Lipid-induced Insulin Resistance is Not Mediated by Impaired Transcapillary Transport of Insulin and Glucose in Humans
试验速览
- 阶段
- 不适用
- 状态
- 已完成
- 发起方
- 入组人数
- 8
- 试验地点
- 2
- 主要终点
- Blood flow
研究概览
简要总结
There is a current debate whether impaired insulin-mediated microvascular perfusion limits the delivery of hormones and nutrients to muscle and whether short term FFA elevation affects transcapillary transport of insulin and glucose thereby representing a rate-controlling step for insulin-stimulated muscular glucose disposal in humans.
To address these questions, the investigators determined the changes of interstitial glucose and insulin in skeletal muscle of healthy volunteers during intravenous administration of triglycerides or glycerol under physiologic and supraphysiologic hyperinsulinemic conditions.
详细描述
Increased lipid availability reduces insulin-stimulated glucose disposal in skeletal muscle, which is generally explained by lipid induced inhibition of myocellular insulin signalling, It remains unclear whether lipids also impair transcapillary transport of insulin and glucose which could thereby become rate-controlling for glucose disposal Increased accumulation and availability of lipids cause impaired skeletal muscle insulin sensitivity. It is yet unclear if transcapillary transport of insulin and glucose is impaired by acute elevation of free fatty acids and represents a rate-limiting step during the development of short-term lipid-induced insulin resistance. We determined the changes of interstitial glucose and insulin in skeletal muscle of healthy volunteers during intravenous administration of triglycerides and heparin or glycerol under physiologic and supraphysiologic hyperinsulinemic conditions.
研究设计
- 研究类型
- Interventional
- 分配方式
- Randomized
- 干预模型
- Crossover
- 主要目的
- Basic Science
- 盲法
- Single (Participant)
入排标准
- 年龄范围
- 20 Years 至 45 Years（Adult）
- 性别
- Male
- 接受健康志愿者
- 是
入选标准
- •The volunteers have to be older than 19 years,
- •nonobese (body mass index, BMI less than 27 kg/m2),
- •normolipidemic (fasting serum concentration of triglycerides < 140 mg/dL and
- •total cholesterol < 200 mg/dL) and
- •non-smokers.
排除标准
- •The following exclusion criteria will be applied:
- •any medication within two weeks prior to the start of study,
- •regular alcohol consumption > 40 g/d,
- •acute inflammatory disease defined by serum C-reactive protein > 1 mg/dL,
- •abnormalities in the screening visit or in laboratory tests considered as clinically relevant,
- •family history of diabetes mellitus or dyslipidemia,
- •glucose intolerance,
- •allergy or hypersensitivity against study medication,
- •blood clotting disorders.
结局指标
主要结局
Blood flow
时间窗: between the start of the lipid/glycerol infusion until the end of the study (360 min)
Regional blood flow. Muscular blood flow will be measured by the laser Doppler flow technique (LDF, Moor Instruments, Devon, UK) as described previously
次要结局
- Interstitial insulin concentration(between the start of the lipid/glycerol infusion until the end of the study (360 min))