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Platform Study of Novel Ruxolitinib Combinations in Myelofibrosis Patients

Registration Number
NCT04097821
Lead Sponsor
Novartis Pharmaceuticals
Brief Summary

The purpose of this study is to investigate the safety, pharmacokinetics and preliminary efficacy of combination treatment of ruxolitinib with 5 novel compounds: siremadlin, crizanlizumab, sabatolimab, rineterkib and NIS793 in myelofibrosis (MF) subjects.

Detailed Description

This open-label, multi-center, phase Ib/II platform study design consists of 3 parts. Part 1 is a phase Ib dose escalation and safety run-in for the 5 novel agents in combination with ruxolitinib to assess safety, tolerability and to confirm recommended Phase II dose. Dose escalation cohorts are treated with ruxoltinib + siremadlin or ruxolitinib + rineterkib. Safety run in cohorts are treated with either ruxolitinb + sabatolimab, ruxolitinb + crizanlizumab or ruxolitinib + NIS793 Parts 2 and 3 are phase II selection and expansion respectively, to assess preliminary efficacy of the combination treatments from Part 1 that are evaluated as safe and tolerable. The number of combination treatment arms opening in part 2 will depend on the results of Part 1. In Part 2, an interim analysis was planned to determine if combination treatment (s) can be expanded in Part 3.

In June 2022, Novartis decided to permanently halt the study enrollment in all ongoing parts (part 1 and part 2) and part 3 (expansion) will not be initiated. With protocol amendment 8, an extension treatment phase of 12 cycles is added in Part 1 to allow access to the combination treatment for ongoing subjects deriving clinical benefit. in consideration of the enrollment halt, Parts 2 and 3 objectives will not be pursued and Part 1 objectives are updated accordingly.

Recruitment & Eligibility

Status
COMPLETED
Sex
All
Target Recruitment
45
Inclusion Criteria
  • Subjects have diagnosis of primary myelofibrosis (PMF) according to the 2016 World Health Organization (WHO) criteria, or diagnosis of post-essential thrombocythemia (ET) (PET-MF) or post-polycythemia vera (PV) myelofibrosis (PPV-MF) according to the International Working Group for Myelofibrosis Research and Treatment (IWG-MRT) 2007 criteria
  • Palpable spleen of at least 5 cm from the left costal margin (LCM) to the point of greatest splenic protrusion or enlarged spleen volume of at least 450 cm3 per MRI or CT scan at baseline (a MRI/CT scan up to 8 weeks prior to first dose of study treatment can be accepted).
  • Have been treated with ruxolitinib for at least 12 weeks prior to first dose of study treatment
  • Are stable (no dose adjustments) on the prescribed ruxolitinib dose (between 5 and 25 mg twice a day (BID)) for ≥ 4 weeks prior to first dose of study treatment

Extension treatment phase inclusion criteria:

  • Signed consent for the extension treatment phase
  • ongoing in the core treatment phase
  • demonstrates clinical benefit of treatment in core treatment phase per investigator's assessment.

Core treatment phase

Exclusion Criteria
  • Not able to understand and to comply with study instructions and requirements.
  • Received any investigational agent for the treatment of MF (except ruxolitinib) within 30 days of first dose of study treatment or within 5 half-lives of the study treatment, whichever is greater
  • Peripheral blood blasts count of > 10%.
  • has documented severe hypersensitivity reactions/immunogenicity (IG) to a prior biologic product or Received a monoclonal antibody (Ab) or immunoglobulin-based agent within 1 year of screening in NIS793, crizanlizumab or sabatolimab arms, or in rineterkib or siremadlin arms within <=4 weeks of screening or <=5 half-lives whichever is shorter
  • Splenic irradiation within 6 months prior to the first dose of study drug
  • Received blood platelet transfusion within 28 days prior to first dose of study treatment.

Extension treatment phase Exclusion Criteria:

  • meets any of study treatment discontinuation criteria
  • current evidence of treatment failure per investigator, following treatment in core treatment phase
  • enrolled in another interventional study
  • evidence of non-compliance to study procedures or withdrew consent in core treatment phase
  • currently has unresolved toxicities for which study treatment has been interrupted in the core treatment phase
  • local access to alternative myelofibrosis treatment including those currently under investigation in clinical trials as assessed suitable in the opinion of the investigator.

Other protocol-defined Inclusion/Exclusion criteria may apply.

Study & Design

Study Type
INTERVENTIONAL
Study Design
PARALLEL
Arm && Interventions
GroupInterventionDescription
Part 1 Arm 2: Ruxolitinib + CrizanlizumabCrizanlizumabSafety run-in of crizanlizumab added to existing stable dose of ruxolitinib
Part 1 Arm 3: Ruxolitinib + SabatolimabRuxolitinibSafety run-in of Sabatolimab added to existing stable dose of ruxolitinib
Part 1 Arm 3: Ruxolitinib + SabatolimabSabatolimabSafety run-in of Sabatolimab added to existing stable dose of ruxolitinib
Part 2 Arm 1: Ruxolitinib + SiremadlinRuxolitinibSiremadlin added to existing stable dose of ruxolitinib
Part 2 Arm 1: Ruxolitinib + SiremadlinSiremadlinSiremadlin added to existing stable dose of ruxolitinib
Part 2 Arm 6: Ruxolitinib monotherapyRuxolitinibExisting stable dose of ruxolitinib as control for Part 2
Part 2 Arm 2: Ruxolitinib + CrizanlizumabRuxolitinibCrizanlizumab added to existing stable dose of ruxolitinib
Part 2 Arm 4: Ruxolitinib + RineterkibRuxolitinibRineterkib added to existing stable dose of ruxolitinib
Part 2 Arm 5: Ruxolitinib + NIS793NIS793NIS793 added to existing stable dose of ruxolitinib
Part 1 Arm 5: Ruxolitinib + NIS793RuxolitinibSafety run-in of NIS793 added to existing stable dose of ruxolitinib
Part 1 Arm 5: Ruxolitinib + NIS793NIS793Safety run-in of NIS793 added to existing stable dose of ruxolitinib
Part 1 Arm 1: Ruxolitinib + SiremadlinRuxolitinibDose escalation of siremadlin added to existing stable dose of ruxolitinib
Part 1 Arm 2: Ruxolitinib + CrizanlizumabRuxolitinibSafety run-in of crizanlizumab added to existing stable dose of ruxolitinib
Part 2 Arm 3: Ruxolitinib + SabatolimabSabatolimabSabatolimab added to existing stable dose of ruxolitinib
Part 3 Arm 1: Ruxolitinib + Compound XRuxolitinibCompound from Part 2 (to be confirmed) added to existing stable dose of ruxolitinib
Part 2 Arm 3: Ruxolitinib + SabatolimabRuxolitinibSabatolimab added to existing stable dose of ruxolitinib
Part 3 Arm 2: Ruxolitinib cessationRuxolitinibCompound from Part 2 added to existing stable dose of ruxolitinib for 3 cycles followed by compound monotherapy
Part 3 Arm 3: Ruxolitinib monotherapyRuxolitinibExisting stable dose of ruxolitinib as control for Part 3
Part 1 Arm 4: Ruxolitinib + RineterkibRuxolitinibDose escalation of Rineterkib added to existing stable dose of ruxolitinib
Part 2 Arm 5: Ruxolitinib + NIS793RuxolitinibNIS793 added to existing stable dose of ruxolitinib
Part 1 Arm 1: Ruxolitinib + SiremadlinSiremadlinDose escalation of siremadlin added to existing stable dose of ruxolitinib
Part 2 Arm 2: Ruxolitinib + CrizanlizumabCrizanlizumabCrizanlizumab added to existing stable dose of ruxolitinib
Part 1 Arm 4: Ruxolitinib + RineterkibRineterkibDose escalation of Rineterkib added to existing stable dose of ruxolitinib
Part 2 Arm 4: Ruxolitinib + RineterkibRineterkibRineterkib added to existing stable dose of ruxolitinib
Primary Outcome Measures
NameTimeMethod
Incidence of dose limiting toxicities within the first 2 cyclesBaseline to the end of Cycle 2 (6 or 8 weeks)

Incidence and severity of dose limiting toxicities within the first 2 cycles (6 or 8 weeks) in Part 1 of the study

Response rate at the end of cycle 6 or cycle 8Baseline to the end of Cycle 6 or 8 (24 weeks)

Composite of anemia improvement (hemoglobin level) and no spleen volume progression and no symptom worsening in Part 2 and Part 3 of the study. For a subject to be considered a responder, all three components of the composite have to be fulfilled

Secondary Outcome Measures
NameTimeMethod
Percentage of subjects achieving an improvement in hemoglobin level of ≥ 1.5 g/dL from baselineBaseline to the end of Cycle 6 or 8 (24 weeks), and end of Cycle 12 or 16 (48 weeks)

Percentage of subjects achieving an improvement in hemoglobin level of at least \>= 1.5 g/dL from baseline at each time point in Part 2 and Part 3 of the study.

Percentage of subjects achieving an improvement in hemoglobin level of at least >= 2.0 g/dL from baselineBaseline to the end of Cycle 6 or 8 (24 weeks), and end of Cycle 12 or 16 (48 weeks)

Percentage of subjects achieving an improvement in hemoglobin level of at least \>= 2.0 g/dL from baseline at each time point in Part 2 and Part 3 of the study.

Change in spleen length from baselineBaseline to day 1 and day 15 of Cycle 1, 2 and 3, day 1 of all subsequent cycles, and the end of 12 or 16 cycles (48 weeks)

Change in spleen length measured in centimeters by manual palpation summarized at each time point using descriptive statistics in Part 1 (core and extension), Part 2 and Part 3 of the study

Change in spleen volume from baselineBaseline to the end of Cycle 6 or 8 (24 weeks), the end of Cycle 12 or 16 (48 weeks) and at the end of treatment if not performed in the past 12 weeks (up to 48 weeks)

Change in spleen volume measured by magnetic resonance imaging (MRI) or computed tomography (CT) from baseline summarized at each time point using descriptive statistics, including proportions of subjects who achieved (i) at least 35% spleen volume reduction and (ii) at least 25% spleen volume reduction at the end of Cycle 6 (or 8 for NIS793 arm) (24 weeks) from baseline and, at the end of Cycle 12 (or 16 in NIS793 arm) (48 weeks) from baseline respectively in Part 1 (core and extension) Part 2 and Part 3 of the study

Percentage of subjects achieving an improvement in bone marrow fibrosis of ≥ 1 grade from baselineBaseline to the end of Cycle 6 or 8 (24 weeks), the end of Cycle 12 or 16 (48 weeks) and at the end of treatment if not performed in the past 12 weeks (up to 48 weeks)

Percentage of subjects achieving an improvement in bone marrow fibrosis of \>= 1 grade at each time point will be summarized in Part 2 and Part 3 of the study.

Time to reach maximum (peak) plasma, blood, serum or other body fluid drug concentration after single dose administration (Tmax)Days 1 and 5 of Cycle 1 and 2 for siremadlin and ruxolitinib, and Cycle 1 and Cycle 3 for crizanlizumab, sabatolimab and NIS793, and Days 1 and 15 of Cycle 1 for LTT462

Tmax for each investigational drug in Part 1, Part 2 and Part 3 of the study

Concentration versus time profileDays 1 and 5 of Cycle 1 and 2 for siremadlin and ruxolitinib, and Cycle 1 and Cycle 3 for crizanlizumab, sabatolimab and NIS793, and Days 1 and 15 of Cycle 1 for LTT462

Concentration versus time profile for each investigational drug in Part 1, Part 2 and Part 3 of the study

Change in symptoms of MFSAF v4.0 from baselineBaseline to day 1 of Cycle 1, day 1 of all subsequent cycles of treatment (each cycle is 28 days except for arms containing NIS793, which are 21 days), as well as the end of treatment visit (approximately 52 weeks)

Change in total symptom scores (TSS) assessed by the Myelofibrosis (MF Symptom Assessment Form version 4.0 (MFSAF v4.0) at each time point in Part 2 and Part 3 of the study. The MFSAF v4.0 questionnaire focuses on the 7 core symptoms of MF: fatigue, night sweats, pruritus, abdominal discomfort, pain under the ribs on the left side, early satiety and bone pain. Subjects record symptom severity at it worst for each of the 7 symptoms on an 11-point numeric rating scale, from 0 (absent) to 10 (worst imaginable). The Total Symptom Score (TSS) is the sum of all the scores for all 7 symptoms.

Change in symptoms of EORTC QLQ-C30 from baselineBaseline to day 1 of Cycle 1, day 1 of all subsequent cycles of treatment (each cycle is 28 days except for arms containing NIS793, which are 21 days), as well as the end of treatment visit (approximately 52 weeks)

Change in symptom scores assessed by European Organization for Research and Treatment of Cancer 30-item core quality of life questionnaire (EORTC QLQ C-30) at each time point in Part 2 and Part 3 of the study. The EORTC QLQ-C30 includes 5 functional scales (physical, emotional, social, role, cognitive), eight symptom scales (fatigue, pain, nausea/vomiting, constipation, diarrhea, insomnia, dyspnea, and appetite loss), as well as global health/quality-of-life and financial impact. Subjects respond according to the past week recall period, with the exception of the first 5 questions that represent physical functioning and capture the subject's current status. Raw scores are linearly converted to a 0-100 scale. For functional and global health status/QoL higher scores indicate better QoL and level of functioning; for symptom scales, higher scores indicate greater level of symptoms or difficulties.

Changes in symptoms in MFSAF v4.0 (Part 1) from baselineBaseline to end of Cycle 3 or 4 (week 16), and end of Cycle 6 or 8 (week 24weeks), or end Cycle 12 or 16 (48 weeks)

Change in total symptom scores (TSS) assessed by the Myelofibrosis (MF Symptom Assessment Form version 4.0 (MFSAF v4.0) from baseline including proportion of subjects who achieved at least 50% reduction.

Progression free survival, per progressive splenomegaly, accelerated phase, deteriorating cytopenia, leukemic transformation or death from any causeBaseline to disease progression, which is up to 24 weeks for Part 1 or through study completion, an average of 1 year, for Part 2 and Part 3

Progressive splenomegaly is assessed by increasing spleen volume (by MRI/CT) of ≥ 25% from baseline. Accelerated phase: a circulating peripheral blood blast content of \> 10% but \< 20% confirmed after 2 weeks.

Deteriorating cytopenia (dCP) independent from treatment defined for all patients by platelet count \< 35 x10\^9/L or neutrophil count \< 0.75 x 10\^9/L that lasts for at least 4 weeks.

Leukemic transformation, a peripheral blood blast content of ≥ 20% associated with an absolute blast count of ≥ 1x10\^9/L that lasts for at least 2 weeks or a bone marrow blast count of ≥ 20%.

Area under the Plasma Concentration versus Time Curve (AUC)Days 1 and 5 of Cycle 1 and 2 for siremadlin and ruxolitinib, and Cycle 1 and Cycle 3 for crizanlizumab, sabatolimab and NIS793, and Days 1 and 15 of Cycle 1 for LTT462

AUC for each investigational drug in Part 1, Part 2 and Part 3 of the study

Maximum (peak) observed plasma drug concentration (Cmax)Days 1 and 5 of Cycle 1 and 2 for siremadlin and ruxolitinib, and Cycle 1 and Cycle 3 for crizanlizumab, sabatolimab and NIS793, and Days 1 and 15 of Cycle 1 for LTT462

Cmax for each investigational drug in Part 1, Part 2 and Part 3 of the study

Presence and/or concentration of anti-drug antibodyBaseline to 105 days after last study drug administration for crizanlizumab, to 150 days after last study drug administration for sabatolimab, or to 90 days after last study drug administration for NIS793

The presence and titer of anti-drug antibodies for crizanlizumab, sabatolimab and NIS793 in Part 1, Part 2 and Part 3 of the study

Trial Locations

Locations (1)

Novartis Investigative Site

🇬🇧

London, United Kingdom

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