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Effects of Probiotics and Nutritional Status on Exercise-Induced Symptoms, and Endurance Performance in Ultra-Endurance Athletes

Not Applicable
Completed
Conditions
Ultra-endurance Athletes With Gastrointestinal Symptoms
Interventions
Dietary Supplement: Oral Rehydration Salts only
Dietary Supplement: Oral Rehydration Salts supplemented with L. Rhamnosus GG
Registration Number
NCT04779281
Lead Sponsor
Istanbul Medeniyet University
Brief Summary

Ultra-endurance athletes have been commonly endured extreme conditions during races and training sessions, resulting in exercise-associated clinical symptoms, including gastrointestinal symptoms, dehydration, and elevated oxidative stress. Although these alterations adversely affect sports performance and well-being, there is no certain treatment or consensus on alleviating the exercise-associated clinical symptoms in ultra-endurance athletes. The objective of this study is to determine the effects of oral rehydration salts supplemented with Lactobacillus Rhamnosus GG on exercise-induced gastrointestinal problems, dehydration and oxidative stress in ultra-endurance athletes. Additionally, we aimed to assess the exercise-induced alterations in oxidative stress determined by applying an acute strenuous exercise protocol ((a cycle ergometer (45 min at 65%VO2max) immediately followed by a treadmill test (75% VO2max to exhaustion)) before and after the supplementation period.

Detailed Description

Not available

Recruitment & Eligibility

Status
COMPLETED
Sex
All
Target Recruitment
24
Inclusion Criteria
  • Participants who train at least 15 h per week,
  • Participants who do not have any metabolic disease,
  • Have had at least one gastrointestinal symptom determined by using a Gastrointestinal Symptoms Rating Scale and Bristol Stool Chart Form Scale,
  • Being a non-smoker,
  • Have participated in at least one ultra-endurance race/event (lasts > 4 hr),
  • Taking no vitamins, minerals, dietary supplements, antibiotics, and any medication at least during the three months before the study,
  • (For women) participants with a regular menstrual cycle of physiological length (24-35 days)
Exclusion Criteria
  • Regular tobacco use
  • Inability to adhere to any of the study protocol requirements (i.e. alcohol, caffeine consumption, diet control)
  • Having consumed any supplements and/or medicines for the preceding 3 months period.
  • (For women) participants in menopause or using oral contraceptives

Study & Design

Study Type
INTERVENTIONAL
Study Design
PARALLEL
Arm && Interventions
GroupInterventionDescription
Oral Rehydration Salts supplemented with L. Rhamnosus GGOral Rehydration Salts onlya supplement containing sodium chloride, trisodium citrate, potassium chloride, dextrose, Lactobacillus Rhamnosus GG ATC53103 HN019 strain and fructooligosaccharides
Oral Rehydration Salts onlyOral Rehydration Salts supplemented with L. Rhamnosus GGA supplement containing sodium chloride, trisodium citrate, potassium chloride, dextrose, microcrystalline cellulose
Primary Outcome Measures
NameTimeMethod
Changes of the gut microbiome after a 4-week supplementation periodChange from Baseline Gut Microbiome at 4 weeks

- To assess the profile of gut flora (% abundance of various bacterial phyla, families, genera and species) at baseline and after supplementation in ultra-endurance athletes, using a 16S ribosomal RNA sequencing

Secondary Outcome Measures
NameTimeMethod
Changes in blood markers of exercise-associated oxidative stress (8-isoprostaglandin 2Fa, Total Antioxidant Capacity, and Total Oxidant Capacity in plasma)4 weeks

- Blood samples were collected pre-and post-exercise applied before and after supplementation to evaluate the change in exercise-induced oxidative stress in plasma. Plasma concentrations of 8-isoprostaglandin 2Fa, Total Antioxidant Capacity, and Total Oxidant Capacity were analysed using ELISA kits.

Trial Locations

Locations (1)

Centre of Athlete Training and Health Research

🇹🇷

Ankara, Turkey

Centre of Athlete Training and Health Research
🇹🇷Ankara, Turkey
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