Effect of Periodontal Treatment on Visfatin, fetuin-a and Sirtuin 1 of Patients With Periodontitis and Type 2 Diabetes
- Conditions
- PeriodontitisType 2 DiabetesPeriodontal Diseases
- Registration Number
- NCT05662475
- Lead Sponsor
- Ondokuz Mayıs University
- Brief Summary
The aim of this study is to determine the concentrations of Visfatin, Fetuin-A and Sirtuin 1 in the gingival crevicular fluid and clinical periodontal parameters in diabetic and systemically healthy individuals and to determine whether non-surgical periodontal treatment had any effect on these biomarkers and periodontal clinical parameters at the end of a 3-month follow-up period. The hypothesis of our study is that gingival crevicular fluid Visfatin, Fetuin-A and Sirtuin 1 concentrations will change with non-surgical periodontal treatment in type 2 diabetic and systemically healthy individuals and that this change will be associated with diabetes and clinical parameters.
- Detailed Description
Sixty-six patients were divided into six equal groups; group 1: systemically and periodontally healthy individuals, group 2: systemically healthy individuals with periodontitis, group 3: controlled type 2 diabetes and periodontally healthy individuals, group 4: controlled type 2 diabetes and periodontitis, group 5: uncontrolled type 2 diabetes and periodontally healthy individuals, group 6: uncontrolled type 2 diabetes and periodontitis. The study was planned as a randomized, single-blind, parallel design. Periodontal clinical parameters (Silness-Löe plaque index, Löe-Silness gingival index, probing pocket depth, clinical attachment level, bleeding on probing) were recorded clinically and concentrations of visfatin, fetuin-A and sirtuin 1 in gingival crevicular fluid were assessed biochemically. After baseline examinations, periodontal treatment was completed using a nonsurgical periodontal treatment protocol completed in 24 hours. Periodontal clinical parameters and gingival crevicular fluid were recorded at baseline and 3 months after periodontal treatment. Biochemical analysis and statistical evaluation were performed.
Recruitment & Eligibility
- Status
- COMPLETED
- Sex
- All
- Target Recruitment
- 66
- Volunteering to participate in the study
- To be over 18 years old
- No periodontal treatment in the last 6 months
- Being systemically healthy except for type 2 diabetes
- Not taking medication for any reason except type 2 diabetes
- HbA1c <7 in controlled Type 2 diabetes group
- HbA1c ≥7 in uncontrolled Type 2 diabetes group
- Not smoking or drinking alcohol
- Not volunteering to participate in the study
- Under 18 years of age
- Periodontal treatment in the last 6 months
- Having any systemic disease affecting the periodontal condition
- Having used local or systemic antibiotics in the last 3 months
- Use of anti-inflammatory, steroid drugs in the last 3 months
- Taking vitamin, mineral or antioxidant supplements in the last 3 months
- Being pregnant or lactating
- Regular use of mouthwash
- Smoking or drinking alcohol
Study & Design
- Study Type
- INTERVENTIONAL
- Study Design
- PARALLEL
- Primary Outcome Measures
Name Time Method Bleeding on probing index (BPI) Baseline to 3 months after treatment BPI was increased in all periodontitis patients with or without type 2 diabetes. Type 2 diabetes has no effect on BPI in periodontitis patients. BPI was reduced after NSPT.
Visfatin concentration in GCF Baseline to 3 months after treatment Visfatin concentrations in GCF were increased in all periodontitis patients. In periodontal healthy patients with Type 2 diabetes visfatin concentrations in GCF were increased. Non-surgical periodontal treatment decreased the concentration of visfatin in GCF.
Gingival crevicular fluid (GCF) quantity Baseline to 3 months after treatment The amount of gingival crevicular fluid was not affected by periodontal status in patients with Type 2 diabetes.
Clinical attachment level (CAL) Baseline to 3 months after treatment CAL was increased in all periodontitis patients with or without type 2 diabetes. Type 2 diabetes has no effect on CAL in periodontitis patients. CAL was reduced after NSPT.
Plaque index (PI) Baseline to 3 months after treatment PI was increased in all periodontitis patients with or without type 2 diabetes. Type 2 diabetes has no effect on PI in periodontitis patients. PI was reduced after non-surgical periodontal treatment (NSPT).
Gingival index (GI) Baseline to 3 months after treatment GI was increased in all periodontitis patients with or without type 2 diabetes. Type 2 diabetes has no effect on GI in periodontitis patients. GI was reduced after NSPT.
HbA1c level in blood plasma Baseline to 3 months after treatment NSPT reduced HbA1c levels of patients with type 2 diabetes.
Sirtuin 1 concentration in GCF Baseline to 3 months after treatment In systemic healthy individuals, periodontitis decreased the concentration of sirtuin 1 in GCF. Type 2 diabetes increased sirtuin 1 concentration in GCF. In controlled type 2 diabetes patients with periodontitis sirtuin 1 concentrations in GCF were increased. In uncontrolled type 2 diabetes patients with periodontitis sirtuin 1 concentrations in GCF were decreased. Non-surgical periodontal treatment increased the concentration of sirtuin 1 in GCF.
Probable pocket depth (PPD) Baseline to 3 months after treatment PPD was increased in all periodontitis patients with or without type 2 diabetes. Type 2 diabetes has no effect on PPD in periodontitis patients. PPD was reduced after NSPT.
Fetuin-A concentration in GCF Baseline to 3 months after treatment Fetuin-A concentrations in GCF were decreased in all periodontitis patients. In periodontal healthy patients with Type 2 diabetes fetuin-A concentrations in GCF were increased. Non-surgical periodontal treatment increased the concentration of fetuin-A in GCF.
- Secondary Outcome Measures
Name Time Method
Trial Locations
- Locations (1)
Ondokuz Mayıs University, Faculty of Dentistry, Department of Periodontology
🇹🇷Samsun, Turkey
Ondokuz Mayıs University, Faculty of Dentistry, Department of Periodontology🇹🇷Samsun, Turkey