Quantitative investigation of the activation of the cellular immune system by taste producing substances and 2-phenylethylamine from coffee, cocoa, diet lemonade, ginger tea, cabbage, and soy sauce.
Not Applicable
Recruiting
- Conditions
- cellular immunsystem
- Registration Number
- DRKS00005083
- Lead Sponsor
- Deutsche Forschungsanstalt für Lebensmittelchemie Leibniz InstitutPhysiologie
- Brief Summary
Not available
- Detailed Description
Not available
Recruitment & Eligibility
- Status
- Recruiting
- Sex
- All
- Target Recruitment
- 60
Inclusion Criteria
healthy
Exclusion Criteria
taking medication, smoking, BMI >25, competetive sports, known metabolic disorders e.g. malabsorption syndrome, lipid dysfunctions, diabetes mellitus, thyroid dysfunction
Study & Design
- Study Type
- interventional
- Study Design
- Not specified
- Primary Outcome Measures
Name Time Method activated leucocytes, genregulation of specific biomarkers (cytokines and their receptors) using ELISA, regulation of the expression of the particular chemosensory receptors (RT-qPCR).
- Secondary Outcome Measures
Name Time Method Quantitative analytics of saccharin, caffeine, goitrin, 2-phenylethylamine, glutamate / piperonyl-n-heptylamine, and gingerol will be performed using stable-isotope-dilution analysis and LC-MS/MS. Plasma (100 µl) will be spiked with the respective isotope labelled internal standard. After equilibration samples will be diluted with acetonitrile, the precipitate will be centrifuged and the supernatant will be reduced in N2. After reconsitutation with H2O the solution will be analyzed by HPLC-MS/MS.