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Evaluation of SODB® in Metabolic Adaptations in Overweight Women

Not Applicable
Conditions
Overweight
Interventions
Dietary Supplement: Placebo
Dietary Supplement: SODB Dimpless
Other: caloric restriction
Registration Number
NCT02667691
Lead Sponsor
Bionov
Brief Summary

The objective of this study is to evaluate the influence of a 3 months SODB® supplementation in adipose tissue modulations of overweight women, in comparison to a placebo.

Detailed Description

Beneficial experimental results have already been obtained with SODB®. That is why, the investigators expect several effects of SODB® here. Indeed, this clinical study could show that SODB® is able to induce endogenous antioxidant defence, and then reduce oxidative stress generally observed in overweight adipose tissue.

Moreover, this study could evaluate the impact of a decrease in oxidative stress on the others overweight-induced disorders, such as insulin resistance, inflammation, fibrosis, lipolyse alterations.

Recruitment & Eligibility

Status
UNKNOWN
Sex
Female
Target Recruitment
32
Inclusion Criteria
  • overweight women (IMC 25-30 kg/m2)
  • stable weight (variation <5% over the last 3 months)
  • age between 25 and 50 years old
  • cellulite grade ≥ 2
  • women with genital activity
  • regular menstrual cycles (28 ± 2 days over the last 3 months)
  • women with efficient contraception (oral, intra-uterine, ligature trunks or other surgery) - same contraception over the last 3 months
  • women having given her free, informed and express consent.
  • affiliated with a social security insurance or beneficiary of such an insurance system.
  • glycemia < 1,26g/l
  • Arterial blood pression < 140/90 mm Hg or stable for at least 2 months
  • LDL cholesterol <1,90g/l
  • triglycerides <2,50g/l
Exclusion Criteria
  • untreated dyslipidemia
  • unstable hypertension
  • type 1 or 2 diabetes
  • any history of bariatric surgery
  • any history of unstable thyroidian diseases
  • any troubles of hemostasis
  • any allergy to utilized antiseptic, xylocaine, or to one of the compounds of the experimental or placebo product
  • excessive coffee consumption (> 6 cups/day)
  • consumption of drinks containing xanthic bases (> 0.5 l/day)
  • consumption of drinks containing grapefruit juice (> 0.5 l/day)
  • consumption of drinks containing plants (> 2 l/day)
  • medication with draining, lipolytic, anorexigenic activity
  • current or recent (in the previous month) antioxidant supplementation (vitamine A, C, E, beta-carotene, lutein, lycopene, selenium)
  • recent (in the previous year) local anti-cellulite treatment
  • thigh liposuction in the last 2 years
  • under current anticoagulant treatment
  • under current neuroleptic treatment
  • under current corticotherapy (local or systemic) treatment
  • under current diuretic treatment
  • under current antiinflammatory treatment that cannot be interrupted
  • under current treatment which interferes with autonomous nervous system and lipids metabolism
  • triglycérides > 2.50g/l
  • LDL cholesterol >1.90
  • positive serology for hepatitis B, C and HIV
  • consumption of more than 66g/day of alcohol
  • venous fragility not allowing to support catheters during the visits.
  • adult protected by the law
  • any subject who participated to a clinical assay within the 3 months.

Study & Design

Study Type
INTERVENTIONAL
Study Design
PARALLEL
Arm && Interventions
GroupInterventionDescription
SODB Dimpless-caloric restrictioncaloric restrictionThis arm receives daily two capsules of SODB Dimpless 40mg containing 480 UI of superoxide dismutase (SOD), associated with a moderate caloric restriction.
Placebo-caloric restrictionPlaceboThis arm receives daily two capsules Placebo containing excipients only, associated with a moderate caloric restriction.
SODB Dimpless-caloric restrictionSODB DimplessThis arm receives daily two capsules of SODB Dimpless 40mg containing 480 UI of superoxide dismutase (SOD), associated with a moderate caloric restriction.
Placebo-caloric restrictioncaloric restrictionThis arm receives daily two capsules Placebo containing excipients only, associated with a moderate caloric restriction.
Primary Outcome Measures
NameTimeMethod
Change from baseline adipocytes size at 3 monthsEvaluation performed at inclusion day (V1) and after 3 months of treatment at the end of the study (V3).

Evaluation performed by immunohistology in subcutaneous abdominal adipose tissue.

Secondary Outcome Measures
NameTimeMethod
Evaluation of cellulite gradeEvaluation at inclusion day (V1) and after 3 months of treatment at the end of the study (V3).

Evaluation performed by a score.

Evaluation of non esterified fatty acids levelEvaluation at inclusion day (V1) and after 3 months of treatment at the end of the study (V3).

Evaluation performed by non esterified fatty acids measurements.

Evaluation of body weightEvaluation at inclusion day (V1), after 1.5 months of treatment (V2), and after 3 months of treatment at the end of the study (V3).

Evaluation performed by body weight measurements.

Evaluation of cholesterol levelEvaluation at inclusion day (V1) and after 3 months of treatment at the end of the study (V3).

Evaluation performed by cholesterol measurements.

Evaluation of body compositionEvaluation at inclusion day (V1) and after 3 months of treatment at the end of the study (V3).

Evaluation performed by Dual energy X-ray absorptiometry (DEXA).

Evaluation of glycemiaEvaluation at inclusion day (V1) and after 3 months of treatment at the end of the study (V3).

Evaluation performed by glucose measurements.

Evaluation of glycerol levelEvaluation at inclusion day (V1) and after 3 months of treatment at the end of the study (V3).

Evaluation performed by glycerol measurements.

Evaluation of prealbumin levelEvaluation at inclusion day (V1) and after 3 months of treatment at the end of the study (V3).

Evaluation performed by prealbumin measurements.

Evaluation of adipocytes numberEvaluation at inclusion day (V1) and after 3 months of treatment at the end of the study (V3).

Evaluation performed by immunohistology in subcutaneous abdominal and gluteo-femoral adipose tissues.

Adipose tissue secretions of glycerolEvaluation at inclusion day (V1) and after 3 months of treatment at the end of the study (V3).

Evaluation performed by glycerol measurements in subcutaneous abdominal adipose tissue

Adipose tissue secretions of adipokinesEvaluation at inclusion day (V1) and after 3 months of treatment at the end of the study (V3).

Evaluation performed by adipokines measurements in subcutaneous abdominal adipose tissue

Evaluation of insulinemiaEvaluation at inclusion day (V1) and after 3 months of treatment at the end of the study (V3).

Evaluation performed by insulin measurements.

Evaluation of adipocytes sizeEvaluation at inclusion day (V1) and after 3 months of treatment at the end of the study (V3).

Evaluation performed by immunohistology in subcutaneous gluteo-femoral adipose tissue.

Evaluation of triglycerides levelEvaluation at inclusion day (V1) and after 3 months of treatment at the end of the study (V3).

Evaluation performed by triglycerides measurements.

Evaluation of adipokines levelEvaluation at inclusion day (V1) and after 3 months of treatment at the end of the study (V3).

Evaluation performed by adipokines measurements.

Evaluation of C-reactive protein (CRP) levelEvaluation at inclusion day (V1) and after 3 months of treatment at the end of the study (V3).

Evaluation performed by CRP measurements.

Evaluation of albumin levelEvaluation at inclusion day (V1) and after 3 months of treatment at the end of the study (V3).

Evaluation performed by albumin measurements.

Adipose tissue genetic profile modificationsEvaluation at inclusion day (V1) and after 3 months of treatment at the end of the study (V3).

Evaluation of genes (all human known genes tested) differentially expressed in the two arms of subjects performed by DNA microarrays in subcutaneous abdominal adipose tissue.

Evaluation of retinol binding protein (RBP) levelEvaluation at inclusion day (V1) and after 3 months of treatment at the end of the study (V3).

Evaluation performed by RBP measurements.

Evaluation of fibrosisEvaluation at inclusion day (V1) and after 3 months of treatment at the end of the study (V3).

Evaluation performed by immunohIstology (red sirius coloration) in subcutaneous abdominal and gluteo-femoral adipose tissues.

Evaluation of macrophage infiltrationEvaluation at inclusion day (V1) and after 3 months of treatment at the end of the study (V3).

Evaluation performed by immunohistology in subcutaneous abdominal and gluteo-femoral adipose tissues.

Adipose tissue secretions of non esterified fatty acidsEvaluation at inclusion day (V1) and after 3 months of treatment at the end of the study (V3).

Evaluation performed by non esterified fatty acids measurements in subcutaneous abdominal adipose tissue

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