Evaluation of Gas6 and Soluble MerTK Levels in Gingival Crevice Fluid in Individuals With Periodontal Health, Gingivitis, and Periodontitis
Trial Snapshot
- Phase
- Not Applicable
- Status
- Recruiting
- Enrollment
- 90
- Locations
- 1
- Primary Endpoint
- Gas6 Levels in Gingival Crevicular Fluid
Study Overview
Brief Summary
This study aimed to investigate gingival crevicular fluid (GCF) and serum soluble MerTK (sMerTK) and Gas6 levels in periodontal health and disease. A total of 90 individuals, 30 patients with stage III grade B periodontitis, 30 with gingivitis and 30 periodontally healthy individuals were included. Whole-mouth and site-specific clinical periodontal parameters including probing depth, clinical attachment level, bleeding on probing, gingival index, plaque index were recorded. GCF and serum sMerTK and Gas6 levels were measured by enzyme-linked immunosorbent assay. Statistical analysis was performed by using non-parametric tests.
Study Design
- Study Type
- Observational
- Observational Model
- Other
- Time Perspective
- Cross Sectional
Eligibility Criteria
- Ages
- 18 Years to — (Adult, Older Adult)
- Sex
- All
- Accepts Healthy Volunteers
- Yes
Inclusion Criteria
- •Individuals without systemic diseases that may affect periodontal tissues (such as cardiovascular diseases, atherosclerosis, immune system disorders, rheumatoid arthritis, obesity, cancer, and diabetes)
- •Individuals who have not used antibiotics within the last 3 months
- •Individuals not taking any medications known to affect periodontal tissues
- •Individuals without infectious diseases such as HIV, hepatitis B virus (HBV), or tuberculosis
- •Individuals with at least 16 natural teeth, excluding third molars
- •Individuals aged 18 years and older
Exclusion Criteria
- •Pregnant or lactating individuals
- •Individuals who have received periodontal treatment within the last 6 months
- •Current smokers
Arms & Interventions
periodontally healthy (H) group
Intervention: gingival crevicular fluid collection (Other)
gingivitis (G) group
Intervention: gingival crevicular fluid collection (Other)
stage III grade B periodontitis (P) group
Intervention: gingival crevicular fluid collection (Other)
Outcomes
Primary Outcomes
Gas6 Levels in Gingival Crevicular Fluid
Time Frame: baseline
Quantitative measurement of Gas6 levels (pg/mL) in gingival crevicular fluid samples using enzyme-linked immunosorbent assay (ELISA), and comparison of these levels among periodontally healthy, gingivitis, and periodontitis groups.
Soluble MerTK (sMerTK) Levels in Gingival Crevicular Fluid
Time Frame: baseline
Quantitative measurement of soluble MerTK (sMerTK) levels (pg/mL) in gingival crevicular fluid samples using enzyme-linked immunosorbent assay (ELISA), and comparison of these levels among periodontally healthy, gingivitis, and periodontitis groups.
Secondary Outcomes
- Plaque Index(baseline)
- Probing Depth(baseline)
- Clinical Attachment Loss(baseline)
- Gingival Index(baseline)
Investigators
nuray Ercan
Assistant Professor
Abant Izzet Baysal University