Survival of bacteria on the ocular surface following double application of povidone-iodine before cataract surgery
Not Applicable
Completed
- Conditions
- EndophthalmitisEye - Diseases / disorders of the eye
- Registration Number
- ACTRN12608000187347
- Lead Sponsor
- The Queen Elizabeth Hospital
- Brief Summary
Not available
- Detailed Description
Not available
Recruitment & Eligibility
- Status
- Completed
- Sex
- All
- Target Recruitment
- 72
Inclusion Criteria
Unilateral phacoemulsification surgery for cataract
Exclusion Criteria
Allergy to povidone-iodine, active ocular infection, antibiotic therapy in preceding three weeks
Study & Design
- Study Type
- Observational
- Study Design
- Not specified
- Primary Outcome Measures
Name Time Method Bacterial recontamination of the conjunctiva following the first application of povidone iodine. This will be assessed by comparing cultures of swabs taken after the first application of povidone-iodine, with cultures of swabs taken just prior to the second application of povidone-iodine (about 15 minutes later). Bacterial recontamination could occur during this time. Each swab specimen will be put in a 1mL container of normal saline, from which 300 microlitres (100 microlitres, 3 times separately) is removed by automatic pipette and plated on blood agar (2 plates) as well as chocolate agar (1 plate). Each plate has 100 microlitres of broth applied to it. These plates will be sent for incubation within three hours of specimen collection. Specimens plated on blood agar will be incubated at 35 degrees centigrade a) aerobically, and b) anaerobically. The specimen on chocolate agar will be incubated at 35 degrees centigrade in 5% CO2.[Cultures assessed at three days]
- Secondary Outcome Measures
Name Time Method o bacterial contamination following the first application of povidone-iodine. Swabs are cultured and assessed as in primary outcome.[Cultures assessed at three days]