Salivary Insulin Profiles Throughout the Day in Healthy Humans
试验速览
- 阶段
- 不适用
- 状态
- 已完成
- 入组人数
- 23
- 试验地点
- 2
- 主要终点
- Plasma insulin area under the curve
研究概览
简要总结
Prolonged sitting has been shown to impair metabolism and vascular function. The overall purpose of this study is to determine if breaking up prolonged (9 hours) of sitting with brief (~20 second) stair climbing exercise "snacks" can improve markers of metabolic control and vascular health in healthy young male participants and in males or females with overweight/obesity who are at risk of insulin resistance. An additional purpose is to determine if saliva insulin can be used as a valid indicator of blood insulin when measured throughout the day in sedentary and active conditions and when diets with different amounts of carbohydrates are consumed.
研究设计
- 研究类型
- Interventional
- 分配方式
- Randomized
- 干预模型
- Crossover
- 主要目的
- Other
- 盲法
- None
盲法说明
It is not possible to mask the exercise or diet interventions.
入排标准
- 年龄范围
- 18 Years 至 65 Years（Adult, Older Adult）
- 性别
- All
- 接受健康志愿者
- 是
入选标准
- •Non-smoking
排除标准
- •Diagnosed with diabetes or prediabetes (fasting blood sugar more than 6.0 mmol/l) or any other chronic condition that may impact glucose or insulin levels.
- •BMI is over 25 kg/m2 Take medications which may affect glucose and insulin levels Allergic to eggs. Smoker Competitive or serious endurance athlete.
- •Overweight/Obese Group Inclusion Criteria:
- •Elevated waist circumference (>102 cm for male and >88 cm for female)
- •Overweight/Obese Group Exclusion Criteria:
- •Diagnosed with diabetes (fasting blood sugar more than 7.0 mmol/l) Take medications which may affect glucose and insulin levels Allergic to eggs. Smoker Competitive or serious endurance athlete.
研究组 & 干预措施
High-Carbohydrate Sedentary
Participants will remain seated for 9 hours (except for using the restroom) while consuming a high-carbohydrate diet (3 meals)
干预措施: High-Carbohydrate Sedentary (Behavioral)
High-Carbohydrate Active
Participants will remain seated for 9 hours (except for using the restroom) while consuming a high-carbohydrate diet (3 meals) but will complete 8 stair climbing sprint "snacks" once per hour involving ascending 3 flights of stairs at a vigorous pace (~20 seconds each).
干预措施: High-Carbohydrate Active (Behavioral)
Low-Carbohydrate Sedentary
Participants will remain seated for 9 hours (except for using the restroom) while consuming a low-carbohydrate diet (3 meals)
干预措施: Low-carbohydrate Sedentary (Behavioral)
结局指标
主要结局
Plasma insulin area under the curve
时间窗: Measured across 9 hours of each intervention day
The plasma insulin concentration measured by enzyme-linked immunosorbent assay area under the curve will be measured using the trapezoidal rule.
次要结局
- Saliva insulin area under the curve(Measured across 9 hours of each intervention day)
- Plasma glucose area under the curve(Measured across 9 hours of each intervention day)
- Plasma triglyceride area under the curve(Measured across 9 hours of each intervention day)
- Plasma non-esterified fatty acids area under the curve(Measured across 9 hours of each intervention day)
- Femoral artery flow-mediated dilation(Measured in the morning (time 0 hours) and afternoon (time 8.5 hours) each intervention day)
- Cerebrovascular function(Measured in the morning (time 0.5 hours) and afternoon (time 6 hours) each intervention day)
- Cognitive function(Measured in the morning (time 0.5 hours) and afternoon (time 6 hours) each intervention day)
研究者
Jonathan Little
Associate Professor
University of British Columbia