Impact of Sprint Stair Climbing "Snacks" on Markers of Metabolism and Vascular Function

Not Applicable

Completed

• Conditions: Physical Activity; Diet Modification
• Interventions: Behavioral: Low-carbohydrate Sedentary; Behavioral: High-Carbohydrate Active; Behavioral: High-Carbohydrate Sedentary

• Registration Number: NCT03374436
• Lead Sponsor: University of British Columbia

Brief Summary

Prolonged sitting has been shown to impair metabolism and vascular function. The overall purpose of this study is to determine if breaking up prolonged (9 hours) of sitting with brief (\~20 second) stair climbing exercise "snacks" can improve markers of metabolic control and vascular health in healthy young male participants and in males or females with overweight/obesity who are at risk of insulin resistance. An additional purpose is to determine if saliva insulin can be used as a valid indicator of blood insulin when measured throughout the day in sedentary and active conditions and when diets with different amounts of carbohydrates are consumed.

Detailed Description

Not available

Study Timeline

• Study Start: 2017-10-01
• Study First Submitted: 2017-12-11
• Study First Submitted QC: 2017-12-11
• Study First Posted: 2017-12-15
• Primary Completion: 2019-06-30
• Study Completion: 2019-08-30
• Status Verified: 2019-09
• Last Update Submitted: 2019-09-24
• Last Update Posted: 2019-09-25

Recruitment & Eligibility

• Status: COMPLETED
• Sex: All
• Target Recruitment: 23

Inclusion Criteria

Non-smoking

Lean Group

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Exclusion Criteria

Diagnosed with diabetes or prediabetes (fasting blood sugar more than 6.0 mmol/l) or any other chronic condition that may impact glucose or insulin levels.

BMI is over 25 kg/m2 Take medications which may affect glucose and insulin levels Allergic to eggs. Smoker Competitive or serious endurance athlete.

Overweight/Obese Group Inclusion Criteria:

Elevated waist circumference (>102 cm for male and >88 cm for female)

Overweight/Obese Group Exclusion Criteria:

Diagnosed with diabetes (fasting blood sugar more than 7.0 mmol/l) Take medications which may affect glucose and insulin levels Allergic to eggs. Smoker Competitive or serious endurance athlete.

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Study & Design

• Study Type: INTERVENTIONAL
• Study Design: CROSSOVER

Arm && Interventions

Group	Intervention	Description
Low-Carbohydrate Sedentary	Low-carbohydrate Sedentary	Participants will remain seated for 9 hours (except for using the restroom) while consuming a low-carbohydrate diet (3 meals)
High-Carbohydrate Active	High-Carbohydrate Active	Participants will remain seated for 9 hours (except for using the restroom) while consuming a high-carbohydrate diet (3 meals) but will complete 8 stair climbing sprint "snacks" once per hour involving ascending 3 flights of stairs at a vigorous pace (\~20 seconds each).
High-Carbohydrate Sedentary	High-Carbohydrate Sedentary	Participants will remain seated for 9 hours (except for using the restroom) while consuming a high-carbohydrate diet (3 meals)

Primary Outcome Measures

Name	Time	Method
Plasma insulin area under the curve	Measured across 9 hours of each intervention day	The plasma insulin concentration measured by enzyme-linked immunosorbent assay area under the curve will be measured using the trapezoidal rule.

Secondary Outcome Measures

Name	Time	Method
Cognitive function	Measured in the morning (time 0.5 hours) and afternoon (time 6 hours) each intervention day	Cognitive function measured by Computerized Neurocognitive Assessment Software (CNS) Vital Signs test battery
Saliva insulin area under the curve	Measured across 9 hours of each intervention day	The saliva insulin concentration measured by enzyme-linked immunosorbent assay area under the curve will be measured using the trapezoidal rule.
Plasma glucose area under the curve	Measured across 9 hours of each intervention day	The plasma glucose concentration measured by hexokinase method area under the curve will be measured using the trapezoidal rule.
Plasma triglyceride area under the curve	Measured across 9 hours of each intervention day	The plasma triglyceride concentration measured by biochemical assay area under the curve will be measured using the trapezoidal rule.
Plasma non-esterified fatty acids area under the curve	Measured across 9 hours of each intervention day	The plasma non-esterified fatty acid concentration measured by biochemical assay area under the curve will be measured using the trapezoidal rule.
Femoral artery flow-mediated dilation	Measured in the morning (time 0 hours) and afternoon (time 8.5 hours) each intervention day	Flow-mediated dilation of femoral artery measured by vascular ultrasound
Cerebrovascular function	Measured in the morning (time 0.5 hours) and afternoon (time 6 hours) each intervention day	Cerebrovascular function measured by transcranial doppler ultrasound

Trial Locations

Locations (1)

University of British Columbia Okanagan; 🇨🇦 Kelowna, British Columbia, Canada