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Prospective Study of Mylotarg and G-CSF in Acute Myeloid Leukemia Treatment

Phase 2
Completed
Conditions
Novo Acute Myeloid Leukemia
Interventions
Registration Number
NCT01698879
Lead Sponsor
Grupo Cooperativo de Estudio y Tratamiento de las Leucemias Agudas y Mielodisplasias
Brief Summary

Acute myeloid leukemia (AML) is a neoplasm of immature hematopoietic cells (blasts) with altered ripening capacity. Due to excessive proliferation, the blasts displace normal hematopoietic cells and bone marrow failure appears. Leukemic cells also infiltrate extramedullary tissues.

Following the standard chemotherapy treatment, the CR rate achieved is around 65-75% for all patients and 15% lower when considering only patients over 65 years. Modifications to the standard regimen consist of replacing the DNR for a cytotoxic one, modifying the dose of ara-C or adding a third drug.

Gemtuzumab ozogamicin (Mylotarg ®) is an immunoconjugate between anti-CD33 antibody and a cytotoxic antitumor antibiotic, calicheamicin. Mylotarg ® antibody specifically binds to CD33, a sialic acid-dependent adhesion protein expressed in over 90% of LMA10. Mylotarg ® selectively transports the cytotoxic agent calicheamicin into leukemic cells and hematopoietic progenitors differentiated from the myelomonocytic line, while respecting the pluripotent hematopoietic stem cells. Calicheamicin is released only after the fixation of the antibody anti-CD33 and its internalization by the cell, after which binds to and damages the DNA.

Mylotarg ® is approved in the U.S. for the treatment of CD33 positive AML in first relapse, for patients older than 60 years non-candidates for other intensive treatment modalities.

Since the efficacy of Mylotarg ® is equivalent and its toxicity profile less than the conventional therapy, it is logical to conduct a phase II trial exploring the role of Mylotarg ® in the early stages of treatment of AML.

Previous experience with gemtuzumab ozogamicin in relapsed patients led to its use combined with induction chemotherapy. The aim was to improve the CR rate reached with the latter and reduce relapse after achieving greater leukemic cytoreduction.

Recent data from the HOVON group support that the administration of G-CSF before and during induction chemotherapy decreases the incidence of relapse in patients with AML, particularly those considered to have intermediate risk.

Everything mentioned above justifies to investigate the combination of GO combined with chemotherapy with IDR and ara-C in standard 3x7 scheme and analyze the effect of sensitization with G-CSF in patients with AML de novo. If the treatment proposed here is effective and presents an acceptable toxicity it should be investigated.

Detailed Description

Not available

Recruitment & Eligibility

Status
COMPLETED
Sex
All
Target Recruitment
46
Inclusion Criteria
  1. Patients with primary or "de novo" AML, different than promyelocytic or M3 subtype.
  2. Age 18 to 70 years.
  3. Written informed consent form
Exclusion Criteria
  1. Acute leukemia appeared after a myeloproliferative process or a myelodysplastic syndrome longer than 6 months, AML arising after another cured malignant disease (e.g. Hodgkin's disease), and secondary AML treated with alkylating agents or radiation.
  2. Acute promyelocytic leukemia.
  3. Relevant history of liver disease. Significant impaired liver function (bilirubin, AST or ALT ≥ 2.5 times the normal value) not attributable to leukemic infiltration.
  4. Patients with prior heart failure.
  5. Symptomatic chronic respiratory failure.
  6. Positive serology for HIV, hepatitis C virus or its surface antigen.
  7. Estimated life expectancy less than 3 months, despite treatment.
  8. Pregnancy or breastfeeding at the time of inclusion in the study.

Study & Design

Study Type
INTERVENTIONAL
Study Design
SINGLE_GROUP
Arm && Interventions
GroupInterventionDescription
Single arm, three cohortsMylotargIdarubicin, cytarabine, Mylotarg.
Primary Outcome Measures
NameTimeMethod
Complete Remission of the Disease28 days after chemotherapy

Rate of patients that have obtained complete remission. Complete remission is defined as, bone marrow normocellular or slightly hypocellular with proportion of blasts \<5%, including the erythroid cell count (and including promonocytes in case of M5), no Auer rods, no extramedullary leukemia, neutrophils and platelets rising. The persistence of minimal residual disease in immunophenotypic study will not invalidate the standard cytogenetic complete remission.

Secondary Outcome Measures
NameTimeMethod
Relapse After 6 Months6 months from complete remission

Rate of patients that have relapse after 6 months of obtained complete remission.

Survival After 6 Months6 months after complete remission

rate of patients alive within 6 months of obtained complete remission

Mortality at InductionWeekly during treatment, at third month and at 6 months after last administration of Mylotarg

all deaths occurring after the first administration of MylotargTM until the time of transplantation with no leukemic relapse has occurred, and all deaths in the 6 first months after administration of the second dose of MylotargTM with no leukemic relapse occurred.

Capacity to Obtain Hematopoietic Progenitor Cells (HPC) for Autotransplantation - DATA NOT COLLECTEDOne month before transplant, expected at 9 months after end of treatment.

Rate of patients with capacity to obtain hematopoietic progenitor cells (HPC) for autotransplantation. This analysis has not been performed, because data were not available in sites.

Secondary Toxicity to Mylotarg(R)Baseline, weekly during treatment and at month 3 and month 6 after first induction.

Hematological toxicity, hepatic and gastrointestinal toxicity, fever and infections, pulmonary complications, duration of hospitalization

Trial Locations

Locations (6)

Hospital de la Santa Creu i Sant Pau

🇪🇸

Barcelona, Spain

Hospital Germans Trias i Pujol

🇪🇸

Badalona, Barcelona, Spain

Hospital Clinico Universitario de Salamanca

🇪🇸

Salamanca, Spain

Hospital Clinic Barcelona

🇪🇸

Barcelona, Spain

Hospital Universitario Virgen del Rocio

🇪🇸

Sevilla, Spain

Hospital Clínico Universitario de Valencia

🇪🇸

Valencia, Spain

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