SubsTRate OxidatioN in Girls

Not Applicable

Completed

• Conditions: Overweight Adolescents
• Interventions: Other: Acute exercise

• Registration Number: NCT04483921
• Lead Sponsor: Pennington Biomedical Research Center

Brief Summary

This study will examine race differences in total energy expenditure and respiratory quotient (RQ) during and after exercise, compared to a sedentary control condition, in adolescent girls who are classified as overweight. In addition, subjective appetite and objective energy intake will be measured throughout the assessment periods.

Detailed Description

Aim 1: Measure 24-hour energy expenditure and its components (resting, activity, and sleep energy expenditure) and compare differences between African-American and White girls.

Hypothesis: Total, resting, and sleep energy expenditure will be lower in African-American relative to White girls. Activity energy expenditure will be equivalent per the study design.

Aim 2: Measure and compare substrate oxidation and associations with appetite (i.e., hunger, desire to eat) and energy intake between African-American and White girls.

Hypothesis: African-American girls will have a higher RQ at rest, during exercise, and following exercise, and RQ will be positively associated with subjective appetite ratings and energy intake.

Study Timeline

• Study First Submitted: 2020-07-14
• Study Start: 2020-07-15
• Study First Submitted QC: 2020-07-20
• Study First Posted: 2020-07-23
• Primary Completion: 2023-09-02
• Study Completion: 2023-10-20
• Status Verified: 2024-04
• Last Update Submitted: 2024-04-01
• Last Update Posted: 2024-04-02

Recruitment & Eligibility

• Status: COMPLETED
• Sex: Female
• Target Recruitment: 14

Inclusion Criteria

• Female
• Age 13-17 years, inclusive
• Achieved menarche based on parent- and/or self-report
• Overweight based on BMI percentile for age and sex (≥ 85th and < 95th)
• Self-identification as non-Hispanic White or African-American
• Willing to maintain current physical activity, sleep schedule, and dietary habits during the study
• Willing to comply with study procedures

Exclusion Criteria

• Contraindications to exercise testing as defined by the American College of Sports Medicine (i.e., medical history of cardiovascular, pulmonary, or physical conditions that affect the ability to exercise)
• Food allergies
• Dietary restrictions
• Unwilling to consume study foods
• History of polycystic ovary syndrome (PCOS)
• Current amenorrhea or other menstrual cycle irregularities
• Pregnant or currently breastfeeding
• Current anemia
• Use of medications affecting metabolism or sleep
• Inability to properly or safely complete the study procedures per the investigators' discretion

Study & Design

• Study Type: INTERVENTIONAL
• Study Design: CROSSOVER

Arm && Interventions

Group	Intervention	Description
Exercise	Acute exercise	Participants will complete a prescribed exercise bout.

Primary Outcome Measures

Name	Time	Method
Sedentary Day Substrate oxidation	24 hours on Sedentary Day	Non-protein respiratory quotient (i.e., carbohydrate and fat utilization) as measured by the metabolic chamber
Exercise Day Substrate oxidation	24 hours on Exercise Day	Non-protein respiratory quotient (i.e., carbohydrate and fat utilization) as measured by the metabolic chamber
Sedentary Day Energy expenditure	24 hours on Sedentary Day	Total energy expenditure and its components (sleeping, wake \[non-exercise\], activity) as measured by the metabolic chamber
Exercise Day Energy expenditure	24 hours on Exercise Day	Total energy expenditure and its components (sleeping, wake \[non-exercise\], activity) as measured by the metabolic chamber

Secondary Outcome Measures

Name	Time	Method
Sedentary Day Appetite	24 hours on Sedentary Day	Overall subjective appetite and its components (hunger, fullness, desire to eat, prospective food consumption, satisfaction, food wanting) assessed by visual analog scales (0-100 mm) during the metabolic chamber assessments
Exercise Day Appetite	24 hours on Exercise Day	Overall subjective appetite and its components (hunger, fullness, desire to eat, prospective food consumption, satisfaction, food wanting) assessed by visual analog scales (0-100 mm) during the metabolic chamber assessments
Exercise Day Energy Intake	24 hours on Exercise Day	Objectively measured food intake during the metabolic chamber assessments will be converted to energy intake in kilocalories
Sedentary Day Energy Intake	24 hours on Sedentary Day	Objectively measured food intake during the metabolic chamber assessments will be converted to energy intake in kilocalories

Trial Locations

Locations (1)

Pennington Biomedical Research Center; 🇺🇸 Baton Rouge, Louisiana, United States