mass and bone density, independent of the severity of the disease state. Attempts to reverse weight and muscle loss in CKD and improve nutritional status by nutritional supplementation have been unsuccessful and there are currently no approved therapies.
Purpose of this study is to provide detailed insight in disease related gut function by obtaining information on gut permeability, digestion and absorption of glucose, fat and protein in CKD patients compared to matched healthy controls. Additionally, to examine whether protein and amino acid metabolism is disturbed in CKD patients compared to healthy controls. This will provide required information that will lead to implement new strategies to develop optimal nutritional regimen in order to enhance nutritional status, quality of life and survival in relation to kidney disease.
详细描述
This study involves one test day of approximately 7-8 hours. On this test day subjects will ingest a sugar drink to assess gut permeability and gut function, and a protein meal to measure digestion/absorption and the anabolic response to food intake. Subjects will also receive a mixture of amino acids that are made a little heavier than normal, called stable isotopes. This stable isotopes is used to investigate protein behavior in the body (protein kinetics). Blood (100-120 ml in total) and urine samples will be collected over 7.5 hours.
研究设计
- 研究类型
- Interventional
- 分配方式
- Na
- 干预模型
- Single Group
- 主要目的
- Supportive Care
- 盲法
- None
入排标准
- 年龄范围
- 55 Years 至 —（Adult, Older Adult）
- 性别
- All
- 接受健康志愿者
- 是
入选标准
- 未提供
排除标准
- 未提供
结局指标
主要结局
Net whole-body protein synthesis
时间窗: 0, 15, 30, 45, 60, 75, 90, 105, 120, 150, 180, 210 min post-meal
Change in whole-body protein synthesis rate after intake of meal
次要结局
- Cognitive function(1 day)
- Tryptophan turnover rate(Postabsorptive state during 3 hours)
- Myofibrillar protein breakdown rate(0,15,30,45,60,75,90,105,120,150,180,210 min post-meal)
- Glycine rate of appearance(Postabsorptive state during 3 hours)
- Skeletal and respiratory muscle strength(1 day)
- Glucose absorption(7 hours)
- Gut permeability(7 hours)
- Insulin response to feeding(During 3 hours after feeding)
- Citrulline Rate of appearance(Postabsorptive state during 3 hours)
- Fatty acid digestion after feeding(0,15,30,45,60,75,90,105,120,150,180,210 min post-meal)
- Protein digestion after feeding(0,15,30,45,60,75,90,105,120,150,180,210, min post-meal)
- Arginine turnover rate(Postabsorptive state during 3 hours)
- Whole body collagen breakdown rate(Postabsorptive state during 3 hours)
- Fat-free mass(Postabsorptive state during 15 min)
- Taurine turnover rate(Postabsorptive state during 3 hours)
研究者
Marielle PKJ Engelen, PhD
PhD
Texas A&M University